Increased level of superoxide dismutase (SOD) activity in larvae of Chironomus ramosus (Diptera: Chironomidae) subjected to ionizing radiation

Datkhile, Kailas D.; Mukhopadhyaya, Robin; Dongre, T. K.; Nath, Bimalendu B.

doi:10.1016/j.cbpc.2008.11.003

Cited by 42 publications

(30 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Significant induction of SOD was shown in marine mussels Mytilusgalloprovincialis in the contaminated sites in the lagoon of Venice . Other studies show the increase of Cu, Zn-SOD under excessive oxidative stress and no induction of Mn-SOD in invertebrate animals (Datkhile et al, 2009). However, in the present study, the responses of SOD were highly seasonally dependent.…”

Section: A B C D E F Gcontrasting

confidence: 75%

Untitled

Falfushynska¹,

Gnatyshyna²,

Gyori³

et al. 2014

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The aim of the present study was to ascertain whether the metallothioneins (MTs) of freshwater mussels can serve as a biomarker of environmental quality in the spontaneously polluted area. Anodonta anatina mussels from an agricultural site (A), the cooling pond of a nuclear power plant (N) and a forestry as a reference site (F) in Western Ukraine were studied during spring, summer and autumn. Concentrations of copper (Cu), zinc (Zn) and cadmium (Cd) in MTs and in the tissue, MTs protein (MT-SH) level, oxidative stress indices and apoptotic activities were determined in the digestive gland. The three sites were clear distinguished by biomarkers. In the A-group, the specimens, despite higher concentrations of Cu and Zn in the tissue, had low level of MT-SH level (in two seasons) making the metal-keeping function of MTs invalid. MTs-bound metals dropped to about 2.3% of Cu, 0.4% of Zn and 9.1% of Cd in the tissue in autumn in this polluted area. Lower level of glutathione redox-index reflected the oxidative stress in this group. The N-group displayed high levels of MT-bound Cu (corresponding to up to 57.7% of the total tissue metal) and caspase-3 activity (up to 7 times higher than in the reference Fgroup), indicating specific for cooling pond pollution by Cu and thermal effects. In group F, the set of markers showed the characteristic of the reference site. An extremely low redox-index of glutathione (RI GSH: 0.45) was observed in the A-group in spring, and high levels of protein carbonyls and TBA-reactive substances in autumn in group N.Hence, multi-marker approach needed to provetoxicityassesments in mussel sexposed byspontaneous and mixed pollution.

show abstract

Section: A B C D E F Gcontrasting

confidence: 75%

Untitled

Falfushynska¹,

Gnatyshyna²,

Gyori³

et al. 2014

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…The total superoxide dismutase (T-SOD) activity was measured following the method of Zhang et al (2008) [48] with slight modifications and using head kidney and spleen homogenates instead of hepatic homogenates. To distinguish SOD isoforms, we used the sensitivity of CuZnSOD to cyanide; in contrast, MnSOD is unaffected according to the method described in a previous study [49]. Glutathione peroxidase (GPx) activities were assayed as previously described [48].…”

Section: Biochemical Analysismentioning

confidence: 99%

“…The protein content was determined according to a previous study [52] and was used to calculate the antioxidant enzyme activities and the GSH content of the fish. Generally, with the exception of SOD, in which 1 unit of activity was considered to be half of the inhibition of absorbance compared to a tube lacking the enzyme according to the method described by Datkhile et al [53], in the enzyme assays 1 unit of activity was considered to be the amount of enzyme required to transform 1 mmol of substrate min À1 .…”

Section: Biochemical Analysismentioning

confidence: 99%

Dietary myo-inositol modulates immunity through antioxidant activity and the Nrf2 and E2F4/cyclin signalling factors in the head kidney and spleen following infection of juvenile fish with Aeromonas hydrophila

Jiang

Liu

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Different expressions of SOD and CAT isoforms have been shown in control and stressed larvae of Hyphantria cunea (Kim et al 2010) and Protaetia brevitarsis (Kim et al 2008). Also, the expression of several SOD isozymes and significantly increased intensity of two bands, as well as higher activity detected on the CAT zymogram, have been recorded in larvae of Chironomus ramosus exposed to a stressful condition (Datkhile et al 2009). Dynamic modulation in the expression of a particular isoform of any of these antioxidative enzymes can be more beneficial for it maintains redox homeostasis than changes in its total activity, in the state of stress exerted by environmental agents.…”

Section: Discussionmentioning

confidence: 94%

Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Mrdaković

Ilijin

Vlahović

et al. 2015

Environ Sci Pollut Res

View full text Add to dashboard Cite

This study aimed to examine the effects of ubiquitous polycyclic aromatic hydrocarbon fluoranthene, supplemented to an artificial diet, on the fitness-related traits and activity of midgut antioxidative enzymes-superoxide dismutase (SOD) and catalase (CAT), and expression of their isoforms in the fifth-instar gypsy moth Lymantria dispar L. Prolonged duration of development and reduced weight and relative growth rate were recorded in larvae reared on the diets supplemented with different concentrations of fluoranthene. SOD and CAT activities were significantly higher in the midguts of fluoranthene-treated larvae, compared to that of the control group. Different expression patterns were detected for SOD as well as for CAT isoforms, depending on the supplemented concentration of fluoranthene. Obtained results suggest that the activity of these enzymes in gypsy moth larvae may be used as biomarkers for assessing pollution, even at low concentrations of the pollutant.

show abstract

Increased level of superoxide dismutase (SOD) activity in larvae of Chironomus ramosus (Diptera: Chironomidae) subjected to ionizing radiation

Cited by 42 publications

References 36 publications

Untitled

Untitled

Dietary myo-inositol modulates immunity through antioxidant activity and the Nrf2 and E2F4/cyclin signalling factors in the head kidney and spleen following infection of juvenile fish with Aeromonas hydrophila

Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Contact Info

Product

Resources

About