Matrix Metalloproteinase-2 and -9 Expression Correlated with Angiogenesis in Human Adenomyosis

Tian, Li; Li, Yunguang; Pu, Demin

doi:10.1159/000094426

Cited by 75 publications

(75 citation statements)

References 48 publications

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“…In healthy women, MMP enzyme expression alterations have been identified in the endometrium according to the menstrual cycle phases and have been higher during the secretory cycle phase than during the proliferative phase. In contrast to this finding, MMP enzyme expression in adenomyosis was highest during the proliferative phase and underwent insignificant alterations during the menstrual cycle [12,13] . In this study, a statistically significantly higher blood serum concentration of RCAS1 has been observed in adenomyosis and leiomyoma than in patients with uterine leiomyoma alone in the proliferative cycle phase (p !…”

Section: Discussioncontrasting

confidence: 80%

“…The invasion of endometrial cells is determined by their capacity to secrete matrix metalloproteinase (MMP) enzymes. It has been shown that the expression of MMP enzymes increases in patients with adenomyosis in comparison with healthy women [12,13] . The maintenance of ectopic endometrial cells within the myometrium can be enabled not only by their invasiveness, but also by their ability to regulate the activity of immune system cells.…”

Section: Introductionmentioning

confidence: 99%

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Alterations in RCAS1 Serum Concentration Levels during Menstrual Cycle in Patients with Uterine Leiomyoma and Lack of Analogical Changes in Adenomyosis

Wicherek¹

2008

Gynecol Obstet Invest

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Introduction: The selective suppression phenomenon of the cytotoxic immune response occurs in the endometrium at the beginning of decidualization. This process seems to be associated with an increase in the endometrial expression of proteins such as RCAS1 that are involved in the suppression of immune cell activity. The aim of the present study was to evaluate alterations in the RCAS1 blood serum concentration levels in women with uterine leiomyoma over the course of the different menstrual cycle phases and to compare these levels with those found in patients suffering from adenomyosis. Material and Methods: The sRCAS1 blood serum concentration level was determined for 87 patients, including 38 patients with both adenomyosis and uterine leiomyoma, and 49 suffering from leiomyomatosis alone. Results: Fluctuations in sRCAS1 blood serum concentration levels correlating with the menstrual cycle phases were demonstrated in patients suffering from uterine leiomyoma alone. The highest level of sRCAS1 concentration was found during the secretory cycle phase and the lowest during the proliferative cycle phase. However, no such fluctuations correlating with menstrual cycle phases were observed in patients suffering from both adenomyosis and leiomyoma. In fact, the level of sRCAS1 blood serum concentration in patients with adenomyosis remained almost constant. Patients with adenomyosis and leiomyoma were characterized by statistically significantly higher blood serum sRCAS1 levels during the proliferative cycle phase in comparison with the sRCAS1 blood serum levels in patients with leiomyoma alone. Conclusion:The lack of alterations in the sRCAS1 blood serum concentration levels observed in patients with adenomyosis may favor the development of the condition.

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Section: Discussioncontrasting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Alterations in RCAS1 Serum Concentration Levels during Menstrual Cycle in Patients with Uterine Leiomyoma and Lack of Analogical Changes in Adenomyosis

Wicherek¹

2008

Gynecol Obstet Invest

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“…Numerous studies have documented that MMP-9 aids angiogenesis through extracellular matrix degradation and release of growth factors (34). Similarly, a positive correlation between the expression of cathepsin B and angiogenesis has been reported (35).…”

