Increased Cell Surface Exposure of Fucose Residues Is a Late Event in Apoptosis

Russell, Lyndal; Waring, Paul; Beaver, Joanne

doi:10.1006/bbrc.1998.9337

Cited by 40 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent data suggests that exposure of fucose residues is a feature of thymocyte apoptosis. 28 However, we found that binding of lotus lectin TGP was reduced on apoptosis neutrophils, suggesting that fucose exposure is not a general feature of apoptotic cell membranes.…”

Section: Neutrophil Surface Carbohydratesmentioning

confidence: 66%

Molecular characterization of the surface of apoptotic neutrophils: Implications for functional downregulation and recognition by phagocytes

et al. 2000

View full text Add to dashboard Cite

We have used a panel of monoclonal antibodies and lectins to examine the profile of surface molecule expression on human neutrophils that have undergone spontaneous apoptosis during in vitro culture. Neutrophil apoptosis was found to be accompanied by down-regulation of the immunoglobulin superfamily members PECAM-1 (CD31), ICAM-3 (CD50), CD66acde, and CD66b and the integrin-associated proteins CD63 and urokinase plasminogen activator receptor (CD87) that may alter the potential for adhesive interactions. Cellular interactions may be further influenced by the reduction of the expression of surface carbohydrate moieties, including sialic acid. Reduced expression of FcgRII (CD32), complement receptor type 1 (CD35) and receptors for pro-inflammatory mediators C5a (CD88) and TNFa (CD120b) associated with apoptosis might limit neutrophil responsiveness to stimuli that trigger degranulation responses. Although many of the receptors we have examined are expressed at reduced levels on apoptotic neutrophils, we found that there was differential loss of certain receptors (e.g. CD16, CD15 and CD120b) and increased expression of aminopeptidase-N (CD13). Together with our previous data showing that expression of certain molecules e.g. LFA-3 (CD58) is not altered during neutrophil apoptosis, these data are suggestive of specific changes in receptor mobilisation and shedding associated with apoptosis. Although reduced expression of CD63 (azurophilic granules) and CR1 (specific granules) indicates that granule mobilisation does not accompany apoptosis, a monoclonal antibody (BOB78), that recognises a 90 kDa antigen localised in intracellular granules, defines a subpopulation of apoptotic neutrophils that exhibit nuclear degradation yet retain intact plasma membranes. BOB78 positive neutrophils were found to bind biotinylated thrombospondin, suggesting that this mAb defines surface molecular changes associated with exposure of thrombospondin binding moieties. Cell Death and Differentiation (2000) 7, 493 ± 503.

show abstract

Section: Neutrophil Surface Carbohydratesmentioning

confidence: 66%

Molecular characterization of the surface of apoptotic neutrophils: Implications for functional downregulation and recognition by phagocytes

et al. 2000

View full text Add to dashboard Cite

show abstract

“…These determinants could function at the level of receptor glycosylation or, alternatively, be analogous to other intracellular glycosylation events [63]. Our results suggest that the proliferative effects of fucosylation are associated with minimal changes in differentiation [35,48,64,65] and apoptosis [66], at least in this poorly differentiated cell line [49]. While we cannot rule out primary effects of tumor suppressor genes or oncogenes in experiments reported here, additional studies will be required to examine these and other potential interactions with fucosylation.…”

Section: Colo-205 Cells Transfected With Fut3/fut6 Antisense Sequencementioning

confidence: 84%

Transfection of ?(1,3)fucosyltransferase antisense sequences impairs the proliferative and tumorigenic ability of human colon carcinoma cells

et al. 2000

View full text Add to dashboard Cite

Sialyl Lewis x and sialyl Lewis a are oncodevelopmental antigens involved in the pathogenesis of colon adenocarcinoma. Biosynthesis of these glycans is controlled by alpha(1,3/1,4)fucosyltransferases. We report the disruption of sialyl Lewis x/a biosynthesis and inhibition of colon carcinoma cell proliferation by stable transfection of antisense sequences directed at the human Lewis alpha(1,3/1,4)fucosyltransferase gene, FUT3, and the plasma alpha(1,3)fucosyltransferase gene, FUT6. COLO-205 cells expressed high levels of sialyl Lewis x/a, alpha(1,3)fucosyltransferase activity, and FUT3/6 transcripts, but COLO

show abstract

“…the enhancement in α (1,2) fucosylation in tumour cells appears to induce a decrease in these sialylated structures, thereby reducing their invasive potential (17). other investigators have suggested that the accessibility of H structures at the cell surface is very important for the motility function and apoptotic cell process (26,29). similarly, the decrease in α(1,2)fucosylation in colorectal tumour cells reduces resistance to anticancer drugs and increases susceptibility to uV treatments (7).…”

