Increased circulating endothelial progenitor cells in septic patients: Correlation with survival

Rafat, Neysan; Hanusch, C; Brinkkoetter, Paul T.; Schulte, Jutta; Brade, Joachim; Zijlstra, Jan G.; Woude, Fokko J. van der; Ackern, K. van; Yard, Benito A.; Beck, Grietje

doi:10.1097/01.ccm.0000269034.86817.59

Cited by 114 publications

(154 citation statements)

References 47 publications

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“…The present study demonstrated that MNCs could be separated by a method of Ficoll density gradient centrifugation from rat bone marrow, consistent with previous reports [10,11]. EPCs were isolated primarily in 1997 by Asahara et al [1], and since then has been extensively studied not only in biologic characteristics but functional implication.…”

Section: Discussionsupporting

confidence: 91%

“…EPCs were isolated primarily in 1997 by Asahara et al [1], and since then has been extensively studied not only in biologic characteristics but functional implication. EPCs have been shown to express the endothelial cell surface antigens CD34, KDR and stem cell surface antigens CD133, and uptake AcLDL, so we can isolate or identify by these markers [11-15]. EPCs could be counted as CD34+ CD45 low CD133+ VEGFR2+ cells by flow cytometry [16].…”

Section: Discussionmentioning

confidence: 99%

“…The Ficoll density gradient centrifugation, a previously published method [11,12], was used to isolate mononuclear cells(MNCs) from rat bone marrow. Isolated mononuclear cells were cultured in complete medium 199 without any growth factors (M199, Gibco.…”

Section: Methodsmentioning

confidence: 99%

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The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

et al. 2012

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BackgroundEPCs were isolated primarily in 1997 by Asahara et al. and recent studies indicated that bone-marrow-derived EPCs contributed little to the endothelium of tumor vessels. Tumors of the CNS system demonstrate various features of angiogenesis.MethodsEPCs derived from rat bone marrow were isolated and cultured in M199 medium without any induced factors. EPCs were studied using immunohistochemical staining, Flow cytometry and culture under three-dimensional condition to determine EPCs’ characteristics in vitro. We also established an animal model by injecting EPCs marked with Hoechst 33342 into the back of BALB/c nude mice and performed hematoxylin-eosin (HE) and immunofluorescent staining to study EPCs’ features in vivo. To research effect of EPCs on glioma, animals bearing tumors model with C6 glioma were established. About 27 day after injection, we performed immunohistochemical staining and Immunofluorescence staining.ResultsOur results showed that EPCs derived from rat bone marrow appeared typical morphological characteristics and were positive of CD34, CD133, KDR and CD31 antigens at different time in vitro under the special M199 medium without any induced factors. The percentage of cells that expressed CD133 decreased gradually. In brief, the present study showed that EPCs derived from rat bone marrow differentiated into ECs in medium the without any induced factors and formed tubular structures in three-dimensional circumstances. Animal experiments suggested that EPCs differentiated into ECs and other else non-endothelial cells, and that EPCs contributed M199 of glioma.DiscussionThese findings provides some novel results about biological characteristics of EPCs in vivo and ex vivo, and an update on the effect of EPCs on glioma and which would be helpful for the overall understanding of EPCs and make EPCs to be implied on the clinical therapy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

et al. 2012

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“…These cells are able to differentiate into endothelial cells or VSM cells [42,43,58]. Increased levels of circulating EPCs have been reported in inflammatory respiratory conditions such as pneumonia or sepsis [59]. Additionally, higher levels of circulating EPCs have been found in asthmatic individuals [60], and this is also seen in the lungs of ovalbumin-sensitised mice, with EPC number increasing in response to the potent pro-inflammatory mediator TGF-β and CXCR2 [60,61].…”

Section: Endothelial Progenitor Cellsmentioning

confidence: 99%

Pulmonary vascular changes in asthma and COPD

Harkness

Kanabar

Sharma

et al. 2014

Pulmonary Pharmacology & Therapeutics

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In chronic lung disorders such as in asthma and chronic obstructive pulmonary disease (COPD) there is increased bronchial angiogenesis and remodelling of pulmonary vessels culminating to altered bronchial and pulmonary circulation. The involvement of residential cells such as endothelial cells, smooth muscle cells and pulmonary fibroblasts, all appear to have a crucial role in the progression of vascular inflammation and remodelling. The regulatory abnormalities, growth factors and mediators implicated in the pulmonary vascular changes of asthma and COPD subjects and potential therapeutic targets have been described in this review.

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“…Many of these biomarkers are still considered investigational and only available in highly specialized laboratories. An area of developing interest lies in endothelial microparticles for their diagnostic and prognostic value [46,47], and in EPC mobilization and functionality for their prognostic [9,48] and therapeutic capacity [49 •• ].…”

Section: Plasma Biomarkers Of Endothelial Dysfunctionmentioning

confidence: 99%

Perioperative Implications of Vascular Endothelial Dysfunction: Current Understanding of this Critical Sensor-Effector Organ

Riedel

Rafat

Browne

et al. 2013

Curr Anesthesiol Rep

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The endothelium, a delicate monolayer of cells lining all blood vessels, is a highly responsive sensoreffector organ, which through the secretion of a multitude of mediators, controls vascular tone, interacts with the inflammatory-coagulation cascades, promotes anti-aggregation, regulates immune cellular trafficking, and is crucial to angiogenesis. In brief, the endothelium is a major determinant of vascular homeostasis. It is easily damaged by intrinsic and extrinsic factors, and given its limited capacity for self-repair, is dependent on circulating bone marrow-derived endothelial progenitor cells for regeneration. There is increasing recognition that targeting the endothelium and maximizing inherent physiological function in the perioperative period may improve postoperative outcomes. This review explores the pathogenesis of endothelial dysfunction, the clinical utility of noninvasive methods to assess preoperative endothelial/microvascular (dys)function to improve risk stratification, and potential strategies for perioperative modulation of endothelial function.

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Increased circulating endothelial progenitor cells in septic patients: Correlation with survival

Abstract: Our data suggest that cEPC enumeration in peripheral blood of septic patients might be a valuable marker to assess the clinical outcome in these patients.

Cited by 114 publications

References 47 publications

The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

Pulmonary vascular changes in asthma and COPD

Perioperative Implications of Vascular Endothelial Dysfunction: Current Understanding of this Critical Sensor-Effector Organ

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