Increased Aneuploidy Rate in Sperm With Fragmented DNA as Determined by the Sperm Chromatin Dispersion (SCD) Test and FISH Analysis

Muriel, Lourdes; Goyanes, Vicente; Segrelles, Enrique; Gosálvez, Jaime; Álvarez, Juan G.; Fernández, José Luís

doi:10.2164/jandrol.106.000067

Cited by 72 publications

(43 citation statements)

References 62 publications

(81 reference statements)

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“…In addition, mis-segregated chromosomes may themselves trigger the apoptotic process [Dobles et al 2000]. Sperm DNA damage may be part of a genomic surveillance mechanism to control or maintain normal production of spermatozoa and to inactivate and reject spermatozoa with altered or an unbalanced genome [Muriel et al 2007]. This theory might explain the strong relationship between aneuploidy and sperm DNA damage that we observed in our study, and as reported by others [Carrell et al 2003;Muriel et al 2007].…”

Section: Discussionsupporting

confidence: 82%

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

Moskovtsev¹,

Alladin²,

Lo³

et al. 2012

Systems Biology in Reproductive Medicine

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Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates in spermatozoa are elevated at the testicular level in infertile patients compared to ejaculates of normal controls. However, essential data regarding aneuploidy rates between ejaculated and testicular spermatozoa in the same individuals is lacking. The purpose of our study was to compare aneuploidy rates at the testicular and post-testicular level from the same patients with persistently high sperm DNA damage. Ejaculates and testicular biopsies were obtained from eight patients with persistently high DNA damage (>30%). Both ejaculated and testicular samples were analyzed for sperm DNA damage and sperm aneuploidy for chromosomes 13, 18, 21, X, and Y. In addition, semen samples from ten normozoospermic men presenting for fertility evaluation served as a control group. A strong correlation between the alteration of spermatogenesis and chromatin deterioration was observed in our study. In the same individuals, testicular samples showed a significantly lower DNA damage compared to ejaculated spermatozoa (14.9% ± 5.0 vs. 40.6% ± 14.8, P < 0.05), but significantly higher aneuploidy rates for the five analyzed chromosomes (12.41% ± 3.7 vs. 5.77% ± 1.2, P < 0.05). While testicular spermatozoa appear favourable for ICSI in terms of lower DNA damage, this potential advantage could be offset by the higher aneuploidy rates in testicular spermatozoa.

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Section: Discussionsupporting

confidence: 82%

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

Moskovtsev¹,

Alladin²,

Lo³

et al. 2012

Systems Biology in Reproductive Medicine

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show abstract

“…Moreover, a systematic review and meta-analysis of IVF and ICSI studies shows that sperm DNA damage is associated with a significant increase in the rate of pregnancy loss after IVF and ICSI [36]. The increased rate of failed embryos and pregnancy loss associated with male factor infertility and sperm DNA damage in IVF and ICSI may be due to sperm chromosomal aneuploidy but this has not been demonstrated conclusively [7,28]. It has been suggested that the process of sperm selection during ICSI in humans may lessen the potential undesirable effects of sperm DNA damage on fertility outcomes [15].…”

Section: Discussionmentioning

confidence: 99%

High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

Das

Alhathal

San-Gabriel

et al. 2013

J Assist Reprod Genet

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Background Sperm DNA damage is associated with male infertility, lower pregnancy rates and pregnancy loss. Objective The primary aim of our study was to evaluate the prevalence of sperm DNA damage in younger and older men with normozoospermia. Design, Setting and Participants We obtained semen from 277 consecutive non-azoospermic men presenting for sperm DNA testing. Outcome Measurements and Statistical Analysis The main outcome measures included sperm % DNA fragmentation index (%DFI, using sperm chromatin structure assay), sperm concentration, motility and morphology, and, paternal age.Results and Limitations Sperm % DFI was positively correlated with paternal age (r=0.20, P<0.001) and inversely correlated % progressive motility (r=−0.16, P=0.01). Sperm %DFI was significantly higher in older (≥40 years) compared to younger (<40 years) normozoospermic men (17±13 vs. 12±8, respectively P=0.008), whereas, sperm concentration, progressive motility and morphology were not significantly different in these two groups. Moreover, the prevalence of high levels of sperm DNA damage (>30 % DFI) was significantly higher in older compared to younger normozoospermic men (17 % vs. 3 %, respectively, P<0.001). Conclusion The data indicate that a conventional semen analysis can often fail to detect a defect in spermatogenesis (high %DFI) in older men and suggest that infertile couples with advanced paternal age, including those with normal semen parameters, should consider sperm DNA testing as part of the couple evaluation.

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“…In assisted reproduction programs, several studies show that high levels of sperm DNA fragmentation are related to lower pregnancies rates either natural or using IUI, IVF or ICSI procedures [54][55][56][57] and higher aneuploidies rates in embryos [52,58,59]. Greco et al [60] reported 29 ICSI cycles in which the percentage of DNA-fragmented spermatozoa, detected by TUNEL assay, was >15%; only two pregnancies and no births were obtained.…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

Ferreyra¹

2014

JFIV Reprod Med Genet

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Purpose: To evaluate the levels of DNA fragmentation regarding to spermatozoa morphology in male infertility patients attending Andrology Laboratory at fertility center.Methods: Semen samples of 196 patients were analyzed using computer-assisted semen analysis (CASA). Sperm DNA fragmentation was measured by the sperm chromatin dispersion test. According to the values of sperm morphology, the groups were conformed by teratozoospermic (105 patients) and normozoospermic (91 patients) men.Results: Subjects' ages ranged from 21 to 68 years. Teratozoospermic men were older that the normozoospermic men (39.72 ± 7.86 vs. 36.57 ± 6.29 years; P<0.05). Values of pH were similar in both evaluated groups (P:NS). Parameters of volume, concentration, motility and vitality in teratozoospermic men were significantly lower compared to those normozoospermic patients (P<0.05). High levels of DNA fragmentation were observed in those patients with abnormal sperm morphology compared to normozoospermic men (44.31 ± 7.52 vs. 34.92 ± 5.89; P<0.05). Conclusions:Men with abnormal spermatozoa morphology showed high levels of DNA fragmentation. These significantly high percentages of sperm DNA damage will be an additional factor that drastically reduces the possibility of success in these infertile men.

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Increased Aneuploidy Rate in Sperm With Fragmented DNA as Determined by the Sperm Chromatin Dispersion (SCD) Test and FISH Analysis

Cited by 72 publications

References 62 publications

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

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