Increased H2 production by immobilized microorganisms

Kumar, Ashok; Jain, Sweta; Sharma, Charu; Joshi, Akshay; Kalia, Vipin Chandra

doi:10.1007/bf00704638

Cited by 76 publications

(29 citation statements)

References 9 publications

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“…B. licheniformis and Bacillus spp. along with other bacterial cultures have resulted in a H 2 yield in the range of 1.5-1.65 mol/mol glucose [10,28]. PHAs may generally account up to 90% of the dry cell weight (DCW) of the microbes [29].…”

Section: Polyhydroxybutyrate Productionmentioning

confidence: 99%

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

2011

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Metabolic activities of four Bacillus strains to transform glucose into hydrogen (H 2 ) and polyhydroxybutyrate (PHB) in two stages were investigated in this study. Under batch culture conditions, Bacillus thuringiensis EGU45 and Bacillus cereus EGU44 evolved 1.67-1.92 mol H 2 /mol glucose, respectively during the initial 3 days of incubation at 37°C. In the next 2 days, the residual glucose solutions along with B. thuringiensis EGU45 shaken at 200 rpm was found to produce PHB yield of 11.3% of dry cell mass. This is the first report among the non-photosynthetic microbes, where the Bacillus spp.-B. thuringiensis and B. cereus strains have been shown to produce H 2 and PHB in same medium under different conditions.

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Section: Polyhydroxybutyrate Productionmentioning

confidence: 99%

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

2011

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“…It may thus serve as a warning sign because such conditions may even have an adverse effect on growth and activity of H 2 producing microbes. Immobilized whole cell technique leads to high reaction rates and thus represents an efficient approach [171] to biocatalysis for carrying out several biochemical reactions including H 2 production [89,133,140,155,190,195,207] and CH 4 production [66]. Most of the solid matrices used for the immobilization of the whole cells are synthetic polymers or inorganic materials.…”

Section: Conditions Affecting Biological Hydrogen Productionmentioning

confidence: 99%

Microbial diversity and genomics in aid of bioenergy

Kalia

Purohit

2008

J Ind Microbiol Biotechnol

102

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In view of the realization that fossil fuels reserves are limited, various options of generating energy are being explored. Biological methods for producing fuels such as ethanol, diesel, hydrogen (H2), methane, etc. have the potential to provide a sustainable energy system for the society. Biological H2 production appears to be the most promising as it is non-polluting and can be produced from water and biological wastes. The major limiting factors are low yields, lack of industrially robust organisms, and high cost of feed. Actually, H2 yields are lower than theoretically possible yields of 4 mol/mol of glucose because of the associated fermentation products such as lactic acid, propionic acid and ethanol. The efficiency of energy production can be improved by screening microbial diversity and easily fermentable feed materials. Biowastes can serve as feed for H2 production through a set of microbial consortia: (1) hydrolytic bacteria, (2) H2 producers (dark fermentative and photosynthetic). The efficiency of the bioconversion process may be enhanced further by the production of value added chemicals such as polydroxyalkanoate and anaerobic digestion. Discovery of enormous microbial diversity and sequencing of a wide range of organisms may enable us to realize genetic variability, identify organisms with natural ability to acquire and transmit genes. Such organisms can be exploited through genome shuffling for transgenic expression and efficient generation of clean fuel and other diverse biotechnological applications.

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“…In practice, a fraction of the substrate is used for biomass production and other metabolic products are also produced, resulting in a lower hydrogen yield. Hydrogen yields by pure or mixed cultures have been reported to range from 0.37 to 2.0 mole-H 2 /moleglucose (Kataoka et al, 1997 andKumar et al, 1995). Considering the high theoretical yields, several researchers have begun exploring approaches to increase hydrogen production.…”

Section: Introductionmentioning

confidence: 99%

“…Several researcher have used physical methods to increase hydrogen yields by applying vacuum to the headspace of a bioreactor (Kataoka, et al, 1997), by sparging the biogas with nitrogen gas , by immobilizing cells (Kumar et al, 1995), by vigorous stirring to allow the dissolved hydrogen to escape to the gas phase (Lamed et al, 1988), or by using γ-Alumina, an activated alumina used as desiccant in chemical process industries, to adsorb volatile acids (Liang et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Production by Anaerobic Microbial Communities Exposed to Repeated Heat Treatments

Duangmanee¹,

Padmasiri²,

Simmons³

et al. 2002

proc water environ fed

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Biological hydrogen production by anaerobic mixed communities was studied in batch systems and in continuous-flow bioreactors using sucrose as the substrate. The systems were seeded with anaerobically digested municipal biosolids that had been heat treated at 100°C for 15 minutes. During operation, repeated heat treatments of the biomass in the reactors at 90°C for 20 minutes were performed. Results indicated that both initial heat treatment of the inoculum and repeated heat treatments of the biomass during operation promoted hydrogen production by eliminating non-spore forming hydrogen consuming microorganisms and by selecting for hydrogen producing spore forming bacteria. An operational pH of 5.5 was shown to be optimal for hydrogen production. The conversion efficiency and hydrogen yield were 0.0892 L-H 2 /g-COD and 1.5291 mole of H 2 /mole of sucrose, respectively. Terminal restriction fragment length polymorphism (T-RFLP) analysis showed that Clostridium and Bacillus species were dominant populations in the bioreactors. A positive correlation was observed between the total abundance of Clostridium species and hydrogen production during part of an operational run.

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Increased H2 production by immobilized microorganisms

Cited by 76 publications

References 9 publications

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

Hydrogen and Polyhydroxybutyrate Producing Abilities of Bacillus spp. From Glucose in Two Stage System

Microbial diversity and genomics in aid of bioenergy

Hydrogen Production by Anaerobic Microbial Communities Exposed to Repeated Heat Treatments

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