Increased Durability of Resin-Dentin Bonds Following Cross-Linking Treatment

Abstract

Objectives This study evaluated the long-term effect of carbodiimide treatments of acid-etched dentin on resin-dentin bond strength of a simplified etch-and-rinse adhesive system. Methods Forty-eight sound third molars were divided into three groups (n=16) according to the dentin treatment: G1: Deionized water; G2: 0.5 mol/L 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) applied for 30 seconds; and G3: 0.5 mol/L EDC applied for 60 seconds. Flat dentin surfaces were produced, etched with 37% phosphoric acid for 15 seconds and then treated with deionized water for 60s or with 0.5 mol/L EDC for 30 or 60 seconds prior to the application of Single Bond 2. Crowns were restored with resin composite and beam specimens were prepared for microtensile testing. The beams from each group were tested 24 hours or 6 or 12 months after the adhesive procedures. One slab from each tooth was prepared and analyzed for nanolaekage. Bond strength (MPa) data were submitted to analysis of variance and Tukey test (α=0.05). Results The treatment of dentin with 0.5 mol/L EDC for 30 seconds (24.1±6.2 MPa) and 60 seconds (25.5±5.1 MPa) did not negatively affect the immediate bond strength of Single Bond 2 when compared to the control group (24.6±7.3 MPa). Additionally, EDC prevented resin-dentin bond degradation after 12 months in artificial saliva for both periods of treatment. An increased accumulation of silver ions was seen for the control group over time, while a much lower amount of silver grains was observed for the EDC-treated groups. Conclusions 0.5 mol/L EDC was able to prevent resin-dentin bond degradation after 12 months, especially when applied for 60 seconds.

Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells

“…33 The crosslinking of dentin collagen using GA and others cross-linkers has been investigated as a promising mechanism to delay the degradation of the hybrid layer 34,35 . For that purpose, GA is applied as a primer on the etched dentin prior to the application of the adhesive system.…”

Experimental primers containing synthetic and natural compounds reduce enzymatic activity at the dentin–adhesive interface under cyclic loading

“…It is well known that the dentin-adhesive interface can be degraded by MMPs [4, 5, 8], therefore the use of inactivators of MMPs seems to be a valid strategy to increase the dentin-resin bond durability [3, 4, 15]. Our first null hypothesis was rejected as there were significant differences among the anti-proteolytic actions of experimental primers on recombinant MMPs and enzymatic activity at the dentin-resin interface.…”

“…The most well investigated synthetic agent to successfully inactivate endogenous proteases at the dentin-adhesive interface is chlorhexidine digluconate (CHX) [5, 7, 11, 12, 13]. Other synthetic inactivators of endogenous proteases with fewer outcomes include tetracycline [4, 7], carbodiimide [14, 15] and galardin [7, 16]. Doxycycline (DOXY) is a tetracycline semi-synthetic analogue, which is considered the most potent and non-selective MMPs inactivating agent among tetracyclines [7, 17].…”

Comparação da resistência de união de diferentes superfícies dentinárias na obtenção dos espécimes para microtração

“…Os testes de microtração têm sido largamente empregados para avaliar a resistência de união dos sistemas adesivos (CARVALHO et al, 1994;SANO et al, 1994;PASHLEY et al, 1999;BURKE et al, 2008;PAZINATTO;ATTA, 2008;VAN LANDUYT et al, 2009;ARMSTRONG et al, 2010;REIS et al, 2010;SCHERRER;CESAR;SWAIN, 2010;VAN MEERBEEK et al, 2010;CABRERA;MACORRA, 2011;EL-SAHN et al, 2011;SAMPAIO et al, 2011;HAN et al, 2012;SODERHOLM;GERALDELI;SHEN, 2012;BAKHSH et al, 2013;BARCELLOS et al, 2013;ISAAC et al, 2013;MOBARAK;EL-DEEB;YOUSRY, 2013;DE LA MACORRA;PEREZ-HIGUERAS, 2014;HAMAMA;YIU;BURROW, 2014;KIM et al, 2014;WAGNER et al, 2014;CHEN et al, 2015;EL-DAMANHOURY;GAINTANTZOPOULOU, 2015;HEINTZE;ROUSSON;MAHN, 2015;MAENOSONO et al, 2015;SCHEFFEL et al, 2015)...…”

“…Portanto, padronizou-se a utilização de dois incrementos horiontais para todos os grupos, não excedendo 2 mm. Azevedo (2011) O objetivo inicial deste trabalho foi comparar a resistência de união entre dois grupos: superfície oclusal planificada, mais comum na literatura (CARVALHO et al, 1994;SANO et al, 1994;TYAS, 1998;SCHREINER et al, 1998;ARMSTRONG et al, 2003;CEBALLOS et al, 2003;HURMUZLU et al, 2007;PROENCA et al, 2007;BRESCHI et al, 2009;CAMPOS et al, 2009;VAN LANDUYT et al, 2009;ARMSTRONG et al, 2010;REIS et al, 2010;MENDEZ et al, 2012;SALVIO et al, 2013;WAGNER et al, 2014;CHEN et al, 2015;MAENOSONO et al, 2015;SCHEFFEL et al, 2015) SENAWONGSE, 2007;SAMPAIO et al, 2011;AZEVEDO, 2011;BAKHSH et al, 2013;ISAAC et al, 2013;EL-DAMANHOURY;GAINTANTZOPOULOU, 2015).…”