Increased Levels of Soluble CD14 in Sera of Periodontitis Patients

Hayashi, Joichiro; Masaka, Tamami; Ishikawa, Isao

doi:10.1128/iai.67.1.417-420.1999

Cited by 63 publications

(46 citation statements)

References 35 publications

(37 reference statements)

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“…Increased levels of soluble CD14 were found in the serum of patients with periodontitis compared with individuals without periodontitis. 26 Thus, in the setting of periodontitis, CD14 in the systemic circulation as well as upregulated expression of Cd14 by b cells may both be evident.…”

Section: Discussionmentioning

confidence: 99%

Porphyromonas gingivalis Lipopolysaccharide Upregulates Insulin Secretion From Pancreatic β Cell Line MIN6

Bhat

Ilievski

Unterman

et al. 2014

Journal of Periodontology

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Background A close association between periodontitis and diabetes has been demonstrated in human cross-sectional studies, but an exact relationship between periodontitis and prediabetes has not been established. Previous studies using animal model systems consistently have shown that hyperinsulinemia occurs in animals with periodontitis compared to animals with healthy periodontium (while maintaining normoglycemia). Because bacterial lipopolysaccharide (LPS) plays an important role in the pathogenesis of periodontitis, we hypothesized that LPS may stimulate insulin secretion through a direct effect on β cell function. To test this hypothesis, pancreatic β cell line MIN6 cells were used to determine the effect of Porphyromonas gingivalis (Pg) LPS on insulin secretion. Furthermore, expression of genes altered by Pg LPS in innate immunity and insulin-signaling pathways was determined. Methods MIN6 cells were grown in medium with glucose concentration of normoglycemia (5.5 mM). Pg LPS was added to each well at final concentrations of 50, 200, and 500 ng/mL. Insulin secretion was measured using enzyme-linked immunosorbent assay. Gene expression levels altered by Pg LPS were determined by polymerase chain reaction (PCR) array for mouse innate and adaptive immunity response and mouse insulin-signaling pathways, and results were confirmed for specific genes of interest by quantitative PCR. Results Pg LPS stimulated insulin secretion in the normoglycemic condition by ≈1.5- to 3.0-fold depending on the concentration of LPS. Pg LPS treatment altered the expression of several genes involved in innate and adaptive immune response and insulin-signaling pathway. Pg LPS upregulated the expression of the immune response–related genes cluster of differentiation 8a (Cd8a), Cd14, and intercellular adhesion molecule-1 (Icam1) by about two-fold. LPS also increased the expression of two insulin signaling–related genes, glucose-6-phosphatase catalytic subunit (G6pc) and insulin-like 3 (Insl3), by three- to four-fold. Conclusions We have demonstrated for the first time that Pg LPS stimulates insulin secretion by pancreatic β cell line MIN cells. Pg LPS may have significant implications on the development of β cell compensation and insulin resistance in prediabetes in individuals with periodontitis.

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Section: Discussionmentioning

confidence: 99%

Porphyromonas gingivalis Lipopolysaccharide Upregulates Insulin Secretion From Pancreatic β Cell Line MIN6

Bhat

Ilievski

Unterman

et al. 2014

Journal of Periodontology

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“…In general, increased levels of sCD14 have been found in non-infectious and infectious diseases such as systemic lupus erythematosus (Nockher et al, 1994), rheumatoid arthritis (Horneff et al, 1993), polytraumatised and severely burned patients (Kruger et al, 1991), septic shock (Landmann et al, 1995), malaria (Wenisch et al, 1996), brucellosis (Ayaslioglu et al, 2005), HIV-infection (Lien et al, 1998), and tuberculosis with or without HIV infection (Lawn et al, 2000). Hayashi et al (1999) investigated the concentrations of sCD14 in the sera of patients with adult periodontitis, early-onset periodontitis and compared with periodontally healthy donors. They found that sCD14 concentration in serum was significantly higher in patients with periodontitis than in healthy controls, but did not find significant difference between both clinical forms of periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Estimation of sCD14 levels in saliva obtained from patients with various periodontal conditions

Aristizábal-Cardona

et al. 2008

Oral Diseases

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“…The CD14 −260 SNP has previously been associated of increased risk with myocardial infarction (Hubacek et al 1999) and Crohn's disease (Klein et al 2002). Furthermore, increased serum levels of sCD14 have been associated with periodontitis (Hayashi et al 1999).…”

Section: Modifying Disease Genes In Relation To Periodontitismentioning

confidence: 99%

Identification of genetic risk factors for periodontitis and possible mechanisms of action

Loos¹,

John²,

Laine

2005

J Clinic Periodontology

256

221

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Mutations in the cathepsin C gene (CTSC) have been identified as causal for the Papillon-Lefèvre syndrome (PLS), which includes prepubertal periodontitis (PP). Some CTSC mutations are causal for PP without PLS. No relationship has been demonstrated between CTSC mutations and other forms of periodontitis. Genetic polymorphisms in a candidate gene approach have been explored as risk factors for periodontitis. There is limited evidence that some polymorphisms in the genes encoding interleukins (IL)-1, Fc gamma receptors (Fc gammaR), IL-10 and the vitamin D receptor, may be associated with periodontitis in certain ethnic groups. However relatively large variations in carriage rates of the Rare (R)-alleles among studies on any polymorphism were observed. The available studies appear under-powered and do not adequately take into account other pertinent risk factors for periodontitis. Future studies should include larger cohorts, should clearly define phenotypes and should adequately control for other risk factors. In addition to the candidate gene approach, alternative strategies need to be considered to elucidate the gene variations, which confer risk for periodontitis.

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Increased Levels of Soluble CD14 in Sera of Periodontitis Patients

Cited by 63 publications

References 35 publications

Porphyromonas gingivalis Lipopolysaccharide Upregulates Insulin Secretion From Pancreatic β Cell Line MIN6

Porphyromonas gingivalis Lipopolysaccharide Upregulates Insulin Secretion From Pancreatic β Cell Line MIN6

Estimation of sCD14 levels in saliva obtained from patients with various periodontal conditions

Identification of genetic risk factors for periodontitis and possible mechanisms of action

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