Increased adipose tissue expression of IL‐18R and its ligand IL‐18 associates with inflammation and insulin resistance in obesity

Ahmad, Rasheed; Thomas, Reeby; Kochumon, Shihab; Sindhu, Sardar

doi:10.1002/iid3.170

Cited by 42 publications

(32 citation statements)

References 44 publications

(54 reference statements)

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“…Conclusions of the present study are supported by the work of Ahmad et al [25]. In their research, they determined the adipose tissue expression of IL-18R and IL-18 mRNA/protein in lean, overweight, and obese individuals with and without T2D, 15 each, using qRT-PCR, immunohistochemistry, and confocal microscopy.…”

Section: Discussionsupporting

confidence: 76%

Study of interleukin-18 ( IL18 ) and high sensitive C - reactive protein ( CRP ) in type 2 diabetes ( T2D ) with or without obesity

Kamel*

El-Baz

Emam

et al. 2020

Arab Journal of Nuclear Sciences and Applications

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Both diabetes and obesity are associated with chronic low-grade inflammation. IL-18 is a cytokine that stimulates various cell types and has pleiotropic functions. It is elevated in obesity, metabolic syndrome and type 2 diabetes (T2D).The hypothesis that serum level of IL-18 and high sensitive C-reactive protein can be used as markers for presence of chronic low-grade inflammation in type 2 diabetes more than in obesity per se was tested. The study recruited 80 subjects. They comprised 20 diabetic obese cases (DM-OB group), 20 diabetic non-obese cases (DM-NOB group), : 20 non-diabetic obese cases (NDM-OB group) and 20 non-diabetic non-obese subjects (NDM-NOB group). All participants were submitted to careful history taking, through clinical investigations and laboratory assessment. It was found that hs-CRP was significantly high in DMOB group (3.4±0.5 mg/dl) followed by NDM-OB group (2.9±0.4 mg/dl) then DM-NOB group (2.3±0.3 mg/dl) and least in NDM-NOB (0.8±0.3 mg/dl). It was also found that serum IL-18 was significantly highest in DMOB group (397.8±33.9 pg/mL) followed by NDM-OB (368.8±26.7 pg/mL) group followed by DM-NOB group (335.9±18.4 pg/mL) and least in NDM-NOB group (281.1±22.9 pg/mL). Serum IL-18 levels are directly correlated with BMI, waist circumference, hip circumference and waist/hip ratio in all the studied groups and with HbA1c in the diabetic groups. It could be concluded that Serum IL-18 and hs-CRP are related to diabetes and obesity. They are associated with dyslipidemia, poor glycemic control, insulin resistance and impaired liver and renal functions

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Section: Discussionsupporting

confidence: 76%

Study of interleukin-18 ( IL18 ) and high sensitive C - reactive protein ( CRP ) in type 2 diabetes ( T2D ) with or without obesity

Kamel*

El-Baz

Emam

et al. 2020

Arab Journal of Nuclear Sciences and Applications

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“…In addition to the risk factors of the metabolic syndrome, this syndrome has also been associated with a proinflammatory state [51]. The present study investigated the antiinflammatory properties of the microencapsulated probiotic blend by quantifying serum CRP levels.…”

Section: Discussionmentioning

confidence: 99%

Novel microencapsulated probiotic blend for use in metabolic syndrome: design and in-vivo analysis

