Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An <i>in vitro</i> study

Tateshima, Satoshi; Kaneko, Naoki; Yamada, Masahiro; Duckwiler, Gary; Viñuela, Fernando; Ogawa, Takahiro

doi:10.1002/jbm.a.36304

Cited by 11 publications

(8 citation statements)

References 22 publications

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“…Similarly, hydrophilic zirconia and Co-Cr alloy enhances the initial behavior and response of osteoblasts compared to hydrophobic zirconia and Co-Cr alloy, respectively [41,[43][44][45][46]. Hydrophilic titanium also effectively attracts and retains proteins and other cell types such as endothelial cells [47,48]. There are few reports of the effect of hydrophilicity/hydrophobicity of resinous materials.…”

Section: Discussionmentioning

confidence: 99%

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Sugita

Okubo

Saita

et al. 2020

IJMS

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Poly(methyl methacrylate) (PMMA)-based bone cement, which is widely used to affix orthopedic metallic implants, is considered bio-tolerant but lacks osteoconductivity and is cytotoxic. Implant loosening and toxic complications are significant and recognized problems. Here we devised two strategies to improve PMMA-based bone cement: (1) adding 4-methacryloyloxylethyl trimellitate anhydride (4-META) to MMA monomer to render it hydrophilic; and (2) using tri-n-butyl borane (TBB) as a polymerization initiator instead of benzoyl peroxide (BPO) to reduce free radical production. Rat bone marrow-derived osteoblasts were cultured on PMMA-BPO, common bone cement ingredients, and 4-META/MMA-TBB, newly formulated ingredients. After 24 h of incubation, more cells survived on 4-META/MMA-TBB than on PMMA-BPO. The mineralized area was 20-times greater on 4-META/MMA-TBB than PMMA-BPO at the later culture stage and was accompanied by upregulated osteogenic gene expression. The strength of bone-to-cement integration in rat femurs was 4- and 7-times greater for 4-META/MMA-TBB than PMMA-BPO during early- and late-stage healing, respectively. MicroCT and histomorphometric analyses revealed contact osteogenesis exclusively around 4-META/MMA-TBB, with minimal soft tissue interposition. Hydrophilicity of 4-META/MMA-TBB was sustained for 24 h, particularly under wet conditions, whereas PMMA-BPO was hydrophobic immediately after mixing and was unaffected by time or condition. Electron spin resonance (ESR) spectroscopy revealed that the free radical production for 4-META/MMA-TBB was 1/10 to 1/20 that of PMMA-BPO within 24 h, and the substantial difference persisted for at least 10 days. The compromised ability of PMMA-BPO in recruiting cells was substantially alleviated by adding free radical-scavenging amino-acid N-acetyl cysteine (NAC) into the material, whereas adding NAC did not affect the ability of 4-META/MMA-TBB. These results suggest that 4-META/MMA-TBB shows significantly reduced cytotoxicity compared to PMMA-BPO and induces osteoconductivity due to uniquely created hydrophilic and radical-free interface. Further pre-clinical and clinical validations are warranted.

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Section: Discussionmentioning

confidence: 99%

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Sugita

Okubo

Saita

et al. 2020

IJMS

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“…Thus, such surface texture-induced microenvironments with stagnated and concentrated blood and proteins may explain the successful, contact osteogenesis or osseointegration. The effects of hydrophilic titanium surfaces on the recruitment of blood, proteins, and cells have been reported extensively [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The present study has revealed that a similar effect can occur by surface texturing.…”

Section: Discussionmentioning

confidence: 99%

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

Kitajima,

Hirota,

Osawa

et al. 2023

Biomimetics

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The mechanisms underlying bone-implant integration, or osseointegration, are still incompletely understood, in particular how blood and proteins are recruited to implant surfaces. The objective of this study was to visualize and quantify the flow of blood and the model protein fibrinogen using a computational fluid dynamics (CFD) implant model. Implants with screws were designed with three different surface topographies: (1) amorphous, (2) nano-trabecular, and (3) hybrid meso-spikes and nano-trabeculae. The implant with nano-topography recruited more blood and fibrinogen to the implant interface than the amorphous implant. Implants with hybrid topography further increased recruitment, with particularly efficient recruitment from the thread area to the interface. Blood movement significantly slowed at the implant interface compared with the thread area for all implants. The blood velocity at the interface was 3- and 4-fold lower for the hybrid topography compared with the nano-topography and amorphous surfaces, respectively. Thus, this study for the first time provides insights into how different implant surfaces regulate blood dynamics and the potential advantages of surface texturization in blood and protein recruitment and retention. In particular, co-texturization with a hybrid meso- and nano-topography created the most favorable microenvironment. The established CFD model is simple, low-cost, and expected to be useful for a wide range of studies designing and optimizing implants at the macro and micro levels.

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“…We validated the appearance of a hydrophilic surface on Grade 4 implants and titanium disks because the majority of implants are constructed from grade 4 titanium [90,93]. The effect of UV treatment, such as the conversion to a hydrophilic surface, has been demonstrated for various surface types and materials, including type 2 commercially pure titanium, grade 5 titanium alloy, and zirconia [29,31,38,41,42,46,47,49,50,55,63,64,66,69,72,[95][96][97].…”

Section: Discussionmentioning

confidence: 99%

Optimization of blood and protein flow around superhydrophilic implant surfaces by promoting contact hemodynamics

et al. 2022

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Despite the widespread use of metallic implants in orthopedic surgery and dentistry, gaps remain in our understanding of the biology of bone-implant integration, or osseointegration. Fundamental questions exist about osseointegration, including whether bone-implant integration differs from natural bone healing, why bone formation initiates at the implant interface and concentrically distant from the implant surface, and why bone remodeling and resorption vary in different regions around the implant[1-3]. Although the design of implant screw threads has been refined from a mechanical perspec-tive for better initial implant stability, the biological impact of thread design has not been explored. Various implant surfaces have been developed to accelerate and enhance bone-implant integration, but the biological mechanisms underpinning these improvements have yet to be fully investigated .Ultraviolet (UV) light has recently been used to favorably modify implant surfaces [2,3,. For example, titanium surfaces, regardless of their surface texture, are hydrophobic (H 2 O contact angle ≥60°), and treating these surfaces with UV light converts them to a superhydrophilic state with a contact angle of ≤5° or 0° for the majority of currently used titanium surfaces [30,[51][52][53][54][55][56][57][58][59][60][61][62][63]. This UV-induced hydrophilic conversion also occurs with other implant materials including titanium alloy, chromium-cobalt alloy, and zirconia [29,38,39,41,42,44,45,47,[64][65][66][67][68]. These superhydrophilic implant surfaces promote cell attachment and increase the speed and percentage of bone coverage around the implants [2,3,9,30,32,34,[69][70][71][72]. Osteogenesis around superhydrophilic implants is concentrated at J Prosthodont Res. 2022; **(**):

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Increased affinity of endothelial cells to NiTi using ultraviolet irradiation: An in vitro study

Cited by 11 publications

References 22 publications

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

Optimization of blood and protein flow around superhydrophilic implant surfaces by promoting contact hemodynamics

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