Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

Wang, Chao; Ma, Haiying; Jin, Meishan; Zou, Yabin; Teng, Yongliang; Tian, Zhuang; Wang, Haiying; Wang, Yinping; Duan, Xiumei

doi:10.7314/apjcp.2014.15.10.4187

Cited by 11 publications

(8 citation statements)

References 27 publications

(32 reference statements)

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“…The expression and activity of MMPs against matrix macromolecules have been associated with the development of malignant phenotypes and the promotion of cell invasiveness and metastasis. MMP-2 and MMP-9 were two major MMPs mediating the degradation of the ECM (Wang et al, 2014). In our study, PPSS treatment not only reduced the protein expression but also suppressed the enzymatic activity of MMP-2 and MMP-9.…”

Section: Discussionsupporting

confidence: 59%

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9

He¹,

Zheng²,

Sun³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Tumor metastases are the main reasons for oncotherapy failure. Paris polyphylla (Chinese name: Chonglou) has traditionally been used for its anti-cancer actions. In this article, we focus on the regulation of human lung cancer A549 cell metastases and invasion by Paris polyphylla steroidal saponins (PPSS). Materials and Methods: Cell viability was evaluated in A549 cells by MTT assay. Effects of PPSS on invasion and migration were investigated by wound-healing and matrigel invasion chamber assays. Adhesion to type IV collagen and laminin was evaluated by MTT assay. Expression and protease activity of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were analyzed by Western blotting and gelatin zymography, respectively. Results: PPSS exerted growth inhibitory effects on A549 cells, and effectively inhibited A549 cell adhesion, migration and invasion in a concentration-dependent manner. Western blotting and gelatin zymography analysis revealed that PPSS inhibited the expression and secretion of MMP-2 and MMP-9 in A549 cells. Conclusions: PPSS has the potential to suppress the migration, adhesion and invasion of A549 cells. PPSS could be a potential candidate for interventions against lung cancer metastases.

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Section: Discussionsupporting

confidence: 59%

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9

He¹,

Zheng²,

Sun³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…Matrix metalloproteinases (MMPs), which are molecules that are implicated in metastasis by various malignant tumors, have been investigated for their role in contributing to the ability of EGISTs to metastasize. A recent study demonstrated that increased MMP-2 and MMP-9 expression was associated with increased risk of metastasis and aggressive behavior in E-GISTs (Wang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal Stromal Tumors: A Clinicopathologic and Risk Stratification Study of 255 Cases from Pakistan and Review of Literature

Din¹,

Ahmad²,

Arshad³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Purpose: To describe the clinicopathological features of gastrointestinal stromal tumors (GIST) diagnosed in our section and to perform risk stratification of our cases by assigning them to specific risk categories and groups for disease progression based on proposals by Fletcher et al and Miettinen and Lasota. Materials and Results: We retrieved 255 cases of GIST diagnosed between 2003 and 2014. Over 59% were male. The age range was 16 to 83 years with a mean of 51 years. Over 70% occurred between 40 and 70 years of age. Average diameter of tumors was 10 cms. The stomach was the most common site accounting for about 40%. EGISTs constituted about 16%. On histologic examination, spindle cell morphology was seen in almost of 85% cases. CD117 was the most useful immunohistochemical antibody, positive in 98%. Risk stratification was possible for 220 cases. Based on Fletcher's consensus proposal, 62.3 gastric, 81.8% duodenal, 68% small intestinal, 72% colorectal and 89% EGISTs were assigned to the high risk category; while based on Miettinen and Lasota's algorithm, about 48% gastric, 100% duodenal, 76% small intestinal, 100% colorectal and 100% EGISTs in our study were associated with high risk for disease progression, tumor metastasis and tumor related death. Follow up was available in 95 patients; 26 were dead and 69 alive at follow up. Most of the patients who died had high risk disease and on average death occurred just a few months to a maximum of one to two years after initial surgical resection. Conclusions: Epidemiological and morphologic findings in our study were similar to international published data. The majority of cases in our study belonged to the high risk category.

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“…The role of MMPs in tumor metastasis mainly includes: 1) Destructing local tissue structure and promoting tumor growth; 2) destructing the basement membrane barrier, which facilitates tumor metastasis; 3) promoting tumor neovascularization by improving the ECM. The degradation of ECM mainly depends on proteolytic enzymes; MMPs are an extremely important type of proteolytic enzyme (42,43). MMPs are Zn 2+ -and Ca 2+ -dependent proteases that may be classified by four types according to specific substrates: 1) Collagenase (MMP-1, -8 and -13); 2) gelatinase (MMP-2 and -9); 3) stromelysin (MMP-3, -7, -10 and -11); 4) Membrane type metal protein enzymes (MMP-14, -15, -16 and -17) (44,45).…”

Section: Discussionmentioning

confidence: 99%

The restraining effect of baicalein and U0126 on human cervical cancer cell line HeLa

Zhang

Wang

et al. 2017

Molecular Medicine Reports

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To explore the restraining effect of baicalein and the mitogen-activation protein kinase kinase inhibitor, U0126, on human cervical cell line HeLa proliferation, apoptosis and migration. HeLa cells were treated by different concentrations of baicalein or U0126. A Cell Counting Kit (CCK)‑8 assay was applied to examine cell viability. Flow cytometry was used to determine cell cycle and apoptosis. A wound healing assay was performed to detect cell migration. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay was adopted to test cell apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis was used to detect apoptosis gene and protein expression. CCK‑8 assay demonstrated that baicalein and U0126 suppressed HeLa cell viability by dose dependence. TUNEL, Annexin V‑fluorescein isothiocyanate/propidium iodide, and ratio of Bcl‑2‑associated X protein and B cell lymphoma 2 indicated that baicalein and U0126 induced HeLa cell apoptosis. Flow cytometry revealed that baicalein blocked the cell cycle of HeLa in G0/G1 phase. A wound healing assay demonstrated that baicalein significantly inhibited HeLa cell migration compared with control. Baicalein and U0126 markedly downregulated extracellular signal‑regulated kinase 1/2, matrix metalloproteinase (MMP) 2 and MMP9 levels both in mRNA and protein. In the present study, the authors demonstrated that baicalein and U0126 may be used in cervical cancer treatment by inhibiting cell migration and inducing cell apoptosis.

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Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

Cited by 11 publications

References 27 publications

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9

Gastrointestinal Stromal Tumors: A Clinicopathologic and Risk Stratification Study of 255 Cases from Pakistan and Review of Literature

The restraining effect of baicalein and U0126 on human cervical cancer cell line HeLa

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