Increased expression of histamine H1 receptor mRNA in allergic rhinitis

IRIYOSHI, N.; TAKEUCHI, K.; YUTA, A.; UKAI, K.

doi:10.1046/j.1365-2222.1996.d01-323.x

Cited by 27 publications

(26 citation statements)

References 17 publications

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“…Few studies have evaluated HRH1 expression among those with asthma and/or allergic disease (37, 38) . We have previously shown that HRH1 mRNA expression was significantly up-regulated in adults (p=0.04) with asthma compared to those without asthma (10) .…”

Section: Discussionmentioning

confidence: 99%

Genetic variation within the histamine pathway among patients with asthma – a pilot study

et al. 2014

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Objective Histamine is an important mediator in the pathophysiology of asthma. We have previously reported that HRH1 is differentially expressed among those with asthma compared to those without asthma. Single histamine related genes have also been associated with asthma. We aimed to evaluate known single nucleotide polymorphisms (SNPs) in genes along the histamine biotransformation and response pathway and determine their association with asthma and HRH1 mRNA expression. Methods We enrolled children and adults (n=93) with/without asthma who met inclusion/exclusion criteria. Genotyping was performed for 9 known SNPs in the HDC, HRH1, HRH4, HNMT, and ABP1 genes. HRH1 mRNA expression was determined on RNA from buccal tissue. General linear model, Fisher's exact test, and Chi-square test were used to determine differences in allele, genotype, and haplotype frequency between subjects with and without asthma and differential HRH1 mRNA expression relative to genotype. Statistical significance was determined by p<0.05. Results No difference was observed in genotype/allele frequency for the 9 SNPs between subjects with and without asthma. The HNMT-1639C/ −464C/ 314C/ 3’UTRA haplotype was more frequently observed in those without asthma than those with asthma (p=0.03). We also observed genetic differences relative to race and gender. HNMT 314 genotype CT was more frequent in males with asthma compared to those without asthma (p=0.04). Conclusions Histamine pathway haplotype was associated with a diagnosis of asthma in our cohort but allele and genotype were not. Subgroup evaluations may also be important. Further studies are needed to determine the potential biological/clinical significance of our findings.

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Section: Discussionmentioning

confidence: 99%

Genetic variation within the histamine pathway among patients with asthma – a pilot study

et al. 2014

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“…H1R mRNA expression was increased in epithelial, endothelial, and neural cells of the nasal mucosa in patients with occupational rhinitis23. The up-regulation of H1R gene was also observed in patients with allergic rhinitis45. Terada et al reported that H1R mRNA expression increased in human nasal epithelial cells after stimulation with diesel exhaust particles known to cause chronic airway diseases27.…”

Section: Discussionmentioning

confidence: 99%

“…H1R mRNA expression has been reported to increase in epithelial, endothelial, and neural cells of the nasal mucosa in patients with occupational rhinitis23. The up-regulation of H1R gene expression was also observed in patients with allergic rhinitis45, and H1R binding in the nasal mucosa was reported to increase during the development of nasal allergies6.…”

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confidence: 99%

PMA-induced dissociation of Ku86 from the promoter causes transcriptional up-regulation of histamine H1 receptor

Mizuguchi

Miyagi

Terao

et al. 2012

Sci Rep

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Histamine H1 receptor (H1R) gene is up-regulated in patients with allergic rhinitis, and its expression level strongly correlates with the severity of symptoms. However, the mechanism underlying this remains unknown. Here we report the mechanism of H1R gene up-regulation. The luciferase assay revealed the existence of two promoter regions, A and B1. Two AP-1 and one Ets-1 bound to region A, while Ku86, Ku70, and PARP-1 bound to region B1. Ku86 was responsible for DNA binding and poly(ADP-ribosyl)ated in response to phorbol-12-myristate-13-acetate stimulation, inducing its dissociation from region B1 that is crucial for promoter activity. Knockdown of Ku86 gene enhanced up-regulation of H1R gene expression. Experiments using inhibitors for MEK and PARP-1 indicate that regions A and B1 are downstream regulatory elements of the PKCδ/ERK/PARP-1 signaling pathway. Data suggest a novel mechanism for the up-regulation of H1R gene expression.

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“…By using RT-PCR, H1 receptor mRNA levels have been found to be increased in nasal mucosa of perennial allergic rhinitis [10, 11], but not in seasonal allergic rhinitis [12]. On the other hand, little work has been done to evaluate the expression of nasal H1 receptor at protein level.…”

Section: Discussionmentioning

confidence: 99%

Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis

Shirasaki

Kanaizumi

Seki

et al. 2012

Mediators of Inflammation

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In the present study, we have investigated the expression of histamine H1 receptor in human turbinates by RT-PCR, western blotting, and immunohistochemistry. Human turbinates were obtained by turbinectomy from 12 patients with nasal obstruction refractory to medical therapy. RT-PCR analysis of total RNA extracted from human nasal turbinate, primary cultured human nasal epithelial cells, and nasal vascular endothelial cells demonstrated the expression of histamine H1 receptor mRNA. About 56 kDa band was detected in human turbinates by western blot analysis using anti-H1 receptor antibody. The expression level of H1 receptor protein was marked in patients with nasal allergy than in patients with nonallergic rhinitis. The immunohistochemical study revealed that epithelial cells and vascular endothelial cells showed intense immunoreactivity for histamine H1 receptor. In addition, the blood vessels in superficial area expressed higher level of H1 receptor immunoreactivity than that in deeper area in the nasal mucosa. These results may have an important clinical implication for understanding the role of histamine H1 receptor on upper airway diseases such as allergic rhinitis and nonallergic rhinitis.

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Increased expression of histamine H1 receptor mRNA in allergic rhinitis

Abstract: These data suggest that expression of H1R mRNA is increased in the nasal mucosa of the patients with allergic rhinitis.

Cited by 27 publications

References 17 publications

Genetic variation within the histamine pathway among patients with asthma – a pilot study

Genetic variation within the histamine pathway among patients with asthma – a pilot study

PMA-induced dissociation of Ku86 from the promoter causes transcriptional up-regulation of histamine H1 receptor

Localization and Upregulation of the Nasal Histamine H1 Receptor in Perennial Allergic Rhinitis

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