Section: Downregulation Of Cathepsin B and Mmp-9 Through Rna Interfermentioning

confidence: 84%

RNAi-mediated abrogation of cathepsin B and MMP-9 gene expression in a malignant meningioma cell line leads to decreased tumor growth, invasion and angiogenesis

et al. 2007

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Abstract. Malignant meningiomas are highly aggressive and frequently recur after surgical resection of the tumor. Earlier studies have reported that the cysteine protease cathepsin B and the matrix metalloproteinase MMP-9 play important roles in tumor progression. In the present study, we made an attempt to evaluate the roles of these proteases in the malignant meningioma tumor microenvironment and determined the effectiveness of using single or bicistronic siRNA constructs for cathepsin B and MMP-9, in both in vitro and in vivo models. Transfection of a plasmid vector expressing doublestranded RNA for cathepsin B and MMP-9 significantly inhibited mRNA and protein levels of cathepsin B and MMP-9. The migration and invasion of meningioma cells were decreased after treatment with single or bicistronic siRNA constructs for cathepsin B and MMP-9 compared to controls and vector controls. Inhibition of angiogenesis was observed when the cells were transfected with single or bicistronic constructs for cathepsin B and MMP-9, when compared to controls or empty vector controls. Our study revealed that abrogation of cathepsin B and MMP-9 expression decreased the activation of major proteins involved in MAP kinase and PI3 kinase pathways indicating that targeting these proteases may hinder intracellular signaling and thus decrease cell survival and proliferation in malignant meningiomas. In addition to the in vitro evidence, we observed a significant regression of pre-established orthotopic tumors after treatment with RNAi plasmid vectors targeting cathepsin B and MMP-9. Furthermore, these observations demonstrate that the simultaneous RNAi-mediated targeting of cathepsin B and MMP-9 has potential application for the treatment of human meningiomas.

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“…-интерлейкина-1, -6, -11 (IL-I, -6, -11) [45,46]; -лейкемияингибирующего фактора (LIF) [47]; -сосудисто-эндотелиального фактора роста (VEGF) [48]; -гепаринсвязывающего эпидермального фак-тора роста (ГС-ЭФР) [49]; -матриксных металлопротеиназ (ММР) и их тканевых ингибиторов (ТIMP) [50]; -интегринов [46,47]; -селектинов [51,52]; -гликоделина A [53]; -кадгеринов [45]; -муцинов [45]; -простагландинов [54]; -белка Ki-67 [55,56]. Перечисленные молекулярные маркеры могут определяться в крови, секрете эндометрия или в са-мой ткани эндометрия, полученной при его биопсии.…”

Section: проблемы репродукции 5 2016unclassified

Modern approaches to endometrial receptivity assessment (a review)

Krasnopolskaya¹,

Nazarenko²,

Ershova³

2016

Probl. reprod.

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Согласно существующим представлениям, все причины безуспешности попыток ЭКО, в которых удается выполнить перенос полученных in vitro эм-брионов, могут быть связаны либо с их неспособно-стью к внутриматочной нидации (эмбриональный фактор), либо со снижением рецептивности эндоме-трия (эндометриальный фактор) [1,2]. Необходи-мость совершенствования эндометриального факто-ра программ ЭКО определяется тем, что при исполь-зовании морфологически хороших эмбрионов, по-лученных при оплодотворении донорских ооцитов в программах ЭКО-ОД, только ненамного повышает частоту успешных имплантаций в сравнении со стандартным ЭКО (где качество переносимых эм-брионов может быть не столь хорошим). Это можно видеть из отчета РАРЧ за 2013 г. (опубликован в 2015 г.), согласно которому частота наступления бе-ременности на перенос эмбрионов (ЧНБ пэ ) в про-грамме ЭКО-ОД превысила лишь на 5,3% аналогич-ный показатель в стандартной программе ЭКО (43,8% против 38,5%) [3]. Данный факт указывает на то, что резерв для дальнейшего повышения эффек-

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Matrix Metalloproteinase-2 and -9 Expression Correlated with Angiogenesis in Human Adenomyosis

Cited by 75 publications

References 48 publications

Alterations in RCAS1 Serum Concentration Levels during Menstrual Cycle in Patients with Uterine Leiomyoma and Lack of Analogical Changes in Adenomyosis

Alterations in RCAS1 Serum Concentration Levels during Menstrual Cycle in Patients with Uterine Leiomyoma and Lack of Analogical Changes in Adenomyosis

RNAi-mediated abrogation of cathepsin B and MMP-9 gene expression in a malignant meningioma cell line leads to decreased tumor growth, invasion and angiogenesis

Modern approaches to endometrial receptivity assessment (a review)

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