“…Numerous methods are available in literature to determine MTZ alone (Bolton & Culshaw, ; Cox, Allender, & Yarbrough, ; de Freitas Silva et al, ; Emami, Ghassami, & Hamishehkar, ; Galmier et al, ; Ilomuanya et al, ; Jeffery et al, ; Krook et al, ; Kubodera, Tokumura, & Machida, ; Mustapha, Odunola, Garba, & Obodozie, ; Silva et al, ; Vanol, Sanyal, Shah, & Shrivastav, ; Wibawa, Shaw, & Barrett, ), together with its hydroxy metabolite (Klimowicz, Bielecka‐Grzela, & Tomaszewska, ) and with other drugs like ranitidine (Nascimento, Oliveira, & Macêdo, ) and spiramycin I (Sagan et al, ) in a variety of biological matrices. Analytical techniques such as liquid chromatography with UV (Bolton & Culshaw, ; Cox et al, ; Emami et al, ; Galmier et al, ; Klimowicz et al, ; Krook et al, ; Kubodera et al, ; Mustapha et al, ; Wibawa et al, ), photodiode array (Nascimento et al, ) or mass spectrometry (de Freitas Silva et al, ; Ilomuanya et al, ; Jeffery et al, ; Sagan et al, ; Silva et al, ; Vanol et al, ) detection have been used for the quantification of MTZ in these matrices. The salient features of these methods are assessed in our previous report (Vanol et al, ).…”