Increased concentration of metronidazole and its hydroxy metabolite in colon cancer in women

Sadowska, Anna; Kędra, Bogusław; Cepowicz, Dariusz; Rodziewicz, L; Fiedorowicz, Anna; Prokopiuk, Sławomir; Miltyk, Wojciech; Car, Halina

doi:10.1016/s1734-1140(12)70924-6

Cited by 3 publications

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References 10 publications

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“…15 Our previous study on patients with CRC showed increased accumulation of MTZ in the tumor tissue. 16 Thus, MTZ is used in colon cancer surgery; the main objective of our investigation was to verify the possible effect of MTZ on DLD-1 colon cancer cell line when the dose is repeated.…”

Section: Introductionmentioning

confidence: 99%

Metronidazole Decreases Viability of DLD-1 Colorectal Cancer Cell Line

Sadowska

Krętowski

Szynaka

et al. 2013

Cancer Biotherapy and Radiopharmaceuticals

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The aim of our study was to evaluate the impact of metronidazole (MTZ) on DLD-1 colorectal cancer cell (CRC) line. Toxicity of MTZ was determined by MTT test. Cells were incubated with MTZ used in different concentrations for 24, 48, and 72 hours. The effect of MTZ on DNA synthesis was measured as [3H]-thymidine incorporation. The morphological changes in human DLD-1 cell line were defined by transmission electron microscope OPTON 900. The influence of MTZ on the apoptosis of DLD-1 cell lines was detected by flow cytometry and fluorescence microscopy, while cell concentration, volume, and diameter were displayed by Scepter Cell Counter from Millipore. Our results show that cell viability was diminished in all experimental groups in comparison with the control, and the differences were statistically significant. We did not find any significant differences in [3H]-thymidine incorporation in all experimental groups and times of observation. Cytofluorimetric assays demonstrated a statistically significant increase of apoptotic rate in MTZ concentrations 10 and 50 lg/mL after 24 hours; 0.1, 10, 50, and 250 lg/mL after 48 hours; and in all concentrations after 72 hours compared with control groups. In the ultrastructural studies, necrotic or apoptotic cells were occasionally seen. In conclusion, MTZ affects human CRC cell line viability. The reduction of cell viability was consistent with the apoptotic test.

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Section: Introductionmentioning

confidence: 99%