Increased levels of interleukin-6 and interleukin-10 in the peritoneal fluid of patients with endometriosis

Punnonen, Juha; Teisala, Klaus; Ranta, Hannu; Bennett, Bruce; Punnonen, R.

doi:10.1016/s0002-9378(96)70600-2

Cited by 149 publications

(78 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Unsurprisingly, therefore, elevated levels of these cytokines are observed in peritoneal fluid of women with endometriosis, suggesting their involvement in endometriosis and its symptoms (51,65). Thus, our finding that levels of these cytokines were the same in cysts and uterine horn and did not change from proestrus to estrus was surprising.…”

Section: Cytokine Involvement In Endo-induced Hyperalgesiamentioning

confidence: 66%

Endometriosis as a neurovascular condition: estrous variations in innervation, vascularization, and growth factor content of ectopic endometrial cysts in the rat

Zhang

Dmitrieva²,

Liu³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Endometriosis is a poorly understood, estradiol-dependent condition associated with severe pelvic pains and defined by vascularized endometrial growths outside the uterus. Endometriosis is produced in cycling rats by autotransplanting pieces of uterus onto abdominal arteries where they develop into cysts. The surgery induces vaginal and abdominal muscle hyperalgesia, whose severity is greatest in proestrus and nearly absent in estrus. The cysts contain growth factors and cytokines and develop their own sympathetic and sensory C-and A␦-fiber innervation. Here, we used quantitative immunostaining and protein array analyses to test the hypothesis that the innervation and growth factor/cytokine content of the cysts, but not uterine horn, contribute to proestrous-to-estrous changes in hyperalgesic severity. If so, these characteristics in the cysts, but not the uterine horn, should change with estrous stage. In cysts, the density of sympathetic (but not sensory) neurites and amounts of NGF and VEGF proteins (but not cytokines IL-1, IL-6, IL-10, or TNF-␣) were greater in proestrus than estrus. These changes were accompanied by vascular changes. Both sympathetic and sensory fibers in both stages colabeled with TrkA, indicating that changes in NGF could act on both afferent and efferent fibers. In contrast with the cysts, no changes occurred in the uterine horn between proestrus and estrus. Together, these results suggest that coordinated proestrous-to-estrous changes in innervation and vascularization of the cysts contribute to similar changes in hyperalgesic severity. The findings also encourage consideration of endometriosis as a neurovascular condition.

show abstract

Section: Cytokine Involvement In Endo-induced Hyperalgesiamentioning

confidence: 66%

Endometriosis as a neurovascular condition: estrous variations in innervation, vascularization, and growth factor content of ectopic endometrial cysts in the rat

Zhang

Dmitrieva²,

Liu³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…This may be explained by the fact that only patients with more advanced stages of endometriosis were included in this previous study. Punnonen et al (7) reported increased levels of IL-6 and IL-10 in the PF of patients with endometriosis and also did not identify any correlation between IL-6 levels and disease severity. Furthermore, Khan et al (10) did not observe any differences in IL-6 levels in the PF between patients with endometriosis and controls without the disease.…”

Section: Discussionmentioning

confidence: 93%

Circulating and peritoneal fluid interleukin-6 levels and gene expression in pelvic endometriosis

Andrade

Nácul

Santos

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. Current data are inconsistent regarding the association between interleukin-6 (IL-6), a marker of acute phase inflammation, and pelvic endometriosis. The aim of the present study was to assess IL-6 levels in serum and peritoneal fluid (PF), as well as IL-6 gene expression in adipose tissue (AT) and endometrial samples in pelvic endometriosis. A total of 30 patients with endometriosis and 18 women with a normal pelvis were enrolled in this case-control study. IL-6 levels in PF and serum were determined using a human enzyme-linked immunosorbent assay and IL-6 gene expression was evaluated using reverse transcription-quantitative polymerase chain reaction. It was observed that IL-6 levels in the PF were higher in patients with endometriosis than in the control group (P=0.047) and patients with stage III/IV endometriosis exhibited higher IL-6 levels in the PF than those with stage I/II endometriosis and the control group (P<0.001). Furthermore, a strong correlation between PF IL-6 levels and the revised American Society for Reproductive Medicine score for endometriosis severity was identified (r=0.77; P<0.001). IL-6 gene expression did not differ significantly between endometriosis and control groups in endometrial samples or in AT of both groups. The results of the current study suggest that there may be an association between IL-6 and the presence and severity of pelvic endometriosis. The source of this higher IL-6 seems not to be specifically related to regional AT.

