Increased expression of microRNA-503 and reduced expression of kangai-1 in B-cell non-Hodgkin's lymphoma

Wu, Jingjing; Li, Aimin; Zhang, Pengyu; Sun, Zhenchang; Nan, Feifei; Geng, Lina

doi:10.3892/etm.2016.2971

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…Until now, the function studies of miR‐503 mainly focus on the tumor biology behaviors such as its occurrence, development, invasion, and metastasis (Zhou et al, ; Li et al, ; Long et al, ). Meanwhile, recent study also revealed that accompanied with reduced expression of KAI1, miR‐503 expression was significantly increased in B‐cell non‐Hodgkin's lymphoma (B‐NHL) patients, indicating that it may be applied as a novel therapeutic and diagnostic marker (Wu et al, ). MiR‐503 also functions as an important epigenetic regulator to promote cardiac fibrosis via miR‐503‐Apelin‐ 13‐TGF‐β‐CTGF‐collagen production pathway (Zhou et al, ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

Liu

et al. 2016

Cell Biology International

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Cyclical stretch-induced bone formation during orthodontic treatment is a complex biological process modulated by various factors including miRNAs and their targeted-gene network. However, the miRNA expression profile and their roles in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) exposed to mechanical stretch remains unclear. Here, we use the miRNA microarray assay to screen for mechano-sensitive miRNAs during stretch-induced osteogenic differentiation of BMSCs and identified that nine miRNAs were differentially expressed between stretched and control BMSCs. Furthermore, miR-503-5p, which was markedly downregulated in both microarray assay and qRT-PCR assay were selected for further functional verification. We found that overexpression of miR-503-5p in BMSCs attenuated stretch-induced osteogenic differentiation while the effect was reversed by miR-503-5p inhibition treatment. In vivo studies, overexpression of miR-503-5p with specific agomir decreased Runx2, ALP mRNA, and protein expression, decreased osteoblast numbers and osteoblastic bone formation in the OTM tension sides. In conclusion, our study revealed that miR-503-5p functions as the mechano-sensitive miRNA and inhibits BMSCs osteogenic differentiation subjected to mechanical stretch and bone formation in OTM tension sides.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

Liu

et al. 2016

Cell Biology International

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The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway

Shi

et al. 2018

Lasers Med Sci

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Photobiomodulation therapy (PBMT) has been demonstrated as regulating osteoblast proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in osteoblast, but the role of miRNAs in the PBMT-based promotion of osteoblast proliferation remains unclear. This study aimed to investigate the effects of PBMT treatment (3.75 J/cm) on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via the miR-503/Wnt3a pathway; meanwhile, detect the expressions of miR-503 and Wnt3a after PBMT treatment and the role of miR-503 in regulating Wnt signaling molecules Wnt3a, β-catenin, Runx2, apoptotic proteins caspase-3, and Bcl-2 in vitro. The PBMT parameters were as follows: 808 nm continuous wavelength, 0.401 W output power, 0.042 W/cm power density, 9.6 cm spot size, 36 J energy, 3.75 J/cm energy density, 90 s irradiation for three times per 12 h, 14.5 cm distance of the laser source and the angle of divergence of the laser beam was 7°. In our present study, the target relationship was predicted and verified by bioinformatics analysis and luciferase reporter assays. Gene mRNA and protein expressions were examined by qPCR and western blot analysis. The MTT method was used to evaluate the effect of miR-503 on MC3T3-E1 cells proliferation. And cell apoptosis was examined by flow cytometry. The results showed that PBMT treatment reduced the expression of miR-503 and increased the level of Wnt3a (p < 0.01). Bioinformatics analysis and luciferase reporter assays revealed that Wnt3a was a target of miR-503, and Wnt3a was regulated by miR-503. Furthermore, miR-503 was found to functionally inhibit proliferation and promote apoptosis (p < 0.01). And during this process, Wnt3a, β-catenin, Runx2, and Bcl-2 expressions were significantly inhibited (p < 0.01); however, caspase-3 level was upregulated (p < 0.01). These results suggest that miR-503 plays a role in osteoblast proliferation and apoptosis in response to PBMT, which is potentially amenable to therapeutic manipulation for clinical application.

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The microRNA-424/503 cluster: A master regulator of tumorigenesis and tumor progression with paradoxical roles in cancer

Wang

Yang

et al. 2020

Cancer Letters

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Increased expression of microRNA-503 and reduced expression of kangai-1 in B-cell non-Hodgkin's lymphoma

Cited by 3 publications

References 28 publications

MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

MicroRNA‐503‐5p inhibits stretch‐induced osteogenic differentiation and bone formation

The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway

The microRNA-424/503 cluster: A master regulator of tumorigenesis and tumor progression with paradoxical roles in cancer

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