The characteristics of bone marrow-derived endothelial progenitor cells and their effect on glioma

Abstract

BackgroundEPCs were isolated primarily in 1997 by Asahara et al. and recent studies indicated that bone-marrow-derived EPCs contributed little to the endothelium of tumor vessels. Tumors of the CNS system demonstrate various features of angiogenesis.MethodsEPCs derived from rat bone marrow were isolated and cultured in M199 medium without any induced factors. EPCs were studied using immunohistochemical staining, Flow cytometry and culture under three-dimensional condition to determine EPCs’ characteristics in vitro. We also established an animal model by injecting EPCs marked with Hoechst 33342 into the back of BALB/c nude mice and performed hematoxylin-eosin (HE) and immunofluorescent staining to study EPCs’ features in vivo. To research effect of EPCs on glioma, animals bearing tumors model with C6 glioma were established. About 27 day after injection, we performed immunohistochemical staining and Immunofluorescence staining.ResultsOur results showed that EPCs derived from rat bone marrow appeared typical morphological characteristics and were positive of CD34, CD133, KDR and CD31 antigens at different time in vitro under the special M199 medium without any induced factors. The percentage of cells that expressed CD133 decreased gradually. In brief, the present study showed that EPCs derived from rat bone marrow differentiated into ECs in medium the without any induced factors and formed tubular structures in three-dimensional circumstances. Animal experiments suggested that EPCs differentiated into ECs and other else non-endothelial cells, and that EPCs contributed M199 of glioma.DiscussionThese findings provides some novel results about biological characteristics of EPCs in vivo and ex vivo, and an update on the effect of EPCs on glioma and which would be helpful for the overall understanding of EPCs and make EPCs to be implied on the clinical therapy.

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase

“…These cells were called late EPCs, and we used late EPCs for further studies. EPCs expressed surface markers, such as CD31 (96.8±0.5%), CD34 (97.2±1.4%), CD133 (92.8±5.3%) and VEGFR-2 (91.5±3.7%) (Figure 1C), and this finding is consistent with previous studies [20, 21]. EPCs could differentiate into adipocytes (Figure 1D) and smooth muscle cells (Figure 1E).…”

Kv1.3 activity perturbs the homeostatic properties of astrocytes in glioma

“…In brain tumors, soluble factors released by glioma-associated myeloid cells (GAMs; mainly macrophages and microglia), promote cell invasion and angiogenesis 18 , 19 . Different K + channels expressed by GAMs modulate phagocytosis, cell migration and proliferation 20 – 22 , with Kv1.3 regulating microglia cell proliferation and neurotoxicity 23 , 24 .…”

KAI1/CD82 Genetically Engineered Endothelial Progenitor Cells Inhibit Metastasis of Human Nasopharyngeal Carcinoma in a Mouse Model

“…Briefly, EPCs were isolated from human umbilical cord blood and cultured in medium containing M199 supplemented with 200 ml/L FBS, 10 μg/L vascular endothelium growth factor (VEGF; eBioscience, USA), 15g/L bovine pituitary extract, and 10 μg/L basic fibroblast growth factor (bFGF; eBioscience, USA) as previously described [ 15 ]. To analyze the function of EPCs, adherent cells were first incubated with 2.4 mg/L of DiI-acetylated low-density lipoprotein (Dil-acLDL) (MolecularProbe, USA), then stained with FITC-Ulex europaeus lectin-1 (FITC-UEA-I) (Sigma, USA).…”