Increased levels of oxidative stress markers in the peritoneal fluid of women with endometriosis

Polak, Grzegorz; Wertel, Iwona; Barczyński, Bartłomiej; Kwaśniewski, Wojciech; Bednarek, Wiesława; Kotarski, Jan

doi:10.1016/j.ejogrb.2012.12.043

Cited by 48 publications

(28 citation statements)

References 22 publications

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“…We and others have demonstrated the potential for an important role for oxidative stress (lipid peroxides) in the etiology of endometriosis [4; 14; 69]. In addition to cytokines, chemokines, and growth factors, there is an abundance of oxidative stress markers in the PF of women with endometriosis [14; 59; 68]. We and others have shown the presence of lipoproteins, especially LDL in the PF of women with endometriosis [46; 47; 58; 79].…”

Section: Discussionmentioning

confidence: 99%

Oxidation-sensitive nociception involved in endometriosis-associated pain

Ray¹,

Fahrmann

Mitchell

et al. 2015

Pain

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Endometriosis is a disease characterized by the growth of endometrial tissue outside the uterus and is associated with chronic pelvic pain. Peritoneal fluid (PF) of women with endometriosis is a dynamic milieu, rich in inflammatory markers and pain-inducing prostaglandins PGE2/PGF2α and lipid peroxides, and the endometriotic tissue is innervated with nociceptors. Our clinical study showed the abundance of oxidatively-modified lipoproteins in the PF of women with endometriosis and the ability of antioxidant supplementation to alleviate endometriosis-associated pain. We hypothesized that oxidatively-modified lipoproteins present in the PF are the major source of nociceptive molecules that play a key role in endometriosis-associated pain. In this study, PF obtained from women with endometriosis or control women were used for (i) the detection of lipoprotein derived oxidation-sensitive pain molecules, (ii) the ability of such molecules to induce nociception, and (iii) the ability of antioxidants to suppress this nociception. LC-MS/MS showed the generation of eicosanoids by oxidized-lipoproteins similar to that seen in the PF. The oxidatively-modified lipoproteins induced hypothermia (intra-cerebroventricular) in CD-1 mice and nociception in the Hargreaves paw-withdrawal latency assay in Sprague-Dawley rats. Antioxidants, vitamin-E and N-acetylcysteine and the NSAID, indomethacin suppressed the pain inducing ability of oxidatively-modified lipoproteins. Treatment of human endometrial cells with oxidatively-modified lipoproteins or PF from women with endometriosis showed up-regulation of similar genes belonging to the opioid and inflammatory pathways. Our finding that oxidatively-modified lipoproteins can induce nociception has a broader impact not only in the treatment of endometriosis-associated pain but also in other diseases associated with chronic pain.

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Section: Discussionmentioning

confidence: 99%

Oxidation-sensitive nociception involved in endometriosis-associated pain

Ray¹,

Fahrmann

Mitchell

et al. 2015

Pain

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“…The other factors, such as vascular endothelial growth factor (VEGF ) [7,8,9], inhibin A and B[8], anti-Müllerian hormone [10], lactoferrin [11], insulin-like growth factor-II (IGF-II) [12], hyaluronan [13], nitric oxide [9,14], leptin [15,16], 25-OH vitamin D, glucose [17], bone morphogenetic proteins (BMPs) [18,19] and interleukin 8 [20], correlate with oocyte maturation and folliculogenesis. This microenvironment may be altered by conditions, such as endometriosis [21,22,23], polycystic ovary syndrome [24] and reproductive aging [25,26,27]. Reproductive aging is marked by the decline in ovarian response, oocyte number [1,28,29,30,31] and quality [30].…”

Section: Introductionmentioning

confidence: 99%

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Hashemitabar

Bahmanzadeh

Mostafaie

et al. 2014

IJMS

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The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identiﬁcation of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20–32 years old) and older (38–42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility. The FF was analyzed by two-dimensional gel electrophoresis (2-DE). The power of two-dimensional gel electrophoresis and the identiﬁcation of proteins were exploited using matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry. Twenty three protein spots showed reproducible and significant changes in the aged compared to the young group. Of these, 19 protein spots could be identified using MALDI-TOF-TOF-MS. As a result of MASCOT search, five unique downregulated proteins were identified in the older group. These were identified as serotransferrin, hemopexin precursor, complement C3, C4 and kininogen. A number of protein markers were found that may help develop diagnostic methods of infertility.

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“…Endometriosis also possesses a malignant transformation potential to ovarian carcinoma. Recently, several studies have indicated that heme and iron, present in endometriotic cysts, damage the endometriotic cells by inducing inflammation and oxidative stress (3)(4)(5)(6)(7). Simultaneously, several genes associated with acute antioxidant response, DNA damage repair and cell cycle modulation are activated.…”

Section: Introductionmentioning

confidence: 99%