Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid

Li, Jie; Li, Bo; Luo, Lilin; Cao, Fulin; Yang, Bowen; Gao, Jing; Yan, Yi‐Lin; Zhang, Gang; Peng, Liang; Hu, Biao

doi:10.1007/s11033-020-05899-1

Cited by 26 publications

(26 citation statements)

References 47 publications

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“…This value was also much higher than in the roots of the mother plants of most other species of sage (0.5–26 mg/g DW), a common source of this metabolite [ 11 , 13 ]. Similar to our results, in transformed roots of S. przewalskii MJA treatment doubled the RA content to 67 mg/g DW relative to the control after 3 days [ 25 ]. Three-day MJA exposure also stimulated RA accumulation in the regenerated shoot cultures of S. virgata by 70% to 103% depending on elicitor concentration [ 23 ].…”

Section: Resultssupporting

confidence: 90%

“…MJA is believed to activate the transcripts of PAL and C4H enzymes from the phenylpropanoid pathway, as well as TAT and HPPR from the tyrosine-derived pathway ( Figure 3 ), thus increasing the rate of biosynthesis of RA in S. miltiorrhiza hairy root culture from 3.25 to 6.02% [ 40 ]. Moreover, in hairy roots of S. przewalskii , after treatment with MJA, various genes coding enzymes of the phenolic acid biosynthesis pathway, such as PAL, 4CL, RAS, HPPR, TAT and CYP98A14, demonstrated a significant increase in expression compared to the controls [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…Both biotic (yeast extract, YE) and abiotic (MJA, metal salt) elicitors stimulated total phenol content in the shoot cultures of S. virgata , wherein MJA was the most effective for SAA accumulation and Ag ions for caffeic acid [ 23 ]. Moreover, MJA doubled the level of RA in hairy roots of S. przewalskii [ 25 ] and S. miltiorrhiza [ 18 ] and increased RA biosynthesis in S. officinalis shoots by 75% [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots of Salvia bulleyana

et al. 2022

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Hairy root cultures are valuable sources of a range of phytochemicals. Among them, Salvia bulleyana root culture is a promising source of polyphenols, especially rosmarinic acid (RA), a phenolic acid depside with pleiotropic activity and a wide application in medicine and cosmetology. The aim of the study was to enhance the culture productivity by finding suitable elicitation protocol and to determine its biological potential in terms of antioxidant, anticancer and antimicrobial properties. The total content of phenols and the levels of particular constituents in root extracts were analyzed using HPLC-PDA. Among four elicitors tested (yeast extract; methyl jasmonate, MJA; trans-anethol; and cadmium chloride), MJA was found to be the most effective. The greatest boost in phenolic production (up to 124.4 mg/g dry weight) was observed after three-day treatment with MJA at 100 µM, with an almost 100% improvement compared to the controls (non-treated root culture). The hydromethanolic extract from the elicited culture exhibited strong antioxidant activity with IC50 values of 11.1 µg/mL, 6.5 µg/mL and 69.5 µg/mL for DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid)) and superoxide anion radical, respectively. Moreover, in concentrations of 0.5–5 mg/mL the extract inhibited the growth of LoVo, AGS and HeLa cell lines, but was safe for the L929 cells up to the concentration of 5 mg/mL. The extract also exhibited moderate antimicrobial activity. Thus, the results confirmed that elicitation can be a beneficial strategy for increase the phenolic acid biosynthesis in hairy roots of S. bulleyana, and that such a highly productive culture can show significant biological potential.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots of Salvia bulleyana

et al. 2022

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“…The secondary metabolites from S. przewalskii therefore attracted great interest towards their phytochemical investigation in recent decades. Intriguingly, abietane-type diterpenoids and phenolic acid derivatives are two major chemical constituents of the roots of S. przewalskii, which also appears in the phytological related S. miltiorrhiza [5][6][7][8][9][10]. Pharmacological studies revealed the beneficial effects of both the crude extracts and chemical constituents of S. przewalskii, including heart-protective, antioxidative, anti-inflammatory, antitussive, and antibacterial properties [4,[11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Regioisomers Salviprolin A and B, Unprecedented Rosmarinic Acid Conjugated Dinorditerpenoids from Salvia przewalskii Maxim

Su¹,

Wu²,

Wu³

et al. 2021

Molecules

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Salvia przewalskii Maxim is a perennial plant from the genus Salvia (family Lamiaceae). The roots of S. przewalskii were long used as a traditional herb to treat blood circulation related illnesses in China. As part of our continuing interest in polycyclic natural products from medicinal plants, two unprecedented adducts comprised of a dinor-diterpenoid and a 9′-nor-rosmarinic acid derivative, linked by a 1,4-benzodioxane motif (1 and 2), were isolated from the roots of S. przewalskii. Their structures were established by extensive spectroscopic approaches including 1D, 2D NMR, and HRFABMS. Their cytotoxic activities against five human tumor cell lines were evaluated.

