Increased porosity of electrospun hybrid scaffolds improved bladder tissue regeneration

Horst, Maya; Milleret, Vincent; Nötzli, Sarah; Madduri, Srinivas; Sulser, Tullio; Gobet, Rita; Eberli, Daniel

doi:10.1002/jbm.a.34889

Cited by 41 publications

(33 citation statements)

References 32 publications

(107 reference statements)

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“…It is well known that porosity and fiber diameter affect cell migration in electrospun scaffolds. Generally, cell seeded electrospun scaffolds with a percentage of porosity higher than 35% allow adequate migration [37-39]. In the work of Rnjak-Kovacina et al (2011), the authors demonstrated that fibroblasts seeded in electrospun synthetic human elastin was improved when the porosity of the constructs was increased from 14.5 ± 0.8% to 34.4 ± 1.3%.…”

Section: Discussionmentioning

confidence: 99%

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

et al. 2015

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A main goal of tissue engineering is the development of scaffolds that replace, restore and improve injured tissue. These scaffolds have to mimic natural tissue, constituted by an extracellular matrix (ECM) support, cells attached to the ECM, and signaling molecules such as growth factors that regulate cell function. In this study we created electrospun flat sheet scaffolds using different compositions of gelatin and fibrinogen. Smooth muscle cells (SMCs) were seeded on the scaffolds, and proliferation and infiltration were evaluated. Additionally, different concentrations of Transforming Growth Factor-beta2 (TGFβ2) were added to the medium with the aim of elucidating its effect on cell proliferation, migration and collagen production. Our results demostrated that a scafold with a composition of 80% gelatin-20% fibrinogen is suitable for tissue engineering applications since it promotes cell growth and migration. The addition of TGFβ2 at low concentrations (≤1ng/ml) to the culture medium resulted in an increase in SMC proliferation and scaffold infiltration, and in the reduction of collagen production. In contrast, TGFβ2 at concentrations >1ng/ml inhibited cell proliferation and migration while stimulating collagen production. According to our results TGFβ2 concentration has a differential effect on SMC function and thus can be used as a biochemical modulator that can be beneficial for tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

et al. 2015

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“…PLGA has successfully been used as scaffold material to cultivate smooth muscle cells, endothelial cells and fibroblasts [30,34]. Electrospun PLGA scaffolds have good mechanical performance, in vitro degradation ability, and good size stability [35]. However, the hydrophobic nature and lack of binding sites for cell adhesion hinder its wide applications.…”

Section: Introductionmentioning

confidence: 99%

Electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for endothelial cell growth

Zhou

Feng

Yang

et al. 2015

J Mater Sci: Mater Med

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Electrospun scaffolds of silk fibroin (SF) and poly(lactide-co-glycolide) (PLGA) were prepared to mimic the morphology and chemistry of the extracellular matrix. The SF/PLGA scaffolds were treated with ethanol to improve their usability. After ethanol treatment the scaffolds exhibited a smooth surface and uniform fibers. SF transformed from random coil conformation to β-sheet structure after ethanol treatment, so that the SF/PLGA scaffolds showed low hydrophilicity and dissolving rate in water. The mechanical properties and the hydrophilicity of the blended fibrous scaffolds were affected by the weight ratio of SF and PLGA. During degradation of ethanol-treated SF/PLGA scaffolds in vitro, the fibers became thin along with the degradation time. Human umbilical vein endothelial cells (HUVECs) were seeded onto the ethanol-treated nanofibrous scaffolds for cell viability, attachment and morphogenesis studies. These SF/PLGA scaffolds could enhance the viability, spreading and attachment of HUVECs. Based on these results, these ethanol-treated scaffolds are proposed to be a good candidate for endothelial cell growth.

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“…Several natural and synthetic degradable biomaterials have been previously assessed for bladder wall regeneration including bladder acellular matrix, small intestinal submucosa, poly(lactic‐co‐glycolic acid) (PLGA), collagen, and silk fibrinoid . In particular, our laboratory has developed a bilayered hybrid microfibrous scaffold through the direct electrospinning of PLGA on a bladder acellular matrix (BAM) . We have shown that this scaffold is able to promote cell growth and proliferation in vitro and allows for tissue ingrowth and revascularization in a rat cystoplasty model in vivo .…”

Section: Introductionmentioning

confidence: 99%

Polyesterurethane and acellular matrix based hybrid biomaterial for bladder engineering

Horst

Milleret

Noetzli

et al. 2015

J. Biomed. Mater. Res.

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Poly(lactic-co-glycolic acid) (PLGA) based biomaterials for soft tissue engineering have inherent disadvantages, such as a relative rigidity and a limited variability in the mechanical properties and degradation rates. In this study, a novel electrospun biomaterial based on degradable polyesterurethane (PEU) (DegraPol ) was investigated for potential use for bladder engineering in vitro and in vivo. Hybrid microfibrous PEU and PLGA scaffolds were produced by direct electrospinning of the polymer onto a bladder acellular matrix. The scaffold morphology of the scaffold was analyzed, and the biological performance was tested in vitro and in vivo using a rat cystoplasty model. Anatomical and functional outcomes after implantation were analyzed macroscopically, histologically and by cystometry, respectively. Scanning electron microscopy analysis showed that PEU samples had a lower porosity (p < 0.001) and were slightly thinner (p = 0.009) than the PGLA samples. Proliferation and survival of the seeded smooth muscle cells in vitro were comparable on PEU and PLGA scaffolds. After 8 weeks in vivo, the PEU scaffolds exhibited no shrinkage. However, cystometry of the reconstructed bladders exhibited a slightly greater functional bladder capacity in the PLGA group. Morphometric analyses revealed significantly better tissue healing (p < 0.05) and, in particular, better smooth muscle regeneration, as well as a lower rate of inflammatory responses at 8 weeks in the PEU group. Collectively, the results indicated that PEU-hybrid scaffolds promote bladder tissue formation with excellent tissue integration and a low inflammatory reaction in vivo. PEU is a promising biomaterial, particularly with regard to functional tissue engineering of the bladder and other hollow organs. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 658-667, 2017.

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Increased porosity of electrospun hybrid scaffolds improved bladder tissue regeneration

Cited by 41 publications

References 32 publications

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

TGFβ2 differentially modulates smooth muscle cell proliferation and migration in electrospun gelatin-fibrinogen constructs

Electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for endothelial cell growth

Polyesterurethane and acellular matrix based hybrid biomaterial for bladder engineering

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