Iqbal

Westfall

Prakash

2018

Artificial Cells, Nanomedicine, and Biotechnology

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The increasing prevalence of the metabolic syndrome has made it a medical issue that currently affects 1 in 5 Canadians. The metabolic syndrome is defined by risk factors that predispose an individual to diabetes and cardiovascular disease. Current forms of interventions have been inadequate as substantiated by the fact that the prevalence of metabolic syndrome has not reduced over the years. The objective of this study was to investigate the therapeutic benefits of a novel microencapsulated probiotic blend in treating the metabolic syndrome. Three probiotic strains were microencapsulated into alginate-polylysine-alginate (APA) microcapsules: L. rhamnosus NCIMB 6375, L. plantarum NCIMB 8826 and L. fermentum NCIMB 5221. From the results, it was observed that the microencapsulated probiotic blend significantly reduced serum total cholesterol, LDL cholesterol and triglyceride levels (reducing from 516 mg/dL to 379 mg/dL, 314 mg/dL to 231 mg/dL and 580 mg/dL to 270 mg/dL, respectively). In addition, the administration of the microencapsulated probiotic blend was found to favourably influence the gut microbiota, decreasing Firmicutes levels and increasing Bacteroidetes levels. Overall, this work demonstrates the potential a microencapsulated probiotic blend could have in targeting multiple risk factors of the metabolic syndrome; however, greater research is still needed.

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“…We and others have reported the increased adipose tissue IL-6 expression in obesity/T2D which could have consequences either as a circulating pleiotropic adipocytokine or as a local regulator of adipose inflammation and insulin action [15][16][17]. Other inflammatory mediators that can be elevated in obesity/T2D and promote metabolic inflammation include IL-18, IL-23, and IL-1β [18,19]. The chemotactic cytokines that direct leukocytic migration along concentration gradients are celled chemokines.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients

Sindhu

Kochumon

Thomas

et al. 2020

Cells

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Interferon regulatory factors (IRFs) are emerging as the metabolic transcriptional regulators in obesity/type-2 diabetes (T2D). IRF5 is implicated with macrophage polarization toward the inflammatory M1-phenotype, nonetheless, changes in the adipose expression of IRF5 in T2D and relationship of these changes with other markers of adipose inflammation remain unclear. Therefore, we determined the IRF5 gene expression in subcutaneous adipose tissue samples from 46 T2D patients including 35 obese (Body Mass Index/BMI 33.83 ± 0.42 kg/m 2 ) and 11 lean/overweight individuals (BMI 27.55 ± 0.46 kg/m 2 ) using real-time qRT-PCR. IRF5 protein expression was assessed using immunohistochemistry and confocal microscopy. Fasting plasma glucose, insulin, HbA1c, C-reactive protein, cholesterol, low-and high-density lipoproteins (LDL/HDL), and triglycerides were measured using commercial kits. IRF5 gene expression was compared with that of signature inflammatory markers and several clinico-metabolic indicators. The data (mean ± SEM) show the enhanced adipose IRF5 gene (p = 0.03) and protein (p = 0.05) expression in obese compared to lean/overweight diabetic patients. Adipose IRF5 transcripts in diabetic obese individuals associated positively with those of TNF-α, IL-18, IL-23A, CXCL8, CCL2, CCL7, CCR1/5, CD11c, CD68, CD86, TLR4/7/10, Dectin-1, FGL-2, MyD88, NF-κB, IRF3, and AML1 (p < 0.05). In diabetic lean/overweight subjects, IRF5 expression associated with BMI, body fat %age, glucose, insulin, homeostatic model assessment of insulin resistance (HOMA-IR, C-reactive protein (CRP), IL-5, and IL-1RL1 expression; while in all T2D patients, IRF5 expression correlated with that of IRF4, TLR2/8, and CD163. In conclusion, upregulated adipose tissue IRF5 expression in diabetic obese patients concurs with the inflammatory signatures and it may represent a potential marker for metabolic inflammation in obesity/T2D.

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Increased adipose tissue expression of IL‐18R and its ligand IL‐18 associates with inflammation and insulin resistance in obesity

Cited by 42 publications

References 44 publications

Study of interleukin-18 ( IL18 ) and high sensitive C - reactive protein ( CRP ) in type 2 diabetes ( T2D ) with or without obesity

Study of interleukin-18 ( IL18 ) and high sensitive C - reactive protein ( CRP ) in type 2 diabetes ( T2D ) with or without obesity

Novel microencapsulated probiotic blend for use in metabolic syndrome: design and in-vivo analysis

Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients

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