Increased expression of SUMO1P3 predicts poor prognosis and promotes tumor growth and metastasis in bladder cancer

Abstract

Bladder cancer is one of the most common malignancies worldwide. Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play crucial roles in diverse biological processes. The pseudogene-expressed lncRNA is one major type of lncRNA family. Small ubiquitin-like modifier (SUMO) 1 pseudogene 3, (SUMO1P3) is a novel indentified lncRNA that was previously reported to be up-regulated in gastric cancer. However, we know nothing about the biological function and underlying mechanism of SUMO1P3 in tumor. Furthermore, the relationship between SUMO1P3 and bladder cancer is completely unknown. We hypothesized that SUMO1P3 also have roles in bladder cancer.In this study, we found that SUMO1P3 was significantly up-regulated in bladder cancer tissues compared with paired-adjacent nontumorous tissues in a cohort of 55 bladder cancer patients. Moreover, up-regulated SUMO1P3 expression was positively correlated with greater histological grade (P<0.05) and advanced TNM stage (P<0.05). Furthermore, we found cell proliferation / migration inhibition and apoptosis induction were also observed in SUMO1P3 siRNA-transfected bladder cancer cells. Our data suggest that SUMO1P3 plays oncogenic roles in bladder cancer and can be used as a potential prognostic and therapeutic target.

Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells

“…Recently, the further comprehensive mechanism between H19 and BCa was reported in continuance3435. Our previous researches also identified several lncRNAs, like PVT1 and SUMO1P3, which play oncogenic roles in BCa3637. Therefore, the identification of BCa-associated lncRNAs may provide an opportunity to reveal the mechanism network of cancer pathogenesis in BCa for further targeted treatment.…”

“…Both of the two experiments were performed as previously reported in Zhan et al 37. The assays were performed in triplicate and the each experiments were repeated three times.…”

SPRY4-IT1: A novel oncogenic long non-coding RNA in human cancers

“…As expected, knockdown of SPRY4-IT1 decreased the proliferation and migration in bladder cancer cells. 22 …”

Long non-coding RNA HOXA transcript at the distal tip as a biomarker for gastric cancer

“…Previously, multiple studies reported that in different types of cancer there was abnormal expression of lncRNA, indicating that the same lncRNA may serve different functions in different types of cancer. For instance, a lncRNA named SPRY4 intronic transcript 1 (SPRY4-IT1) was reported to be overexpressed in melanoma, renal cell carcinoma, esophageal squamous cell carcinoma, breast cancer, bladder cancer and glioma, and associated with poor prognosis and promotion of tumor growth (24)(25)(26)(27)(28)(29). Certain studies also demonstrated that SPRY4-IT1 expression was decreased in non-small-cell lung cancer and gastric cancer, and acted with significant antitumor function in these two types of cancer (30,31).…”