Estimation of sCD14 levels in saliva obtained from patients with various periodontal conditions

Dm, Isaza-Guzmán; Aristizábal-Cardona, D; Mc, Martínez-Pabón; Velásquez-Echeverri, H; Tobón‐Arroyave, Sergio Iván

doi:10.1111/j.1601-0825.2007.01400.x

Cited by 27 publications

(28 citation statements)

References 52 publications

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“…Following previous described methods (Isaza‐Guzmán, Aristizábal‐Cardona, Martínez‐Pabón, Velásquez‐Echeverri, & Tobón‐Arroyave, ), ~5 ml of unstimulated whole saliva were collected from each participant by spitting into sterile plastic centrifuge tube (Falcon, Corning Science Mexico SA de CV, Tamaulipas, Mexico). Saliva was collected from participants with overnight fasting (8–12 hr) and without any oral hygiene procedures.…”

Section: Methodsmentioning

confidence: 99%

Salivary levels of specialized pro‐resolving lipid mediators as indicators of periodontal health/disease status

Tobón‐Arroyave

Isaza-Guzmán

Gómez-Ortega

et al. 2019

J Clinic Periodontology

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Aim This cross‐sectional case–control study aimed to determine if salivary levels of lipoxin A4 (LXA4), protectin D1 (PD1), resolvin E1 (RvE1) and maresin 1 (MaR1) might constitute a reflection of periodontal health/disease status. Materials and methods One hundred and two periodontitis patients and 61 healthy controls were recruited. Periodontal clinical status was determined by criteria based on full‐mouth clinico‐radiographical data. Salivary concentration of the analytes was calculated by enzyme‐linked immunosorbent assay. The association between the biomarkers with disease status was assessed individually and adjusted for confounding using multivariate binary logistic regression models. Results Significantly decreased LXA4 and increased PD1/MaR1 salivary levels were detected in periodontitis patients in comparison with healthy controls. However, no significant differences were observed for RvE1 levels between clinical groups. Clinical parameters such as probing depth, clinical attachment loss and extent were negatively correlated with LXA4, positively correlated with PD1/MaR1 and not correlated with RvE1 salivary levels. Logistic regression analyses revealed a strong/independent association of LXA4, PD1 and MaR1 salivary levels regarding disease status. Interaction effects between demographic predictor variables and salivary concentration of LXA4, PD1 and MaR1 were also identified. Conclusion The results of this study demonstrated a strong/independent association between reduced LXA4 and increased PD1/MaR1 salivary levels with periodontitis suggesting an imbalance in the specialized pro‐resolving lipid mediators (SPMs) in periodontal disease.

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Section: Methodsmentioning

confidence: 99%

Salivary levels of specialized pro‐resolving lipid mediators as indicators of periodontal health/disease status

Tobón‐Arroyave

Isaza-Guzmán

Gómez-Ortega

et al. 2019

J Clinic Periodontology

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“…Interestingly, there is evidence from 2 independent studies that levels of salivary soluble CD14 (sCD14) are elevated in periodontitis [87, 91]; sCD14 mediates the action of LPS and may promote bacterial invasion of host cells in periodontitis [92, 93]. Also, although salivary sTLR2 augments IL-8 (CXCL8) production in monocytes [94], there is evidence for diminished levels of sTLR2 in periodontitis saliva [87].…”

Section: Protein Biomarkers Of Periodontitis: Candidate Protein Stmentioning

confidence: 99%

“…For example, antibodies to Aggregatibacter actinomycetemcomitans (a periodontal pathogen often associated with aggressive periodontitis) and Porphyromonas gingivalis are thought to have a key role in protective immunity in aggressive periodontitis [140] but although these antibodies are indeed found in saliva their presence is not specific to aggressive periodontitis [141, 142]. A very limited number of studies (of comparatively few patients) have directly compared levels of mediators in aggressive periodontitis and chronic periodontitis [91, 127, 143]; with the exception of one study which reported elevated cathepsin C activity in the saliva of aggressive periodontitis patients [143] none of these studies have identified biomarkers which might be potentially specific for aggressive periodontitis. Although there is a real need for larger studies, establishing specific protein biomarkers may be challenging as there is no evidence for significant differences in the immunopathogenesis of these two clinical manifestations of periodontitis [139].…”

Section: Protein Biomarkers Of Periodontitis: Candidate Protein Stmentioning

confidence: 99%

Protein Biomarkers of Periodontitis in Saliva

Taylor

2014

ISRN Inflammation

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Periodontitis is a chronic inflammatory condition of the tissues that surround and support the teeth and is initiated by inappropriate and excessive immune responses to bacteria in subgingival dental plaque leading to loss of the integrity of the periodontium, compromised tooth function, and eventually tooth loss. Periodontitis is an economically important disease as it is time-consuming and expensive to treat. Periodontitis has a worldwide prevalence of 5–15% and the prevalence of severe disease in western populations has increased in recent decades. Furthermore, periodontitis is more common in smokers, in obesity, in people with diabetes, and in heart disease patients although the pathogenic processes underpinning these links are, as yet, poorly understood. Diagnosis and monitoring of periodontitis rely on traditional clinical examinations which are inadequate to predict patient susceptibility, disease activity, and response to treatment. Studies of the immunopathogenesis of periodontitis and analysis of mediators in saliva have allowed the identification of many potentially useful biomarkers. Convenient measurement of these biomarkers using chairside analytical devices could form the basis for diagnostic tests which will aid the clinician and the patient in periodontitis management; this review will summarise this field and will identify the experimental, technical, and clinical issues that remain to be addressed before such tests can be implemented.

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“…The soluble form (sCD14), spontaneously released by these cells, circulates in the serum in high amounts (2-4 µg mL -1 ) and interacts with the LPS-binding protein (LBP) (9). The LPS/LBP/CD14 ternary complex interacts with TLRs, which leads to cell activation (10). Gingival epithelial cells, which are the first to encounter periodontal pathogens, express mostly TLRs.…”

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confidence: 99%

Salivary sCD14 as a potential biomarker of dental caries activity in adults

Prester

Protrka²,

Macan

et al. 2017

Archives of Industrial Hygiene and Toxicology

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CD14 is a co-receptor involved in the recognition of Gram-negative and Gram-positive bacteria, the latter known to cause dental caries. The aim of this study was to determine whether soluble CD14 (sCD14) in saliva was associated with caries activity and the collection method from the saliva. The study included 55 participants aged 20 to 40 years, 30 with dental caries and 25 caries-free controls. We collected 110 saliva samples in total, 55 of resting saliva and 55 of mechanically stimulated saliva. Median levels of sCD14, measured with a matrix-matched enzyme-linked immunosorbent assay (ELISA), were higher in the caries-active than in the caries-free group in either resting (203.3 vs.167.9 ng mL ; P<0.01). Hyposalivation was observed only in the caries-active group (10 and 13 % in stimulated and resting saliva, respectively). Higher salivary sCD14 levels and secretion rates were clearly associated with dental caries and resting saliva. Future studies should focus on the clinical utility of salivary sCD14 as a potential biomarker and predictor of future caries events.

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Estimation of sCD14 levels in saliva obtained from patients with various periodontal conditions

Cited by 27 publications

References 52 publications

Salivary levels of specialized pro‐resolving lipid mediators as indicators of periodontal health/disease status

Salivary levels of specialized pro‐resolving lipid mediators as indicators of periodontal health/disease status

Protein Biomarkers of Periodontitis in Saliva

Salivary sCD14 as a potential biomarker of dental caries activity in adults

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