Increased electrocatalyzed performance through hairpin oligonucleotide aptamer-functionalized gold nanorods labels and graphene-streptavidin nanomatrix: Highly selective and sensitive electrochemical biosensor of carcinoembryonic antigen

Wen, Wei; Huang, Jingyi; Bao, Ting; Zhou, Jun; Hong-xing, Xia; Zhang, Xun; Wang, Shengfu; Zhao, Yuan‐Di

doi:10.1016/j.bios.2016.04.039

Cited by 67 publications

(19 citation statements)

References 36 publications

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“…Aptamer-based assays challenged to clinical serum samples (from healthy individuals and cancer patients) showed satisfactory agreement with the immunomethod routinely used in hospitals (Wen et al, 2016;Ma et al, 2018a;Liu et al, 2019;Wang et al, 2019b). Nevertheless, their complexity in steps and reagents (Wen et al, 2016;Ma et al, 2018a;Liu et al, 2019) could discourage their implementation in clinical practice. Of greater simplicity are signal-off tests (Wang et al, 2019b), although they require very strict selectivity controls.…”

Section: Dna-based Red Diagnosticsmentioning

confidence: 93%

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Miranda‐Castro

Palchetti

de‐los‐Santos‐Álvarez

2020

Front. Chem.

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Latest technological advancement has tremendously expanded the knowledge on the composition of body fluids and the cancer-associated changes, which has fueled the replacement of invasive biopsies with liquid biopsies by using appropriate specific receptors. DNA emerges as a versatile analytical reagent in electrochemical devices for hybridization-based or aptamer-based recognition of all kind of biomarkers. In this mini review, we briefly introduce the current affordable targets (tumor-derived nucleic acids, circulating tumor cells and exosomes) in body fluids, and then we provide an overview of selected electrochemical methods already applied in clinical samples by dividing them into three large categories according to sample type: red (blood), yellow (urine), and white (saliva and sweat) diagnostics. This review focuses on the hurdles of the complex matrices rather than a comprehensive and detailed revision of the format schemes of DNA-based electrochemical sensing. This diverse perspective compiles some challenges that are often forgotten and critically underlines real sample analysis or clinical validation assays. Finally, the needs and trends to reach the market are briefly outlined.

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Section: Dna-based Red Diagnosticsmentioning

confidence: 93%

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Miranda‐Castro

Palchetti

de‐los‐Santos‐Álvarez

2020

Front. Chem.

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“…Amino-groups of capture antibodies and amino-functionalized carbon NP composites, e.g., amino-groups of chitosan, are usually linked using glutaraldehyde (GLA coupling, see e.g., [ 24 ]). Other less frequently used methods to immobilize biomarker capture antibodies utilizes biospecific lectin-sugarprotein interaction [ 25 ], biotin-streptavidin interaction [ 26 ], and bonding between amino groups and Pt [ 22 ].…”

Section: Construction Of Biosensors For Biomarkers Detectionmentioning

confidence: 99%

“…The fabrication and detection process of the sensor is shown in Figure 4 . Wen et al [ 26 ] constructed a sandwich-type CEA biosensor based on triplex signal amplification strategy and on oligonucleotide aptamer capture probes. The sensor platform was prepared by casting a cetyltrimetylammonium bromide (CTAB)-GR suspension on GCE, followed by a chitosan (CS)-streptavidin (SA) solution.…”

Section: Detection Of Cancer and Disease Biomarkersmentioning

confidence: 99%

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

Pasinszki

Krebsz

Tung

et al. 2017

Sensors

140

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The early diagnosis of diseases, e.g., Parkinson’s and Alzheimer’s disease, diabetes, and various types of cancer, and monitoring the response of patients to the therapy plays a critical role in clinical treatment; therefore, there is an intensive research for the determination of many clinical analytes. In order to achieve point-of-care sensing in clinical practice, sensitive, selective, cost-effective, simple, reliable, and rapid analytical methods are required. Biosensors have become essential tools in biomarker sensing, in which electrode material and architecture play critical roles in achieving sensitive and stable detection. Carbon nanomaterials in the form of particle/dots, tube/wires, and sheets have recently become indispensable elements of biosensor platforms due to their excellent mechanical, electronic, and optical properties. This review summarizes developments in this lucrative field by presenting major biosensor types and variability of sensor platforms in biomedical applications.

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“…Modification of the electrode surface by novel nanomaterials is an effective step to improve the performance of electrochemical aptasensors 32 . Due to the advantages of gold nanostructures including high surface area and electron transfer rate, and unique chemical characteristics, they are promising nanomaterials for immobilization of aptamers 33 , 34 . In addition, gold nanostructures are being used to improve the selectivity and sensitivity of detection 28 , 33 – 37 .…”

Section: Introductionmentioning

confidence: 99%

“…Due to the advantages of gold nanostructures including high surface area and electron transfer rate, and unique chemical characteristics, they are promising nanomaterials for immobilization of aptamers 33 , 34 . In addition, gold nanostructures are being used to improve the selectivity and sensitivity of detection 28 , 33 – 37 . A combination of aptamers with nanomaterials to fabricate electrochemical PSA biosensors provide sensitive and specific methods of PSA determination 5 , 34 , 38 .…”

Section: Introductionmentioning

confidence: 99%

A signal-on built in-marker electrochemical aptasensor for human prostate-specific antigen based on a hairbrush-like gold nanostructure

Sattarahmady

Rahi

Heli

2017

Sci Rep

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A green electrodeposition method was firstly employed for the synthesis of round hairbrush-like gold nanostructure in the presence of cadaverine as a size and shape directing additive. The nanostructure which comprised of arrays of nanospindles was then applied as a transducer to fabricate a signal-on built in-marker electrochemical aptasensor for the detection of human prostate-specific antigen (PSA). The aptasensor detected PSA with a linear concentration range of 0.125 to 128 ng mL−1 and a limit of detection of 50 pg mL−1. The aptasensor was then successfully applied to detect PSA in the blood serum samples of healthy and patient persons.

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Increased electrocatalyzed performance through hairpin oligonucleotide aptamer-functionalized gold nanorods labels and graphene-streptavidin nanomatrix: Highly selective and sensitive electrochemical biosensor of carcinoembryonic antigen

Cited by 67 publications

References 36 publications

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

The Translational Potential of Electrochemical DNA-Based Liquid Biopsy

Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis

A signal-on built in-marker electrochemical aptasensor for human prostate-specific antigen based on a hairbrush-like gold nanostructure

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