Increased invasion activity of endometrial stromal cells and elevated expression of matrix metalloproteinase messenger RNA in the uterine tissues of mice with experimentally induced adenomyosis

Matsuda, Manabu; Sasabe, Haruko; Adachi, Yuji; Suzuki, Tatsuya; Mori, Taketoshi

doi:10.1067/mob.2001.117967

Cited by 25 publications

(25 citation statements)

References 20 publications

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“…Recent results have shown that concentrations of cathepsin D in the peritoneal fluid are elevated in patients affected by endometriosis [14]. Further, Matsuda et al [15] have shown that in adenomyosis invasion of endometrial stromal cells into extracellular matrix is associated with an elevation of matrix metalloproteinase 14 (MMP 14) in vitro, and that MMP 14 inhibitor is capable of suppressing the invasion, indicating increased proteolytic activity of ectopic endometrial tissue.…”

Section: Discussionmentioning

confidence: 99%

Intrinsic Form of Ureteral Endometriosis Causing Ureteral Obstruction and Partial Loss of Kidney Function

Horn¹,

Minh

Stolzenburg

2004

Urol Int

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Introduction: Urinary tract endometriosis is rare and occurs in about 1% of all endometriotic lesions. About 30% of patients suffer from reduced kidney function at the time of diagnosis. Case Report: A 49-year-old woman presented with a history of abdominal hysterectomy without adnexae because of uterus myomatosus without any signs of endometriosis 6 years before. Now she complained of dysuria and intermittent left loin pain. Sonography and retrograde pyelography demonstrated incomplete ureteric obstruction and scintigraphy revealed a partial loss of kidney function. Intraoperatively frozen section histology and final histologic examination demonstrated a tumorlike intrinsic form of ureteral endometriosis engulfing the left ureter. The patient was treated with uretero-ureterostomy and danazole for preventing recurrence. She is well 28 months postoperatively. Conclusions: In the extrinsic form (about 75% of all cases), ureteral endometriosis is localised to the adventitia or surrounding connective tissue of the ureter, whereas the intrinsic form of ureteral endometriosis is very rare and more often needs aggressive surgery.

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Section: Discussionmentioning

confidence: 99%

Intrinsic Form of Ureteral Endometriosis Causing Ureteral Obstruction and Partial Loss of Kidney Function

Horn¹,

Minh

Stolzenburg

2004

Urol Int

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“…MMPs, recently, have been implicated in the invasion and development of adenomyosis. MMP inhibitor may suppress the invasiveness of stromal cells with high ECM-degrading activity in mouse adenomyotic tissues [18,19] . Furthermore, it has been reported that MMPs appeared to be over-expressed in human adenomyotic uterine cavity compared with normal uterus [20] , but few researches have probed into the mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinase-2 and -9 Expression Correlated with Angiogenesis in Human Adenomyosis

Tian

2006

Gynecol Obstet Invest

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Background: Adenomyosis is an important cause of dysmenorrhea and infertility for women all over the world, however, the pathogenesis has not been completely elucidated. The purpose of the study was to investigate the role of matrix metalloproteinases (MMPs) and their relation to angiogenesis in human adenomyosis. Methods: Adenomyotic endometrial specimens were removed by hysterectomy from 68 women with adenomyosis. The control group consisted of 26 normal endometrial specimens. Immunohistochemistry was used to demonstrate expression of MMP-2, -9, vascular endothelial growth factor (VEGF) and microvessel density (MVD). Staining intensity was analyzed by computerized image analysis system. Results: In both eutopic and ectopic endometrium of adenomyosis, the expression of MMP-2, -9 as well as VEGF was significantly greater than in normal endometrium (p < 0.05). MVD was higher in ectopic endometrium than eutopic endometrium with or without adenomyosis (p < 0.05). In adenomyosis, a positive correlation was observed between VEGF expression and MMP-2 (p < 0.001, r = 0.583) as well as MMP-9 expression (p = 0.002,r = 0.490). A positive correlation was also found between MVD and MMP-2 (p < 0.001,r = 0.589) or MMP-9 expression (p < 0.001,r = 0.589). Conclusion: Our results indicate that the elevation of MMP-2, -9 expression may have an important role in the development of adenomyosis, probably through contributing to invasion of endometrial tissues into the myometrium and angiogenesis in adenomyotic implants.

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“…The invasive activity of the endometrial stromal cells into various kinds of extracellular matrix components was examined in experimentally induced adenomyosis in SHN mice, and it was suggested that the increased matrix metalloproteinase-14 may play an important role in the invasion of endometrial tissues into the myometrium (Matsuda et al 2001). Laminins and fibronectins are important glycoprotein components of the extracellular matrix and basement membranes, and are critically involved in cell differentiation in early development and in tissue formation and maintenance of mature tissues (Romberger 1997, Sasaki et al 2004.…”

Section: Controlmentioning

confidence: 99%

The effects of tamoxifen and estradiol on myometrial differentiation and organization during early uterine development in the CD1 mouse

Mehasseb

Bell²,

Habiba³

2009

Reproduction

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We used a neonatal mouse model to examine the histogenesis of uterine adenomyosis, and to test whether adenomyosis is due to an abnormality in myometrial differentiation, or in extracellular matrix proteins expression. We also studied the effects of tamoxifen and estradiol on uterine development, myometrial differentiation, and organization. Female CD1 pups were treated with oral tamoxifen (1 mg/kg) (nZ27) or estradiol (0.1 mg/kg) (nZ24) from age 1 to 5 days. Uteri from control (nZ27) and treated mice were obtained on days 2, 5, 10, 15, and 42 of age. We examined the sections histologically, using image analysis and immunohistochemistry for a-smooth muscle actin (a-SMA), desmin, vimentin, laminin, fibronectin, and estrogen receptor-a. Following tamoxifen exposure, all uteri showed adenomyosis by 6 weeks of age (seen as early as day 10). The inner myometrium showed thinning, lack of continuity, disorganization, and bundling. a-SMA expression was normal. Desmin expression normally showed a wave of maturation that was absent in tamoxifen-treated mice. In the estradiol group, adenomyosis was not observed. All uterine layers were normally developed, but hypertrophied. The inner myometrium retained its circular arrangement. There was no difference in the localization of laminin or fibronectin between groups (laminin expression was reduced in the tamoxifen treated uteri). Vimentin could not be detected in all groups. Our results suggest that the development of the inner myometrium is particularly sensitive to estrogen antagonism, and can be affected by steroid receptors modulation. Disruption of the inner myometrium may play a role in the development of uterine adenomyosis.

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Increased invasion activity of endometrial stromal cells and elevated expression of matrix metalloproteinase messenger RNA in the uterine tissues of mice with experimentally induced adenomyosis

Cited by 25 publications

References 20 publications

Intrinsic Form of Ureteral Endometriosis Causing Ureteral Obstruction and Partial Loss of Kidney Function

Intrinsic Form of Ureteral Endometriosis Causing Ureteral Obstruction and Partial Loss of Kidney Function

Matrix Metalloproteinase-2 and -9 Expression Correlated with Angiogenesis in Human Adenomyosis

The effects of tamoxifen and estradiol on myometrial differentiation and organization during early uterine development in the CD1 mouse

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