Increased Efficacies in Magnetofection and Gene Delivery to Hepatocellular Carcinoma Cells with Ternary Organic–Inorganic Hybrid Nanocomposites

Leung, Ken Cham‐Fai; Chak, Chun Pong; Lee, Siu Fung; Lai, Josie M.Y.; Zhu, Xiao; Wáng, Yì Xiáng J.; Sham, Kathy W.Y.; Wong, Chi Hin; Chen, Cheng

doi:10.1002/asia.201300202

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Thus, the diffusion barrier to nucleic acid delivery may be overcome. Via magnetofection the efficiency of gene delivery can be enhanced up to several hundred fold. − …”

Section: Introductionmentioning

confidence: 99%

Enhanced Gene Expression Promoted by Hybrid Magnetic/Cationic Block Copolymer Micelles

Haladjova

Rangelov²,

Tsvetanov³

et al. 2014

Langmuir

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We report on novel gene delivery vector systems based on hybrid polymer-magnetic micelles. The hybrid micelles were prepared by codissolution of hydrophobically surface modified iron oxide and amphiphilic polystyrene-b-poly(quaternized 2-vinylpyridine) block copolymer (PS-b-P2QVP) in organic solvent. After extensive dialysis against water, micelles with positively charged hydrophilic corona of PQVP and hydrophobic PS core were prepared, in which magnetic nanoparticles were randomly distributed. The hybrid micelles were used to form complexes with linear (salmon sperm, 2000 bp, corresponding to M(w) of 1.32 × 10(6) Da) and plasmid (pEGFP-N1, 4730 bp, corresponding to M(w) of 3.12 × 10(6) Da) DNA. The resulting magnetopolyplexes of phosphate:amine (P/N) ratios in the 0.05-20 range were characterized by light scattering, ζ-potential measurements, and transmission electron microscopy as well as cytotoxicity and gel retardation assays. The investigated systems displayed a narrow size distribution, particle dimensions below 360 nm, whereas their ζ-potential values varied from positive to negative depending of the P/N ratio. The resulting vector nanosystems exhibited low toxicity. They were able to introduce pEGFP-N1 molecules into the cells. The application of a magnetic field markedly boosted the transgene expression efficiency of the magnetopolyplexes, which was even superior to those of commercial transfectants such as Lipofectamine and dendritic polyethylenimine.

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“…Thus, the diffusion barrier to nucleic acid delivery may be overcome. Via magnetofection the efficiency of gene delivery can be enhanced up to several hundred fold. − …”

Section: Introductionmentioning

confidence: 99%

Enhanced Gene Expression Promoted by Hybrid Magnetic/Cationic Block Copolymer Micelles

Haladjova

Rangelov²,

Tsvetanov³

et al. 2014

Langmuir

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“…Such agents are currently under clinical evaluation. [1] Key clinical trials for transcatheter arterial therapy for liver cancer [2][3][4][5] and the recent patents relevant to cancer chemoembolization are being developed. Herein, we describe the use of a hybrid nanoshell/drug/ polymer composite as a chemoembolization agent for transarterial delivery in liver tumors.…”

mentioning

confidence: 99%

In Vivo Chemoembolization and Magnetic Resonance Imaging of Liver Tumors by Using Iron Oxide Nanoshell/Doxorubicin/Poly(vinyl alcohol) Hybrid Composites

et al. 2014

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A hybrid composite made up of superparamagnetic iron oxide nanoshells encapsulating the anticancer drug doxorubicin and bound together by poly(vinyl alcohol) was developed. Transcatheter arterial delivery in an in vivo liver tumor model led to embolization of the liver tumor blood vessels. Embolization was followed by disassembly of the composite. The nanoshells were then able to pass through the leaky tumor vasculature into the tumor tissue, thereby leading to slow and sustained release of the drug. As well as being relatively noncytotoxic, the composite was responsive to magnetic resonance imaging, thus making it a potentially useful theranostic agent.

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“…Electroporation has been used for delivering TRAIL/Apo2L gene to induce apoptosis [145]; the IL-12 gene to induce immune responses to HCC [146,147]; more recently, to deliver mRNA into T-cells to develop specific T-cells for HCC immunotherapy [148]; and GPC-3 CAR-T-cells [149]. Magnetofection has also been used to deliver genes into HCC cell lines combined with ternary organic-inorganic hybrid nanocomposites containing deferoxamine-coated iron oxide nanoparticles, plasmid DNA, and branched polyethyleneimine [150]. Hydrodynamic gene delivery has been used for functional analysis of therapeutic genes and regulatory elements in rodents since its establishment in 1999 [151,152].…”

Section: Non-viral Gene Delivery Using Physical Methodsmentioning

confidence: 99%

Gene Therapy for Liver Cancers: Current Status from Basic to Clinics

Kamimura

Yokoo

Abé

et al. 2019

Cancers

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The liver is a key organ for metabolism, protein synthesis, detoxification, and endocrine function, and among liver diseases, including hepatitis, cirrhosis, malignant tumors, and congenital disease, liver cancer is one of the leading causes of cancer-related deaths worldwide. Conventional therapeutic options such as embolization and chemotherapy are not effective against advanced-stage liver cancer; therefore, continuous efforts focus on the development of novel therapeutic options, including molecular targeted agents and gene therapy. In this review, we will summarize the progress toward the development of gene therapies for liver cancer, with an emphasis on recent clinical trials and preclinical studies.

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Increased Efficacies in Magnetofection and Gene Delivery to Hepatocellular Carcinoma Cells with Ternary Organic–Inorganic Hybrid Nanocomposites

Cited by 11 publications

References 24 publications

Enhanced Gene Expression Promoted by Hybrid Magnetic/Cationic Block Copolymer Micelles

Enhanced Gene Expression Promoted by Hybrid Magnetic/Cationic Block Copolymer Micelles

In Vivo Chemoembolization and Magnetic Resonance Imaging of Liver Tumors by Using Iron Oxide Nanoshell/Doxorubicin/Poly(vinyl alcohol) Hybrid Composites

Gene Therapy for Liver Cancers: Current Status from Basic to Clinics

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