Increased Amylopectin Content Potential in Corn Grains of Quality Protein Maize (QPM)

Edy,; Numba, Sudirman; Ibrahim, Bona Malik

doi:10.1088/1755-1315/334/1/012011

Cited by 2 publications

(2 citation statements)

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“…In the world in general, normal maize is more cultivated in terms of production amount and area and is mostly used for food and feed. On the other hand, waxy maize, known as sticky or glutinous maize, is a unique type of cultivated maize, the immature ears of which are mainly used for food in East and South-East Asian countries [ 8 , 9 ]. Starch of the normal maize endosperm is made up of approximately 25% amylose and 75% amylopectin [ 1 ], whereas starch of the waxy maize endosperm consists of over 99% amylopectin.…”

Section: Introductionmentioning

confidence: 99%

Association Mapping of Amylose Content in Maize RIL Population Using SSR and SNP Markers

Jin

Park

Jang

et al. 2023

Plants

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The ratio of amylose to amylopectin in maize kernel starch is important for the appearance, structure, and quality of food products and processing. This study aimed to identify quantitative trait loci (QTLs) controlling amylose content in maize through association mapping with simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers. The average value of amylose content for an 80-recombinant-inbred-line (RIL) population was 8.8 ± 0.7%, ranging from 2.1 to 15.9%. We used two different analyses—Q + K and PCA + K mixed linear models (MLMs)—and found 38 (35 SNP and 3 SSR) and 32 (29 SNP and 3 SSR) marker–trait associations (MTAs) associated with amylose content. A total of 34 (31 SNP and 3 SSR) and 28 (25 SNP and 3 SSR) MTAs were confirmed in the Q + K and PCA + K MLMs, respectively. This study detected some candidate genes for amylose content, such as GRMZM2G118690-encoding BBR/BPC transcription factor, which is used for the control of seed development and is associated with the amylose content of rice. GRMZM5G830776-encoding SNARE-interacting protein (KEULE) and the uncharacterized marker PUT-163a-18172151-1376 were significant with higher R2 value in two difference methods. GRMZM2G092296 were also significantly associated with amylose content in this study. This study focused on amylose content using a RIL population derived from dent and waxy inbred lines using molecular markers. Future studies would be of benefit for investigating the physical linkage between starch synthesis genes using SNP and SSR markers, which would help to build a more detailed genetic map and provide new insights into gene regulation of agriculturally important traits.

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Section: Introductionmentioning

confidence: 99%

Association Mapping of Amylose Content in Maize RIL Population Using SSR and SNP Markers

Jin

Park

Jang

et al. 2023

Plants

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“…Waxy maize, popularly known as 'sticky' maize or 'glutinous' maize, possesses 95-100% amylopectin compared to 70-75% in traditional maize 2,3 . Immature waxy cobs and dried grains are an essential part of the human diet in East and South-East Asian countries 4,5 . It is also used as vegetable, and various breakfast and snack items; and consumed as staple food by various ethnic groups 6 .…”

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confidence: 99%

Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes

Talukder

Muthusamy

Chhabra

et al. 2022

Sci Rep

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Waxy maize rich in amylopectin has emerged as a preferred food. However, waxy maize is poor in lysine and tryptophan, deficiency of which cause severe health problems. So far, no waxy hybrid with high lysine and tryptophan has been developed and commercialized. Here, we combined recessive waxy1 (wx1) and opaque2 (o2) genes in the parental lines of four popular hybrids (HQPM1, HQPM4, HQPM5, and HQPM7) using genomics-assisted breeding. The gene-based markers, wx-2507F/RG and phi057 specific for wx1 and o2, respectively were successfully used to genotype BC1F1, BC2F1 and BC2F2 populations. Background selection with > 100 SSRs resulted in recovering > 94% of the recurrent parent genome. The reconstituted hybrids showed 1.4-fold increase in amylopectin (mean: 98.84%) compared to the original hybrids (mean: 72.45%). The reconstituted hybrids also showed 14.3% and 14.6% increase in lysine (mean: 0.384%) and tryptophan (mean: 0.102%), respectively over the original hybrids (lysine: 0.336%, tryptophan: 0.089%). Reconstituted hybrids also possessed similar grain yield (mean: 6248 kg/ha) with their original versions (mean: 6111 kg/ha). The waxy hybrids with high lysine and tryptophan assume great significance in alleviating malnutrition through sustainable and cost-effective means. This is the first report of development of lysine and tryptophan rich waxy hybrids using genomics-assisted selection.

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Increased Amylopectin Content Potential in Corn Grains of Quality Protein Maize (QPM)

Cited by 2 publications

References 1 publication

Association Mapping of Amylose Content in Maize RIL Population Using SSR and SNP Markers

Association Mapping of Amylose Content in Maize RIL Population Using SSR and SNP Markers

Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes

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