Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components

Yu, Kyung Ok; Jung, Ju Yeon; Ramzi, Ahmad Bazli; Choe, Se Hoon; Kim, Seung Wook; Park, Chulhwan; Han, Sung Ok

doi:10.1016/j.enzmictec.2012.07.003

Cited by 18 publications

(13 citation statements)

References 20 publications

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“…b, 4b, and 5a). These results suggested that the regulation of cellular membrane components might be correlated with the enhanced ethanol tolerance of the ethanol‐tolerant strains .…”

Section: Discussionmentioning

confidence: 87%

“…Ergosterol is the most abundant sterol in the S. cerevisiae cell plasma membrane and plays important role in stabilizing the yeast plasma membrane . Ergosterol content directly correlates with ethanol tolerance of S. cerevisiae . The ATPase activity of the plasma membrane and ethanol tolerance were proven to be correlated at the 96.64% level with oleic acid and ergosterol in yeast plasma membrane .…”

Section: Discussionmentioning

confidence: 98%

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Changes and roles of membrane compositions in the adaptation of Saccharomyces cerevisiae to ethanol

Wang

Zhang

Liu

et al. 2015

J. Basic Microbiol.

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Bioethanol fermentation by Saccharomyces cerevisiae is often stressed by the accumulation of ethanol. Cell membrane is the first assaulting target of ethanol. Ethanol-adapted S. cerevisiae strains provide opportunity to shed light on membrane functions in the ethanol tolerance. This study aimed at clarifying the roles of cell membrane in the ethanol tolerance of S. cerevisiae through comparing membrane components between S. cerevisiae parental strain and ethanol-adapted strains. A directed evolutionary engineering was performed to obtain the ethanol-adapted S. cerevisiae strains. The parental, ethanol-adapted M5 and M10 strains were selected to be compared the percentage of viable cells after exposing to ethanol stress and cell membrane compositions (i.e., ergosterol, trehalose, and fatty acids). Compared with the parental strain, M5 or M10 strain had higher survival rate in the presence of 10% v/v ethanol. Compared with that in the parental strain, contents of trehalose, ergosterol, and fatty acids increased about 15.7, 12.1, and 29.3%, respectively, in M5 strain, and about 47.5, 107.8, and 61.5%, respectively, in M10 strain. Moreover, expression differences of genes involved in fatty acids metabolisms among the parental, M5 and M10 strains were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), and results demonstrated that M5 or M10 strain had higher expression of ACC1 and OLE1 than the parental strain. These results indicated that although being exposed to step-wise increased ethanol, S. cerevisiae cells might remodel membrane components or structure to adapt to the ethanol stress.

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“…b, 4b, and 5a). These results suggested that the regulation of cellular membrane components might be correlated with the enhanced ethanol tolerance of the ethanol‐tolerant strains .…”

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 98%

Changes and roles of membrane compositions in the adaptation of Saccharomyces cerevisiae to ethanol

Wang

Zhang

Liu

et al. 2015

J. Basic Microbiol.

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“…Lipid bilayers in industrial strains of S. cerevisiae contain up to five-fold more ergosterol than laboratory strains; the former can produce 19.2% (v/v) ethanol compared to only 14.2% for the latter [53]. Although it is possible that additional factors contribute to this difference (Table 2), other studies also provide evidence for key roles of ergosterol in tolerance to osmotic and ethanolinduced stresses [54].…”

Section: Microbial Responses and Adaptations To Chaotropicity-inducedmentioning

confidence: 95%

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Cray

Stevenson

Ball

et al. 2015

Current Opinion in Biotechnology

165

174

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“…Different microorganisms, including yeast [2], [3], Zymomonas mobilis [4], [5] and E. coli [6], [7] have been engineered for selective production of ethanol. The highest reported ethanol yield attained through E. coli xylose fermentation is around 60 g/l [8].…”

Section: Introductionmentioning

confidence: 99%

Improving Ethanol Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP)

Chong

Huang

Yeow³

et al. 2013

PLoS ONE

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A major challenge in bioethanol fermentation is the low tolerance of the microbial host towards the end product bioethanol. Here we report to improve the ethanol tolerance of E. coli from the transcriptional level by engineering its global transcription factor cAMP receptor protein (CRP), which is known to regulate over 400 genes in E. coli. Three ethanol tolerant CRP mutants (E1– E3) were identified from error-prone PCR libraries. The best ethanol-tolerant strain E2 (M59T) had the growth rate of 0.08 h−1 in 62 g/L ethanol, higher than that of the control at 0.06 h−1. The M59T mutation was then integrated into the genome to create variant iE2. When exposed to 150 g/l ethanol, the survival of iE2 after 15 min was about 12%, while that of BW25113 was <0.01%. Quantitative real-time reverse transcription PCR analysis (RT-PCR) on 444 CRP-regulated genes using OpenArray® technology revealed that 203 genes were differentially expressed in iE2 in the absence of ethanol, whereas 92 displayed differential expression when facing ethanol stress. These genes belong to various functional groups, including central intermediary metabolism (aceE, acnA, sdhD, sucA), iron ion transport (entH, entD, fecA, fecB), and general stress response (osmY, rpoS). Six up-regulated and twelve down-regulated common genes were found in both iE2 and E2 under ethanol stress, whereas over one hundred common genes showed differential expression in the absence of ethanol. Based on the RT-PCR results, entA, marA or bhsA was knocked out in iE2 and the resulting strains became more sensitive towards ethanol.

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Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components

Cited by 18 publications

References 20 publications

Changes and roles of membrane compositions in the adaptation of Saccharomyces cerevisiae to ethanol

Changes and roles of membrane compositions in the adaptation of Saccharomyces cerevisiae to ethanol

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Improving Ethanol Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP)

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