Increased arterial collagen synthesis in experimental canine atherosclerosis

McCullagh, K.G.; Ehrhart, L A

doi:10.1016/0021-9150(74)90040-9

Cited by 71 publications

(14 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydroxyproline and proline were resolved by ionexchange chromatography over AG 50W-X6 resin (BioRad, Richmond, Calif.) as previously described. 24 Collagen and noncollagenous protein syntheses were calculated from the radioactivity in the hydroxyproline and proline fractions as described. 23 The units of collagen and total protein synthesis are expressed as disintegrations per minute and were calculated to be proportional to the quantities of protein synthesized.…”

Section: Collagen and Noncollagenous Protein Synthesismentioning

confidence: 99%

Basic fibroblast growth factor in the extracellular matrix suppresses collagen synthesis and type III procollagen mRNA levels in arterial smooth muscle cell cultures.

Majors

Ehrhart

1993

Arterioscler Thromb

View full text Add to dashboard Cite

To determine the effects of an intact extracellular matrix on collagen synthesis, arterial smooth muscle cells (SMCs) were plated sparsely on a cell-free, SMC-derived matrix and examined the following day. Collagen synthesis during a 5-hour incubation by cells on the matrix was reduced to 67% of the control values obtained from cultures on plastic. Total protein synthesis was unaffected. Treatment of the matrix with heparitinase to remove basic fibroblast growth factor (bFGF) before seeding the SMCs abolished the inhibitory effect of the matrix on collagen synthesis. The inhibitory effect was also eliminated by treating the matrix with a neutralizing polyclonal antibody directed against bFGF. Collagen synthesis by SMC cultures grown in wells coated with purified bFGF was only 61% that of control cultures, whereas total protein synthesis remained unchanged. Slot-blot analysis revealed that the relative message level for orl(III) procollagen was reduced in cultures grown on the preexisting matrix or on plastic precoated with bFGF, whereas the al(I) procollagen message was unaffected. These results demonstrate the ability of the extracellular matrix to modulate the synthesis of collagen by arterial SMCs and indicate that bFGF in the matrix is responsible for these effects. (Arteriosclerosis and Thrombosis 1993; 13:680-686) KEY WORDS • collagen • smooth muscle cells • basic fibroblast growth factor • extracellular matrixC ells in vivo are in contact with an insoluble matrix composed of collagens, elastin, proteoglycans, noncollagenous glycoproteins, and a variety of adherent nonstructural components including growth factors. This extracellular matrix has the capacity to modulate the behavior and phenotype of cells both in vivo and in vitro. Several types of cultured cells have been shown to respond to the substratum onto which they are plated by altering their adhesion, growth, morphology, and protein synthesis patterns.Numerous studies have shown that preformed matrices, when used as a substratum for cultured cells, may have significant effects on cell proliferation.1 " 3 In addition, the substratum on which cells are plated can have specific effects on the synthesis of extracellular matrix components.4 " 6 Previous studies in our laboratory have demonstrated that preconfluent smooth muscle cells (SMCs) grown on fibronectin synthesize significantly less collagen and fibronectin than control SMCs, whereas noncollagenous protein synthesis was unaltered. 7 In contrast, both collagen and fibronectin syn-

show abstract

Section: Collagen and Noncollagenous Protein Synthesismentioning

confidence: 99%

Basic fibroblast growth factor in the extracellular matrix suppresses collagen synthesis and type III procollagen mRNA levels in arterial smooth muscle cell cultures.

Majors

Ehrhart

1993

Arterioscler Thromb

View full text Add to dashboard Cite

show abstract

“…Recently, workers in several laboratories have reported increased biosynthesis of collagen in experimental atherosclerosis (3,7,8). The availability in this laboratory of methods for measuring a number of markers of collagen biosynthesis has made it possible to determine the effects of hypertension on collagen biosynthesis in blood vessels.…”

mentioning

confidence: 99%

Increased Collagen Synthesis in Blood Vessels of Hypertensive Rats and Its Reversal by Antihypertensive Agents

Ooshima

Fuller

Cardinale

et al. 1974

Proc. Natl. Acad. Sci. U.S.A.

144

View full text Add to dashboard Cite

Collagen synthesis is increased in the aortas, mesenteric arteries, and to a lesser extent, in the hearts of rats either made hypertensive with desoxycorticosterone acetate-salt or that are spontaneously hypertensive. Several markers of collagen biosynthesis were shown to be increased, including prolyl hydroxylase (EC 1.14.11.2; proline, 2-oxoglutarate dioxygenase), prolyl hydroxylaserelated antigen, total collagen content, and the incorporation of [3Hiproline into total protein and into collagen.The antihypertensive agents chlorothiazide and reserpine, when administered before the onset of hypertension in the rats treated with desoxycorticosterone acetate-salt, prevented or diminished the increase in collagen biosynthesis. When reserpine was given after the onset of hypertension, prolyl hydroxylase activity was decreased concomitant with the decrease in blood pressure. Treatment with reserpine is particularly effective in diminishing arterial prolyl hydroxylase activity.

show abstract

“…(c) Third method: a chromatographic procedure on Dowex 50X 8 in hydrochloric acid solution according to McCullagh and Ehrhart [4]. This procedure was found very reliable because it allows a quantitative separation of hydroxyproline and proline but the large volumes of 1 N HC1 which have to be evaporated before these samples can be counted are troublesome.…”

Section: Methodsmentioning

confidence: 99%