Increased Apolipoprotein E Gene Expression and Protein Concentration in Lung Cancer Tissue Do Not Contribute to the Clinical Assessment of Non-small Cell Lung Cancer Patients

Trošt, Zoran; Marc, Janja; Sok, Mihael; Černe, Darko

doi:10.1016/j.arcmed.2008.06.009

Cited by 18 publications

(14 citation statements)

References 14 publications

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“…In a group of stages IA and IIIA lung cancer patients, Trost et al [17] reported that there was no association between patients' survival and ApoE gene expression or protein concentration in early stage of lung cancer tissue. In the current cohort of patients with lung adenocarcinoma and MPE over-expression of ApoE protein predicts poor overall survival.…”

Section: Discussionmentioning

confidence: 98%

“…Once secreted, ApoE has been shown to act as an autocrine or paracrine growth factor to influence the microenvironment, which can contribute to carcinogenesis [16]. In human lung cancer, for example, the levels of ApoE gene expression and protein expression were significantly higher in cancer tissue than in adjacent non-cancer tissue [17]. The exact role that ApoE protein plays in human lung carcinogenesis has, however, not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Apolipoprotein E expression promotes lung adenocarcinoma proliferation and migration and as a potential survival marker in lung cancer

Chen

Lai

et al. 2011

Lung Cancer

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Apolipoprotein E expression promotes lung adenocarcinoma proliferation and migration and as a potential survival marker in lung cancer

Chen

Lai

et al. 2011

Lung Cancer

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“…A previous study of ApoE was concerned with the association between ApoE and hyperlipidemia or related cardiovascular and cerebrovascular diseases (15), although in recent years, with more in-depth study, an increasing amount of research has been concerned with the role of ApoE in tumors. The expression of ApoE has been reported to be increased in a variety of tumors (16). Grant (17) reported that the upregulation of ApoE is a risk factor for prostate cancer.…”

Section: Discussionmentioning

confidence: 99%

Expression and clinical significance of complement C3, complement C4b1 and apolipoprotein E in pancreatic cancer

Chen

Zhen

et al. 2013

Oncology Letters

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Pancreatic cancer (PC) remains a devastating disease with a five-year survival rate of <5%. The difficulty in making an early diagnosis and the frequent occurrence of metastasis are important reasons for this poor prognosis. In China, the incidence of PC has been increasing steadily. Therefore, the present study aimed to identify effective markers in the early and advanced stages of PC. The expression levels of complement C3, complement C4b1 and apolipoprotein E (ApoE) in the various stages of PC were assessed by immunohistochemistry, RT-PCR and western blotting. Additionally, the statistical significance of the results was analyzed. The expression levels of complement C3, complement C4b1 and apoE were higher in PC compared with normal pancreatic tissues. No correlations were observed between complement C3 and tumor TNM staging or lymph node metastasis. However, complement C4b1 and apoE were markedly correlated with tumor TNM staging and lymph node metastasis. Complement C3 may be used as a marker for the diagnosis of early-stage PC, while complement C4b1 and apoE are closely correlated with tumor development, reflecting the biological behavior of PC, and thus may be used as diagnostic markers of advanced PC.

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“…RNA was isolated from tissue with RNeasy Mini kit (Qiagen, Hilden, Germany) and reverse transcribed to cDNA using the High-Capacity cDNA Archive kit (Applied Biosystems, Foster, CA, USA) as previously described (23). …”

Section: Methodsmentioning

confidence: 99%

“…GUSB was selected as the most appropriate internal control among the 11 candidates available on TaqMan Human Endogenous Control Plate (Applied Biosystems), as previously described (23). The average PEMT and GUSB PCR amplification efficiency (E) was considered in calculating relative PEMT mRNA quantities (RQ) using the following equation: RQ=(1+E) exp (−ΔΔCt).…”

Section: Methodsmentioning

confidence: 99%

Increased phosphatidylethanolamine N-methyltransferase gene expression in non-small-cell lung cancer tissue predicts shorter patient survival

et al. 2014

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Lipid mobilization is of great importance for tumor growth and studies have suggested that cancer cells exhibit abnormal choline phospholipid metabolism. In the present study, we hypothesized that phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is increased in non-small-cell lung cancer (NSCLC) tissues and that increased gene expression acts as a predictor of shorter patient survival. Forty-two consecutive patients with resected NSCLC were enrolled in this study. Paired samples of lung cancer tissues and adjacent non-cancer lung tissues were collected from resected specimens for the estimation of PEMT expression. SYBR Green-based real-time polymerase chain reaction was used for quantification of PEMT mRNA in lung cancer tissues. Lipoprotein lipase (LPL) and fatty acid synthase (FASN) activities had already been measured in the same tissues. During a four-year follow-up, 21 patients succumbed to tumor progression. One patient did not survive due to non-cancer reasons and was not included in the analysis. Cox regression analysis was used to assess the prognostic value of PEMT expression. Our findings show that elevated PEMT expression in the cancer tissue, relative to that in the adjacent non-cancer lung tissue, predicts shorter patient survival independently of standard prognostic factors and also independently of increased LPL or FASN activity, the two other lipid-related predictors of shorter patient survival. These findings suggest that active phosphatidylcholine and/or choline metabolism are essential for tumor growth and progression.

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Increased Apolipoprotein E Gene Expression and Protein Concentration in Lung Cancer Tissue Do Not Contribute to the Clinical Assessment of Non-small Cell Lung Cancer Patients

Cited by 18 publications

References 14 publications

Apolipoprotein E expression promotes lung adenocarcinoma proliferation and migration and as a potential survival marker in lung cancer

Apolipoprotein E expression promotes lung adenocarcinoma proliferation and migration and as a potential survival marker in lung cancer

Expression and clinical significance of complement C3, complement C4b1 and apolipoprotein E in pancreatic cancer

Increased phosphatidylethanolamine N-methyltransferase gene expression in non-small-cell lung cancer tissue predicts shorter patient survival

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