Increased Plasma 8-Isoprostane Levels in Hypertensive Subjects: the Tsurugaya Project

Hozawa, Atsushi; Ebihara, Satoru; Ohmori, Kenji; Kuriyama, Shinichi; Ugajin, Tomohisa; Koizumi, Yayoi; Suzuki, Yoshinori; Matsui, Toshifumi; Arai, Hiroyuki; Tsubono, Yoshitaka; Sasaki, Hidetada; Tsuji, Ichiro

doi:10.1291/hypres.27.557

Cited by 58 publications

(34 citation statements)

References 18 publications

(13 reference statements)

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“…For instance, F 2 -isoprostanes, a group of LPO products widely used as biomarkers of oxidative stress, are usually found in human plasma at picomolar concentrations (33,34). It is possible that the micromolar concentrations of ascorbylated HNE in human plasma reflect the formation of these vitamin C conjugates in tissues, where intracellular concentrations of ascorbic acid range from 1 to 5 mM, and where HNE can accumulate in membranes at concentrations of 10 M to as high as 5 mM in response to oxidative stress (35).…”

Section: Interactions Of Hpode With Ascorbic Acid and Glutathione (Gsh)mentioning

confidence: 99%

RETRACTED: Vitamin C conjugates of genotoxic lipid peroxidation products: Structural characterization and detection in human plasma

Sowell

Frei

Stevens

2004

Proc. Natl. Acad. Sci. U.S.A.

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α,β-Unsaturated aldehydes such as 4-hydroxy-2-nonenal (HNE) and other electrophilic lipid peroxidation (LPO) products may contribute to the pathogenesis of cancer, cardiovascular diseases, and other age-related diseases by cytotoxic, genotoxic, and proinflammatory mechanisms. The notion that vitamin C (ascorbic acid) acts as a biological antioxidant has been challenged recently by an in vitro study showing that ascorbic acid promotes, rather than inhibits, the formation of genotoxic LPO products from the lipid hydroperoxide, hydroperoxy octadecadienoic acid [Lee, S. H., Oe, T. & Blair, I. A. (2001) Science 292, 2083–2086]. Here, we demonstrate that ascorbic acid acts as a nucleophile and forms Michael-type conjugates with electrophilic LPO products. Several ascorbyl-LPO product conjugates, resulting from the interaction of ascorbic acid with hydroperoxy octadecadienoic acid in vitro, were identified by tandem MS, including ascorbyl conjugates of HNE, 4-oxo-2-nonenal, and presumably, 12-oxo-9-hydroxy-10-dodecenoic acid. The same ascorbyl-LPO product conjugates were detected in human plasma. The concentration of the ascorbyl-HNE conjugate in plasma from 11 healthy subjects was found to be 1.30 ± 0.74 μM (mean ± SD). Our data identify ascorbylation (vitamin C conjugation) as a previously unrecognized, biologically relevant pathway for the elimination of electrophilic LPO products, and have implications for the prevention and treatment of chronic inflammatory diseases, as well as the development of novel biomarkers of oxidative stress

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Section: Interactions Of Hpode With Ascorbic Acid and Glutathione (Gsh)mentioning

confidence: 99%

RETRACTED: Vitamin C conjugates of genotoxic lipid peroxidation products: Structural characterization and detection in human plasma

Sowell

Frei

Stevens

2004

Proc. Natl. Acad. Sci. U.S.A.

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“…Levels of oxidative stress markers, such as 8-isoprostane, increase with an elevation in blood pressure. 22,23 Reducing ROS might be involved in the antihypertensive action mechanism of the active beverage.…”

Section: Discussionmentioning

confidence: 99%

Effects of hydroxyhydroquinone-reduced coffee on vasoreactivity and blood pressure

et al. 2009

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Recent studies suggest that chlorogenic acids, which are the main components of the polyphenol class in coffee, decrease blood pressure, and that hydroxyhydroquinone (HHQ), which is generated by roasting coffee beans, inhibits the antihypertensive effect of chlorogenic acids in brewed coffee. Here, we examined the vasoreactivity and antihypertensive effects of HHQ-reduced coffee in mild hypertension. The study design was a double blind, randomized, placebo-controlled intervention study, with a 4-week run-in period, followed by an 8-week test beverage ingestion period. The subjects were Japanese men and women with mild hypertension and vascular failure, who were not taking any antihypertensive drugs. During the test beverage ingestion period, the subjects ingested either active or placebo HHQ-reduced coffee (chlorogenic acids per 184 ml of coffee: active, 300 mg; and placebo, 0 mg) daily. Subjects were randomly divided into two groups: active group (n¼9) and placebo group (n¼12). In the active beverage group, endothelium-dependent, flow-mediated vasodilation impairment was significantly ameliorated and systolic blood pressure was significantly decreased from the baseline, but not in the placebo group. There were no test beverage consumption-related changes in other parameters that may influence blood pressure, such as pulse, cardiac output, body weight or 24-h urine volume. Ingestion of the active beverage significantly decreased urinary isoprostane levels, suggesting a reduced oxidative stress. These findings indicate that HHQ-reduced coffee decreased blood pressure in subjects with mild hypertension. The decreased blood pressure was associated with improved vascular endothelial function.

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“…The study was performed as a component of the Tsurugaya project (Hozawa et al 2004), a community-based comprehensive geriatric assessment (CGA) conducted in 2002 and 2003 among elderly Japanese subjects living in Tsurugaya district, a suburban area of Sendai City in northern Japan. CGA is a structured approach to measuring physical, mental, and social functioning of elderly people to assess early deterioration to long-term care conditions and to promote healthy and active aging.…”

Section: Study Populationmentioning

confidence: 99%

Determinants of the Utilization of Dental Services in a Community-Dwelling Elderly Japanese Population

Ohi

Sai

Kikuchi

et al. 2009

Tohoku J. Exp. Med.

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Increased Plasma 8-Isoprostane Levels in Hypertensive Subjects: the Tsurugaya Project

Cited by 58 publications

References 18 publications

RETRACTED: Vitamin C conjugates of genotoxic lipid peroxidation products: Structural characterization and detection in human plasma

RETRACTED: Vitamin C conjugates of genotoxic lipid peroxidation products: Structural characterization and detection in human plasma

Effects of hydroxyhydroquinone-reduced coffee on vasoreactivity and blood pressure

Determinants of the Utilization of Dental Services in a Community-Dwelling Elderly Japanese Population

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