Increased Autolysis of<i>μ</i>-Calpain in Skeletal Muscles of Chronic Alcohol-Fed Rats

Gritsyna, Yulia; Salmov, N. N.; Bobylev, A. G.; Уланова, А. Д.; Kukushkin, N. I.; Podlubnaya, Z. A.; Vikhlyantsev, I. M.

doi:10.1111/acer.13476

Cited by 16 publications

(17 citation statements)

References 49 publications

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“…Indoles are products of tryptophan catabolism and ligands for the aryl hydrocarbon receptor (AHR), which is necessary for intestinal IL-22 production (56). Metabolic profiling in some of the included studies in our paper confirmed lower levels of indoles in a dysbiotic subpopulation (17) and reduced tryptophan biosynthesis (20,30).…”

Section: Sourcesupporting

confidence: 63%

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Changes in the composition of the human intestinal microbiome in alcohol use disorder: a systematic review

Litwinowicz

Choroszy

Waszczuk

2019

The American Journal of Drug and Alcohol Abuse

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Background: A growing body of evidence highlights the role of the intestine in the development of various alcohol use disorder (AUD) complications. The intestinal microbiome has been proposed as an essential factor in mediating the development of AUD complications such as alcoholic liver disease. Objectives: To provide a comprehensive description of alcohol-induced intestinal microbiome alterations. Methods: We conducted a systematic review of studies investigating the effect of alcohol on the intestinal microbiome using the PRISMA checklist. We searched the Medline database on the PubMed platform for studies determining the effect of alcohol on microbiota in individuals with AUD. The manual search included references of retrieved articles. Only human studies examining the intestinal bacterial microbiome using 16S ribosomal RNA sequencing were included. Data comparing relative abundances of bacteria comprising intestinal microbiota was extracted. Results: We retrieved 17 studies investigating intestinal microbiome alterations in individuals with AUD. Intestinal microbiome alterations in individuals with AUD included depletion of Akkermansia muciniphila and Faecalibacterium prausnitzii and an increase of Enterobacteriaceae. At the phylum level, a higher abundance of Proteobacteria and lower of Bacteroidetes were found. Mixed results regarding Bifidobacterium were obtained. Several species of short-chain fatty acids producing bacteria had a lower abundance in individuals with alcohol use disorder. Conclusion: Intestinal microbiome alterations associated with dysbiosis in individuals with AUD are generally consistent across studies, making it a promising target in potential AUD complications treatment. ARTICLE HISTORY

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Section: Sourcesupporting

confidence: 63%

“…Studies included in this section scored on average 6 points in the NOS. Alcoholic cirrhosis was characterized by an increase of Enterobacteriaceae (20,26) and a reduction of Lachnospiraceae (20,26) at the family level and an increase in Enterobacter (20,26) at the genus level (Table 4).…”

Section: Differences Between Individuals With Alcoholic and Nonalcohomentioning

confidence: 99%

Changes in the composition of the human intestinal microbiome in alcohol use disorder: a systematic review

Litwinowicz

Choroszy

Waszczuk

2019

The American Journal of Drug and Alcohol Abuse

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“…Однако имеются косвенные указания на то, что увеличение степени фосфорилирования титина сопровождается повышенной протеолитической деградацией этого белка. Аналогичные изменения уровня фосфорилирования Т1 и Т2, сопровождающиеся повышенным протеолизом интактного титина, были обнаружены при развитии алкоголь-индуцированной атрофии в икроножной и камбаловидной мышцах крыс [3]. Имеются также данные, что увеличение степени фосфорилирования титина в четырехглавой мышце бедра пациентов c синдромом Элеpcа-Данло сопровождалось снижением содержания этого белка [23].…”

Section: р е з у л ь т а т ы о ц е н к и о б ъ е м а с к е л е т н ы unclassified

“…В литературе встречаются единичные экспериментальные исследования, посвященные изменениям основных сократительных белков скелетной мышцы на фоне хронической алкогольной интоксикации. В частности, получены данные о снижении содержания гигантских саркомерных белков титина и небулина относительно содержания тяжелых цепей миозина в мышцах задних конечностей хронически алкоголизированных крыс [3,4]. Обсуждается участие титина и небулина в регуляции взаимодействия основных сократительных белков скелетной мышцы -актина и миозина -и мышечного сокращения в целом.…”

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Change in the content of titin and nebulin and their phosphorylation level in the quadriceps femoris muscle in chronic alcoholic myopathy

Zinovyeva

Самхаева

Vikhlyantsev

et al. 2019

Nevrologiâ, nejropsihiatriâ, psihosomatika

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Цель исследования-оценка структурно-функционального состояния скелетных мышц бедра, а также изменений в содержании и уровне фосфорилирования белков саркомерного цитоскелета титина и небулина у пациентов с хронической алкогольной интоксикацией.

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“…Titin is supposed to "…sense the mechanical stimuli and transform them into biochemical signals…" [34]. Great attention is now focused on the study of titin role in cardiomyopathies and skeletal muscle diseases [2,[39][40][41][42][43][44][45][46][47].…”

Section: Electrophoresis -Life Sciences Practical Applications 46mentioning

confidence: 99%

Peculiarities of SDS-PAGE of Titin/Connectin

Vikhlyantsev¹,

Podlubnaya²

2018

Electrophoresis - Life Sciences Practical Applications

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Titin (also known as connectin) is a giant elastic protein of striated and smooth muscles of vertebrates. The molecular weight of its isoforms is 3.0-3.7 MDa in striated muscles and 0.5-2.0 MDa in smooth muscles. Titin was discovered 40 years ago using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). At the present time, this method has not lost its relevance but has undergone a number of modifications that improve visualization of giant titin isoforms in the gel. This chapter provides historical insights into the technical aspects of the electrophoresis methods used to identify titin and its isoforms. We focus on the peculiarities of the technique because of which titin molecules remain intact and its high molecular weight isoforms can be visualized. Electrophoretic testing of changes in titin content in muscles can be used in medical practice to diagnose pathological processes and evaluate effective approaches to their correction. Figure 7. Increased proteolysis of titin and reduction of T1 content in m. Soleus of post-apoplectic patient. Vertical agarose-strengthened 2.3% polyacrylamide gel (8.0 × 10.0 × 0.10 cm) was used to separate titin isoforms. (1) Human m. soleus (control); (2) m. soleus of post-apoplectic patient.

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Increased Autolysis ofμ-Calpain in Skeletal Muscles of Chronic Alcohol-Fed Rats

Cited by 16 publications

References 49 publications

Changes in the composition of the human intestinal microbiome in alcohol use disorder: a systematic review

Changes in the composition of the human intestinal microbiome in alcohol use disorder: a systematic review

Change in the content of titin and nebulin and their phosphorylation level in the quadriceps femoris muscle in chronic alcoholic myopathy

Peculiarities of SDS-PAGE of Titin/Connectin

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