Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp

Abstract

Purpose: We investigated the electrical impedance and expression of tight junction components of the turbinate mucosa, nasal polyp, and normal skin. Procedures: The inferior turbinate and nasal polyp of patients with chronic rhinosinusitis and the postauricular skin of patients with otitis media were examined. Electrical impedance was measured in vivo using a tissue conductance meter. Expressions of claudin-1 and tricellulin were examined by fluorescence immunohistochemistry and quantitative RT-PCR. Results: Electrical impedance was higher in the skin than in the turbinate and polyp, but did not differ between the turbinate and polyp. Immunoreactivities for claudin-1 and tricellulin were seen in the epithelial/epidermal layer. Expression of claudin-1 was higher in the skin than in the turbinate and polyp. The polyp tended to show higher expression of claudin-1 but showed lower expression of tricellulin than the turbinate. The ratio of claudin-1 to tricellulin was highest in the skin and lowest in the turbinate. The correlation between expressions of the two tight junction components was strongly positive in the skin (r = 0.964) and negative (r = -0.527) in the turbinate and polyp. Conclusions: These results suggest that the roles of claudin-1 and tricellulin in barrier function may be complementary, and may thereby maintain a constant level of permeability of the mucosal tissues.

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

“…25 Another study revealed that the electrical impedance of skin, which was calculated from the values measured by this method, was higher than those of the nasal turbinate and nasal polyps and that claudin-1 mRNA levels paralleled electrical impedance values. 26 Although we did not evaluate the expression of tight junction proteins in this study, increased MA in patients with FD was likely caused by decreased expression of tight junction proteins, based on a previous study by Vanheel et al…”

Endoscopy-Guided Evaluation of Duodenal Mucosal Permeability in Functional Dyspepsia

“…25 Another study revealed that the electrical impedance of skin, which was calculated from the values measured by this method, was higher than those of the nasal turbinate and nasal polyps and that claudin-1 mRNA levels paralleled electrical impedance values. 26 Although we did not evaluate the expression of tight junction proteins in this study, increased MA in patients with FD was likely caused by decreased expression of tight junction proteins, based on a previous study by Vanheel et al 17 …”

Gene Expression Profiles of Circular RNAs and MicroRNAs in Chronic Rhinosinusitis With Nasal Polyps

“…TNF, a complex and important inflammatory factor, induces local production of IgA and stimulates eosinophils, and it plays an important role in the pathogenesis of CRSwNP (Kato et al, 2008;Cho et al, 2015;Shimizu et al, 2016). The integrity of the airway epithelium is a prerequisite for its good barrier function, which depends on the intercellular junctions, including tight junctions and adhesion junctions (Suzuki et al, 2016;Jiao et al, 2018). Studies have shown that the breakdown of tight junctions and adhesion junctions of CRS and the decrease of protein component expression are major factors leading to the occurrence of CRS (Kim et al, 2018;Tian et al, 2018).…”