Increased expression of MUC5AC and MUC5B promoting bacterial biofilm formation in chronic rhinosinusitis patients

Mao, Yongjun; Chen, Haihong; Wang, Bo; Liu, Xiang; Xiong, Gaoyun

doi:10.1016/j.anl.2014.12.004

Cited by 18 publications

(15 citation statements)

References 26 publications

(17 reference statements)

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“…An alternative argument for a role of biofilm formation in CRS suggests that the first point of formation is binding to mucin. MUC gene expression levels were significantly elevated in CRS subjects who had biofilm formation in comparison to those who did not (99). Importantly, it remains to be seen whether biofilms in CRS directly drive disease and an aberrant inflammatory response or whether CRS disease processes drive the inflammatory response, epithelial barrier breakdown, and increased MUC expression in parallel.…”

Section: Role Of Bacteria In Crsmentioning

confidence: 98%

Chronic Rhinosinusitis and the Evolving Understanding of Microbial Ecology in Chronic Inflammatory Mucosal Disease

et al. 2017

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Chronic rhinosinusitis (CRS) encompasses a heterogeneous group of debilitating chronic inflammatory sinonasal diseases. Despite considerable research, the etiology of CRS remains poorly understood, and debate on potential roles of microbial communities is unresolved. Modern culture-independent (molecular) techniques have vastly improved our understanding of the microbiology of the human body. Recent studies that better capture the full complexity of the microbial communities associated with CRS reintroduce the possible importance of the microbiota either as a direct driver of disease or as being potentially involved in its exacerbation. This review presents a comprehensive discussion of the current understanding of bacterial, fungal, and viral associations with CRS, with a specific focus on the transition to the new perspective offered in recent years by modern technology in microbiological research. Clinical implications of this new perspective, including the role of antimicrobials, are discussed in depth. While principally framed within the context of CRS, this discussion also provides an analogue for reframing our understanding of many similarly complex and poorly understood chronic inflammatory diseases for which roles of microbes have been suggested but specific mechanisms of disease remain unclear. Finally, further technological advancements on the horizon, and current pressing questions for CRS microbiological research, are considered.

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Section: Role Of Bacteria In Crsmentioning

confidence: 98%

Chronic Rhinosinusitis and the Evolving Understanding of Microbial Ecology in Chronic Inflammatory Mucosal Disease

et al. 2017

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“…In chronic rhinosinusitis, thick discharge or rhinorrhea due to an increase of mucin secretion is a troublesome symptom . Given our findings in a previous study that statins suppress MUC5AC expression, we thought that the use of statins might decrease the prevalence of chronic rhinosinusitis .…”

Section: Discussionmentioning

confidence: 77%

Effectiveness of atorvastatin in suppressing MUC5AC gene expression in human airway epithelial cells

Lee

Song

Hwang

et al. 2016

Int Forum Allergy Rhinol

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“…6a-d). Inflammatory Reactions MUC5AC and MUC5B are major components of respiratory secretion in CRS and play important roles in the pathogenesis of sinus hypersecretion in this chronic disease [25]. Accordingly, we detected the expressions of MUC5AC and MUC5B in NPs.…”

Section: T-cell Subpopulations In Npsmentioning

confidence: 95%

Efficacy of Budesonide Nasal Spray on Neutrophilic Chronic Rhinosinusitis with Nasal Polyps: A Combined Clinical and Experimental Study

Lin

Lan

Dai

et al. 2020

Int Arch Allergy Immunol

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Background: Neutrophilic chronic rhinosinusitis with nasal polyps (CRSwNP) occur predominantly in Asian subjects. Appropriate treatments for this endotype have not been elucidated. This study aimed to evaluate the efficacy of budesonide nasal spray on neutrophilic CRSwNP. Materials and Methods: Fifteen neutrophilic CRSwNP patients were included, and then they received budesonide nasal spray treatment for 3 months. Biopsies of nasal polyps (NPs) were obtained from these subjects. Their clinical indexes were scored using Visual Analog Scale (VAS), Sino-Nasal Outcome Test (SNOT)-22, and Endoscopic Appearances (EAs). Histological analyses were used to assess numbers of neutrophils, goblet cells, and submucosal gland cells in NPs. Percentages of CD8⁺ T cells and CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs) were evaluated using flow cytometry. Mucin 5AC (MUC5AC), MUC5B, myeloperoxidase (MPO), interferon (IFN)-γ, and interleukin (IL)-1β and their mRNAs were also examined. After that, we cultured NP tissues in vitro and evaluated the abovementioned inflammatory parameters before and after the administration of budesonide. Results: Budesonide nasal spray did not improve clinical evaluations including VAS, SNOT-22, and EA scores. Numbers of neutrophils and goblet cells, the score of submucosal gland cells, percentages of CD8⁺ T cells and Tregs, MUC5AC, MUC5B, MPO, IFN-γ, and IL-1β and their mRNAs were not decreased in NPs after the budesonide treatment. Furthermore, the administration of budesonide into NP cultures also did not reduce their levels in comparison with those before the treatment. Conclusion: These findings demonstrate that budesonide treatment may not alleviate the inflammatory condition in neutrophilic CRSwNP.

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Increased expression of MUC5AC and MUC5B promoting bacterial biofilm formation in chronic rhinosinusitis patients

Cited by 18 publications

References 26 publications

Chronic Rhinosinusitis and the Evolving Understanding of Microbial Ecology in Chronic Inflammatory Mucosal Disease

Chronic Rhinosinusitis and the Evolving Understanding of Microbial Ecology in Chronic Inflammatory Mucosal Disease

Effectiveness of atorvastatin in suppressing MUC5AC gene expression in human airway epithelial cells

Efficacy of Budesonide Nasal Spray on Neutrophilic Chronic Rhinosinusitis with Nasal Polyps: A Combined Clinical and Experimental Study

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