Increase recombinant antibody yields through optimizing vector design and production process in CHO cells

Synthetic enhancers including TFREs improve transgene expression in CHO cells

“…CHO cells are the most common host cells for the commercial production of RTPs. Different strategies have been used to increase the productivity and quality of protein in CHO expression systems [ 7 , 10 , 13 , 16 ]. Expression vectors are one of key elements that influence recombinant protein yield and quality; thus, optimization of expression vectors can greatly increase transgene expression levels [ 13 , 16 , 17 , 21 ].…”

“…Different strategies have been used to increase the productivity and quality of protein in CHO expression systems [ 7 , 10 , 13 , 16 ]. Expression vectors are one of key elements that influence recombinant protein yield and quality; thus, optimization of expression vectors can greatly increase transgene expression levels [ 13 , 16 , 17 , 21 ]. The promoter is the main component of the vector expression cassette, and optimization of promoter can improve targeting specificity and gene expression [ 34 , 35 ].…”

“…To satisfy the growing market requirement of biopharmaceuticals, the approaches such as gene modification, process optimization, expression vector and cell line modification are necessary to enable higher production capacity, higher product quality, and lower production costs [ [10] , [11] , [12] ]. Among of them, the modification of expression vector is a positive method to improve the yield of recombinant proteins, and expression vector cassette engineering and using chromatin-modifying elements can enhance the production level and stability of genes of interest (GOI) [ [13] , [14] , [15] , [16] , [17] ].…”

Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells

“…In industrial production, lower culture temperature significantly affected cell growth and cell productivity of CHO cells (Nguyen et al 2020 ; Yang et al 2022 ). For antibody expression, the decrease in temperature significantly affected N-glycosylation, showing less processed glycosylation structures in the stable region of mAbs and affecting degradation (Vergara et al 2018 ).…”

“…In our previous studies, some strategies have been committed to improve the expression of recombinant proteins in mammalian cells (Life Technologies, #A11557-01), and screen the efficient expression vector elements (Li et al 2022a;Wang and Guo 2020;Wang et al 2023;Yang et al 2022), construct the cell line for enhancing transgenic expression and long-term stability (Jia et al 2018) and optimize serum-free cell medium (Li et al 2022b;Xu et al 2023) and other new optimization strategies in cell culture were also explored (Lu et al 2023;Yang et al 2022). With the increasing demands for RTPs, more attentions should be paid to the quality attributes of RTPs besides increasing the yields.…”