Section: Discussionmentioning

confidence: 99%

α(1,2)fucosylation in human colorectal carcinoma

Muinelo-Romay

Gil‐Martín²,

Fernández-Briera³

2010

Oncology Letters

View full text Add to dashboard Cite

Abstract. lewis b and lewis y (le) antigens are known to be elevated in colorectal tumours. Alterations in the catalytic behaviour of gDP-l-fucose:β-D-galactoside α(1,2) fucosyltransferase [α(1,2)Ft, ec: 2.4.1.69], the key enzyme in their synthesis, have been suggested as being responsible for these changes. In particular, an aberrant tumour-specific α(1,2)Ft activity that converts le a and le x to le b and le y determinants, respectively, has been reported in colorectal cancer tissues. to clarify the catalytic function of this enzyme during colorectal tumorigenesis, we analyzed α(1,2)Ft activity levels in healthy and tumour colon specimens using different acceptor substrates and determined the kinetic properties of the enzyme. to complete the study, the aberrant le a /le x α(1,2) fucosylation was determined in healthy and tumour colorectal tissues. A correlation analysis between the activity levels and various standard clinicopathological features, such as tumour stage, was also carried out to elucidate the role of these activities in tumour progression. The results obtained confirm the enhanced α(1,2)fucosylation in colorectal neoplastic tissues and the importance of the aberrant le a /le x α(1,2)Ft activity in this increase. However, taking into account the high levels of le a /le x fucosylation observed in healthy control tissues, we must rule out the idea of a colorectal tumour-specific α(1,2) FT. On the other hand, no significant association was observed between α(1,2)Ft activity levels and the clinicopathological characteristics. overall, our results suggest that α(1,2)Ft activity plays a critical role in the accumulation of le b and le y antigens in human colorectal carcinoma. IntroductionAlterations in glycosylation patterns are one of the characteristics associated with differentiation and malignant transformation (1). the oligosaccharide portions of cellular glycoconjugates, glycolipids and glycoproteins, convey specific immunodeterminants, such as the blood group and related antigens (2). these antigens are known to play an important role in cell recognition, interaction, adhesion and motility. Accordingly, changes in these antigens during carcinogenesis may be crucial in tumour progression and may be involved in extravasation and metastatic phenomena (3).ABo and related determinants are the result of the sequential addition of monosaccharide units to one of the five disaccharide precursors described. type 1 [galβ (1,3)glcnac β1-r] and 2 [gal β(1,4)glcnac β1-r] precursors produce H 1 and H 2 structures, respectively, after the addition of a fucose in α(1,2) linkage to the terminal β-galactosyl residue. H 1 and H 2 determinants can subsequently be converted into le b and le y difucosylated antigens by fucosylation in α(1,4) or α(1,3) linkage, respectively. their positional isomers, the le a and le x antigens, show a similar structure, but the antigens are not fucosylated in α(1,2) (4). Specific alterations of Lewis antigens have been reported in multiple carcinomas and have been correlated with the pr...

show abstract

Increased Cell Surface Exposure of Fucose Residues Is a Late Event in Apoptosis

Cited by 40 publications

References 22 publications

Molecular characterization of the surface of apoptotic neutrophils: Implications for functional downregulation and recognition by phagocytes

Molecular characterization of the surface of apoptotic neutrophils: Implications for functional downregulation and recognition by phagocytes

Transfection of ?(1,3)fucosyltransferase antisense sequences impairs the proliferative and tumorigenic ability of human colon carcinoma cells

α(1,2)fucosylation in human colorectal carcinoma

Contact Info

Product

Resources

About