Increased expression of MMP9 is correlated with poor prognosis of nasopharyngeal carcinoma

Abstract

IntroductionThe aim of the present study was to analyze the expression of matrix metalloproteinase 9 (MMP9) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including the survival of patients with NPC.MethodsUsing real-time PCR, we detected the mRNA expression of MMP9 in normal nasopharyngeal tissues and nasopharyngeal carcinoma (NPC) tissues. Using immunohistochemistry analysis, we analyzed MMP9 protein expression in clinicopathologically characterized 164 NPC cases (116 male and 48 female) with age ranging from 17 to 80 years (median = 48.4 years) and 32 normal nasopharyngeal tissues. Cases with greater than or equal to 6 and less than 6 of the score value of cytoplasmic MMP9 immunostaining were regarded as high expression and low expression, respectively. The relationship between the expression levels of MMP9 and clinical features was analyzed.ResultsThe expression level of MMP9 mRNA was markedly greater in NPC tissues than that in the nasopharyngeal tissues. Immunohistochemical analysis revealed that the protein expression of MMP9 detected in NPC tissues was higher than that in the nasopharyngeal tissues (P = 0.004). In addition, high levels of MMP9 protein were positively correlated with the status of lymph node metastasis (N classification) (P = 0.002) and clinical stage (P < 0.001) of NPC patients. Patients with higher MMP9 expression had a significantly shorter overall survival time than did patients with low MMP9 expression. Multivariate analysis suggested that the level of MMP9 expression was an independent prognostic indicator (P = 0.008) for the survival of patients with NPC.ConclusionHigh level of MMP9 expression is a potential unfavorable prognostic factor for patients with NPC.

Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

“…Langers et al (Langers et al, 2012) have found that high expression of MMP-2 and MMP-9 in the mucosa of colon cancer patients is associated with a 5-year mean survival (Grigioni et al, 1994;Sier et al, 1996). In this study, we found similar results regarding MMP-2 and MMP-9 expression in EGISTs, i.e., the expression of MMP-2 and MMP-9 was associated with tumor progression, which is consistent with previous findings (Grigioni et al, 1994;Sier et al, 1996;Zeng et al, 1996;Ring et al, 1997;Yukawa et al, 2001;Zhang et al, 2003;Liu et al, 2010;Langers et al, 2012). However, our current study did not show the underlying molecular mechanisms responsible for upregulation of MMP-2 and MMP-9 in EGISTs or how MMP-2 and MMP-9 proteins promote EGIST progression.…”

miRNA-491-5p and GIT1 Serve as Modulators and Biomarkers for Oral Squamous Cell Carcinoma Invasion and Metastasis

“…Those data suggested that downregulation of GIT1 by miR-491-5p could suppress migration, invasion, and metastasis of OSCC cells through degradation of paxillin and reduced focal adhesion signaling, including FAK and ERK1/2 activation. miR-491-5p targets GIT1 to regulate expression level and activity of MMP2/9 via EGFR/ERK1/2 signaling pathway MMP2/9 have been implicated in tumor cell invasion and metastasis (31,32). We observed that overexpression of miR-491-5p or knockdown of GIT1 decreased MMP2/9 mRNA (Fig.…”

Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression

“…In many types of tumors, the expression level of MMP-9 is markedly higher than in normal tissues [34,35] . Moreover, patients with higher MMP-9 expression have a significantly shorter overall survival time than patients with low MMP-9 expression [36] . In our study, the observed inhibitory effects of icaritin on the expression of MMP-2 and MMP-9 in SaOS 2 cells suggested that icaritin may modulate the inva- (Figure 3-5).…”