show abstract

“…Ключевыми клетками, за счет которых ИЛ-10 оказывает свое иммуносупрес-сивное действие, являются антигенпредстав-ляющие клетки и лимфоциты. Стоит отметить, что в присутствии ИЛ-10 также формируется пул Т-регуляторных лимфоцитов, а через воз-действие на антигенпредставляющие клетки может изменяться соотношение Т-хелперов 1-го и 2-го порядков [12]. Снижение уровня данного цитокина у больных с глубоким ин-фильтративным эндометриозом также ведет к хронизации воспаления за счет неэффек-тивной работы Т-хелперов и Т-регуляторных лимфоцитов и повышенному уровню циклоок-сигеназы-2 и простагландинов в области пер-вичного поражения, что, наряду со сниженным уровнем ИЛ-2, приводит к уменьшению цито-токсической функции NK-клеток и развитию болевого синдрома.…”

Section: таблицаunclassified

Evaluation of the content and the pathogenetic role of cytokines in the peritoneal fluid in patients with deep infiltrative endometriosis

et al. 2017

View full text Add to dashboard Cite

Background. Deep infiltrative endometriosis (DIE) is characterized by the invasion of endometriosis lesions in tissues and organs to a depth of over 5 mm. In recent years, the proportion of infiltrative forms of endometriosis has been steadily increasing. The main clinical manifestations is chronic pelvic pain syndrome and infertility. A key element in the pathogenesis of deep infiltrative endometriosis is an ineffective inflammatory response.Objective. Evaluate the content and the role of pro- and anti-inflammatory cytokines, growth factors and chemokines in the pathogenesis of deep infiltrative endometriosis for pathogenetically grounded immunomodulatory therapy.Materials and methods. The present study included 120 women with deep infiltrative endometriosis. In the peritoneal fluid, using IFA determined the level of IL-33, and with the help of running cytofluometry format NEA has estimated the levels of IL-2, IL-6, IL-10, IP-10, MCP-1 and growth factors – FGF, TGF-β.Results. In the study of peritoneal fluid of patients with DIE was a significant decrease in the level of IL-2 and IL-10 6.7 times compared to the control group. The level of IL-6 was increased in 1.5 times, as well as the level of IL-33, and was awarded the data link cytokines with the severity of pain. DIE is characterized by increased levels of MCP-1 in 2 times and decrease in IP-10 1.3 times, as well as increased levels of FGF 1.5 times and reduced levels of TGF-β in 1.9 times in comparison with the control group.Conclusion. For effective treatment of DIE and to increase the duration of recurrence-free period actual and pathogenetically justified is the inclusion of a combined treatment of immunomodulatory therapy with recombinant IL-2 aimed at the elimination of immunological disorders in the pelvic cavity.

show abstract

Increased levels of interleukin-6 and interleukin-10 in the peritoneal fluid of patients with endometriosis

Cited by 149 publications

References 20 publications

Endometriosis as a neurovascular condition: estrous variations in innervation, vascularization, and growth factor content of ectopic endometrial cysts in the rat

Endometriosis as a neurovascular condition: estrous variations in innervation, vascularization, and growth factor content of ectopic endometrial cysts in the rat

Circulating and peritoneal fluid interleukin-6 levels and gene expression in pelvic endometriosis

Evaluation of the content and the pathogenetic role of cytokines in the peritoneal fluid in patients with deep infiltrative endometriosis

Contact Info

Product

Resources

About