Increased bile flow rate and altered composition of bile induced by ethanolic leaf extract of Azadirachta indica (neem) in rats

Ofem, Ofem E.; Ikpi, Daniel Ewa; Essien, Nsima Monday

doi:10.4103/2348-0149.123958

Cited by 8 publications

(8 citation statements)

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“…The gastric secretion rate was changed with digestion time with a mean value of 25 µL/min over 180 min digestion through the secretion tubes using a syringe pump, with values based on previous comparisons with in vivo data (Chen et al, 2013). Meanwhile, the mechanical driving instrument of the duodenum device was set to produce 36 contractions per minute (Lentle et al, 2012) and the secreting rates of artificial pancreatic juice and bile juice were set at 30 µL/min (Hotz et al, 1975;Ofem et al, 2013) using the other two syringe pumps. The food materials were digested in the DIVRSD model in separate batches for 10, 20, 30, 40, 60, 90, 120 and 180 min.…”

Section: In Vitro Batch Digestion Of Hydrated Dietsmentioning

confidence: 99%

In vitro digestion of pectin- and mango-enriched diets using a dynamic rat stomach-duodenum model

Bhattarai

Dhital

et al. 2017

Journal of Food Engineering

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In this study we investigated the in vitro digestive behaviors of starch-based diets enriched with soluble fiber (pectin) and fruit powder (mango) varying with dry matter contents using a dynamic rat stomach and duodenum model. The changes in pH with respect to digestion time, gastric emptying, starch and protein hydrolysis as well as rheological and microstructural properties of the gastric and duodenal digesta were investigated. The effects of porcine gastric mucin on digestion were studied as well. The results showed that gastric digestion and emptying rate were decreased with increasing dry matter content of the diets. Both the dynamic and steady shear viscosity of the duodenal digesta was lower than the gastric digesta due to hydrolysis of starch and protein as well as dilution by added digestive juice. With the same dry matter content (20.35%), the rates of gastric emptying as well as starch and protein hydrolysis were higher in the control and mango diets compared to the pectin diet, illustrating the complex relationships among rheology, soluble dietary fiber and digestion. The addition of gastric mucin could delay the rates of gastric digestion and emptying by increasing digesta viscosity. The study emulates the complexity of heterogeneous diets during simulated gastric and duodenal digestion and suggests that the viscosity generated by either addition of mucin on the diet or naturally exerted by e.g. pectin could be the rate limiting factor for both gastric emptying and hydrolysis of starch and proteins.

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Section: In Vitro Batch Digestion Of Hydrated Dietsmentioning

confidence: 99%

In vitro digestion of pectin- and mango-enriched diets using a dynamic rat stomach-duodenum model

Bhattarai

Dhital

et al. 2017

Journal of Food Engineering

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show abstract

“…Результати досліджень, проведених in vitro, свідчать про різні його біологічні ефекти, зокрема протизапальний, антиоксидантний та судинорозширювальний [2]. Завдяки таким властивостям кверцетин успішно застосовується при хворобах печін-ки, покращуючи біомаркери тканини цього органу при ураженнях різного характеру [3,4], а його екстракти, одержані з лікарських рослин, мають виражену гепатопротекторну й жовчогінну дію та впливають на склад жовчі [5,6]. Як і більшість флавоноїдів, кверцетин має низьку біодоступність для організму, що обмежує вивчення особливостей його ефектів in vivo.…”

Section: вступunclassified

“…* Р<0,05; ** Р<0,01; *** Р<0,001 порівняно з контролем Зовнішньосекреторна функція печінки щурів при дії корвітину дослідах in vivo, в тому числі й на жовчосе-креторну функцію печінки. Проте спирто-вий екстракт Azadirachta indica, до складу якого він входить, майже удвічі збільшував об'єм виділеної жовчі у щурів [6]. Секреція останньої відбувається завдяки осмотично залежному надходженню води в жовчні кана-лікули, за яке відповідає активний транспорт осмотично активних речовин.…”

Section: результати та їх обговоренняunclassified

Exocrine Function of the Liver in Rats With Exposure to Cоrvitin

Vovkun¹,

Yanchuk²,

Shtanova³

et al. 2016

Fiziol Zh

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“…Іn particular, it was found that this flavonoid protects hepatocytes from chronic ethanol toxicity by modulation of mitochondria function and liver enzymes activity [14]. To the best of our knowledge, there is no sufficient data regarding choleretic effect of purified QUE in vіvо, however, it has been reported that herbal extracts containing this flavonoid stimulate bile secretion [15]. We identified earlier that corvitin evokes an increase of bile volume in rats: after injection of 2.5, 5 and 10 mg/kg flavonoid total bile volume was increased by 20.9% (P < 0.01), 30% (P < 0.001) and 20.4% (P < 0.05), respectively [16].…”

Section: Resultsmentioning

confidence: 99%

The features of bile acids exchange in rats under the influence of corvitin

Vovkun¹,

Yanchuk²,

Shtanova³

et al. 2017

Ukr.Biochem.J.

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Corvitin is a soluble form of quercetin (QUE) and its effects are based on the ability to inhibit the activity of 5-lipoxygenase and to block the formation of leukotrienes. Corvitin increases bloodflow inE nzyme 5-lipoxygenase (5-LO) plays an essential role in the biosynthesis of leukotrienes (LTs). Leukotriene LTB4 is a potent chemotactic and chemokinetic agent for a variety of leukocytes, while cysteinyl leukotrienes C4, D4 and E4 regulate vascular permeability and smooth muscle contraction. These compounds have vasoconstrictor properties and cause prolonged increased pressure in large blood vessels and capillaries. Inhibitors of LT synthesis have been hypothesized to possess therapeutic potential for the treatment of asthma, allergic disorders and other diseases [1]. Physiological effects of LTs on the liver are not stu died enough. It was shown that the addition of LTD4 to liver perfusate resulted in a decrease of portal blood flow, bile flow and bile acids (BAs) release [2,3]. Specific blockers of 5LO activity do not affect the synthesis of other arachidonic acid metabolites -prostaglandins and thromboxane [4].Formation of bile by hepatocytes is an important way of removing potentially harmful exogenous © 2017 Vovkun T. V. et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. lipophilic substances and endogenous compounds such as bilirubin and cholesterol [5]. Bile contains ВAs, which are synthesized in the liver from cholesterol. These molecules ensure the stability of bile colloidal system, dispersion and absorption of dietary fats in the intestine, activation of pancreatic lipase, intensification of intestinal motility [6].Plant polyphenol quercetin (QUE) attracts attention due to a wide range of positive effects on human health including hepatoprotective effect [7]. But its impact on bile formation and secretion has not been studied. It is known that QUE effects depend on applied doses, redox status of target cells and metabolites synthesis [8]. Despite the high effectiveness of QUE its bioavailability is very low. This complicates the investigation of QUE effects in vivo. In this study we used a water-soluble form of QUE corvitin at different doses. Corvitin is the inhibitor of 5LO, it is natural compound without side effects, that has been used to treat cardiac disorders [9]. Previously we found that this flavonoid modulates gastric sedoi: https://doi.org/10.15407/ubj89.05.106

show abstract

Increased bile flow rate and altered composition of bile induced by ethanolic leaf extract of Azadirachta indica (neem) in rats

Cited by 8 publications

References 0 publications

In vitro digestion of pectin- and mango-enriched diets using a dynamic rat stomach-duodenum model

In vitro digestion of pectin- and mango-enriched diets using a dynamic rat stomach-duodenum model

Exocrine Function of the Liver in Rats With Exposure to Cоrvitin

The features of bile acids exchange in rats under the influence of corvitin

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