Increased permeability of the epithelium of middle ear cholesteatoma

Koizumi, Hiroki; Suzuki, Hideaki; Ohbuchi, Toyoaki; Kitamura, Takuro; Hashida, Koichi; Nakamura, Motonobu

doi:10.1111/coa.12332

Cited by 17 publications

(18 citation statements)

References 59 publications

(112 reference statements)

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“…One study revealed increased epithelial permeability of middle ear cholesteatoma compared with the post-auricular skin and external auditory canal skin using MA measurement with catheterization and concluded that the difference was at least partially dependent on the difference of tight junction protein expression. 25 Another study revealed that the electrical impedance of skin, which was calculated from the values measured by this method, was higher than those of the nasal turbinate and nasal polyps and that claudin-1 mRNA levels paralleled electrical impedance values. 26 Although we did not evaluate the expression of tight junction proteins in this study, increased MA in patients with FD was likely caused by decreased expression of tight junction proteins, based on a previous study by Vanheel et al…”

Section: Discussionmentioning

confidence: 96%

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

Ishigami¹,

Matsumura²,

Kasamatsu³

et al. 2017

Gastroenterology

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Section: Discussionmentioning

confidence: 96%

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

Ishigami¹,

Matsumura²,

Kasamatsu³

et al. 2017

Gastroenterology

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“…On the other hand, at the corner where three epithelial cells join, the tight junction is made up of tricellulin. It has been documented that claudin-1 and tricellulin are expressed in the epithelium of the nasal mucosa [9,10,11] as well as in the epidermis [8,12]. The epidermis contains another important barrier protein, filaggrin.…”

Section: Discussionmentioning

confidence: 99%

“…Broadly speaking, the ‘resistor' and ‘capacitor' of the biological tissue correspond to barrier integrity and water content, respectively [25,26]. In this model, the absolute value of electrical impedance (|Z|) is theoretically calculated by the following equation [8]:…”

Section: Discussionmentioning

confidence: 99%

“…The electrical impedance of the turbinate mucosa, nasal polyp, and postauricular skin was measured in vivo, using a tissue conductance meter (AS-TC100; Asahi Biomed Co., Ltd., Yokohama, Japan), as described previously [8]. Surgery was conducted under general anesthesia in all patients, and the measurement was performed before local injection/incision.…”

Section: Methodsmentioning

confidence: 99%

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Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp

Suzuki

Koizumi

Ikezaki

et al. 2015

ORL

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Purpose: We investigated the electrical impedance and expression of tight junction components of the turbinate mucosa, nasal polyp, and normal skin. Procedures: The inferior turbinate and nasal polyp of patients with chronic rhinosinusitis and the postauricular skin of patients with otitis media were examined. Electrical impedance was measured in vivo using a tissue conductance meter. Expressions of claudin-1 and tricellulin were examined by fluorescence immunohistochemistry and quantitative RT-PCR. Results: Electrical impedance was higher in the skin than in the turbinate and polyp, but did not differ between the turbinate and polyp. Immunoreactivities for claudin-1 and tricellulin were seen in the epithelial/epidermal layer. Expression of claudin-1 was higher in the skin than in the turbinate and polyp. The polyp tended to show higher expression of claudin-1 but showed lower expression of tricellulin than the turbinate. The ratio of claudin-1 to tricellulin was highest in the skin and lowest in the turbinate. The correlation between expressions of the two tight junction components was strongly positive in the skin (r = 0.964) and negative (r = -0.527) in the turbinate and polyp. Conclusions: These results suggest that the roles of claudin-1 and tricellulin in barrier function may be complementary, and may thereby maintain a constant level of permeability of the mucosal tissues.

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“…A more recent work showed that a probe inserted through endoscopy could measure mucosal resistance in a short time and that research groups are trying to establish an easy method for measuring mucosal barrier function [38]. A device for measuring mucosal conductance using a probe inserted through endoscopy is also being developed in Japan, a [39]. Such easy procedures for measuring mucosal barrier function are expected to provide a good tool for the diagnosis of NERD and the effect of GERD treatment.…”

Section: Involvement Of Tj Proteins In Diseases Gastroesophageal Reflmentioning

confidence: 99%

Gastrointestinal mucosal barrier function and diseases

2016

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The gastrointestinal mucosal barrier plays an essential role in the separation of the inside of the body from the outside environment. Tight junctions (TJs) are the most important component for construction of a constitutive barrier of epithelial cells, and they regulate the permeability of the barrier by tightly sealing the cell-cell junctions. TJ proteins are represented by claudins, occludin, junctional adhesion molecules, and scaffold protein zonula occludens. Among these TJ proteins, claudins are the major components of TJs and are responsible for the barrier and the polarity of the epithelial cells. Gastrointestinal diseases including reflux esophagitis, inflammatory bowel disease, functional gastrointestinal disorders, and cancers may be regulated by these molecules, and disruption of their functions leads to chronic inflammatory conditions and chronic or progressive disease. Therefore, regulation of the barrier function of epithelial cells by regulating the expression and localization of TJ proteins is a potential new target for the treatment of these diseases. Treatment strategies for these diseases might thus be largely altered if symptom generation and/or immune dysfunction could be regulated through improvement of mucosal barrier function. Since TJ proteins may also modify tumor infiltration and metastasis, other important goals include finding a good TJ biomarker of cancer progression and patient prognosis, and developing TJ protein-targeted therapies that can modify patient prognosis. This review summarizes current understanding of gastrointestinal barrier function, TJ protein expression, and the mechanisms underlying epithelial barrier dysregulation in gastrointestinal diseases.

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Increased permeability of the epithelium of middle ear cholesteatoma

Cited by 17 publications

References 59 publications

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp

Gastrointestinal mucosal barrier function and diseases

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