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“…The well known elicitor methyl jasmonate (MeJA) is a phytohormone that contributes to the hyperproduction of secondary plant metabolites, as it plays an essential role in the regulation of specific genes, involved in plant development and defense mechanisms against different biotic and abiotic stresses ( Ji et al, 2019 ; Baek et al, 2020 ). Although previous studies have examined the effects of MeJA on the production of UA, OA and RA in Lamiaceae family ( Ruffoni et al., 2016 ; Li et al, 2020 ; Yousefian et al, 2020 ), few studies, with the exception of Ocimum basilicum suspension cultures ( Pandey, Singh & Banerjee, 2019 ), have been designed to explore the feasibility of up-scaling the production of these three bioactive metabolites, using an in vitro culture system. Here, we describe the first protocol for the establishment of hairy root culture of L. caulescens and present a comparative analysis of the effects of different concentrations and times of exposure to MeJA on the production of UA, OA, and RA.…”

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

Vergara-Martínez

Estrada‐Soto

Valencia-Díaz

et al. 2021

PeerJ

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Background Ursolic (UA), oleanolic (OA) and rosmarinic (RA) acids are bioactive metabolites found in Lepechinia caulescens that have generated interest for their health benefits, which include antimicrobial, antioxidant, antimutagenic, gastroprotective, antidiabetic, antihypertensive and anti-inflammatory properties, among others. To date, very few attempts have been made to evaluate the potential for simultaneous production of these bioactive compounds, using a biotechnological approach. Hairy root cultures offer a biotechnology approach that can be used to study the factors affecting the biosynthesis and the production of UA, OA and RA. In the current study, we established hairy root cultures of L. caulescens and evaluated the effect of sucrose on biomass accumulation, and the effect of different concentrations and times of exposure of methyl jasmonate (MeJA), on the accumulation of UA, OA and RA. Methods Leaves from plants of L. caulescens were inoculated with Agrobacterium rhizogenes strain ATCC 15834. PCR of rolB gene confirmed the transgenic nature of hairy roots. Hairy roots were subcultured in semisolid MSB5 medium, supplemented with 15, 30, 45 or 60 g/L sucrose and after 4 weeks, dry weight was determined. The accumulation of UA, OA and RA of wild plants and hairy roots were determined by HPLC. Finally, the hairy roots were treated with 0, 100, 200 and 300 µM of MeJA and the content of bioactive compounds was analyzed, after 24, 48 and 72 h. Results High frequency transformation (75%) was achieved, using leaf explants from axenic seedlings, infected with A. rhizogenes. The hairy roots showed an enhanced linear biomass accumulation, in response to the increase in sucrose concentration. The hairy root cultures in MSB5 medium, supplemented with 45 g/L sucrose, were capable to synthesizing UA (0.29 ± 0.00 mg/g DW), OA (0.57 ± 0.00 mg/g DW) and RA (41.66 ± 0.31 mg/g DW), about two, seven and three times more, respectively, than in roots from wild plants. Elicitation time and concentration of MeJA resulted in significant enhancement in the production of UA, OA and RA, with treatments elicited for 24 h, with a concentration of 300 µM of MeJA, exhibiting greatest accumulation. Conclusion This is the first report on development of hairy root cultures of L. caulescens. Future studies should aim towards further improving triterpenes and polyphenolic compound production in hairy roots of L. caulescens, for use in the pharmaceutical and biotechnological industry.

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Increased phenolic acid and tanshinone production and transcriptional responses of biosynthetic genes in hairy root cultures of Salvia przewalskii Maxim. treated with methyl jasmonate and salicylic acid

Cited by 26 publications

References 47 publications

The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots of Salvia bulleyana

The Antioxidant, Cytotoxic and Antimicrobial Potential of Phenolic Acids-Enriched Extract of Elicited Hairy Roots of Salvia bulleyana

Regioisomers Salviprolin A and B, Unprecedented Rosmarinic Acid Conjugated Dinorditerpenoids from Salvia przewalskii Maxim

Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens

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