Increased levels of soluble Fcγ receptor III in gingival fluid from periodontal lesions

Abstract

Enzyme-linked immunosorbent assay was used for determination of the concentration of soluble Fc gamma receptor III (Fc gamma RIII) in 40 samples of gingival fluid obtained from periodontal pockets in 30 patients with periodontitis. The assay was based on a monoclonal immobilized antibody binding Fc gamma RIII and a polyclonal Fc gamma RIII rabbit antibody for its quantification. The results indicate a substantially increased concentration of soluble Fc gamma RIII in gingival fluid as compared to the serum level. This increased concentration of soluble Fc gamma RIII may interfere with phagocytosis and immune homeostasis in the periodontal lesions.

Fcα receptor I (CD89) on neutrophils in periodontal lesions

“…This suggests that neutrophils lose surface FcgRs after the cells move into the periodontal pocket. FcgRIII is likely to be cleaved off by microbial phospholipase C (Bul-kacz & Erbland 1997, Siboo et al 1989), a contention that is supported by the observation of high levels of soluble FcgRIII in gingival fluid (Yuan et al 1999b). This might seriously impair phagocytosis of bacteria by neutrophils in the periodontal pocket.…”

Fcγ receptor polymorphisms and their association with periodontal disease: a meta‐analysis

“…Kobayashi et al (2000b) This study examines the relevance of IgG receptor IIIb (CD16) polymorphism to handling of Porphyromonas gingivalis 13. Yuan et al (1999b) Determination of the levels of soluble Fcg receptor III in gingival fluid from periodontal lesions 14. Fredriksson et al (1999) This study does not examine the distribution of genotypes 15.…”

Humoral immune responses in gingival crevice fluid: local and systemic implications

“…Soluble Fcγ‐binding components, e.g. FcRγIII, have been detected in GCF from periodontal lesions (242). The results showed elevated GCF levels of this molecule when compared to matched sera, suggesting a potential contribution to disrupting local homeostatsis.…”