Increase of Serum β-Glucuronidase Activity in Human Diabetes Mellitus

Miller, Benjamin F.; Keyes, F.P.; Curreri, P. William

doi:10.1001/jama.1966.03100030083020

Cited by 41 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although it is still not known why a deficiency of insulin causes an increase in serum Q-glucuronidase activity as demonstrated in the alloxan diabetic rabbit, Miller et al (7) suggested that the increased f3-glucuronidase activity can be explained by the operation of a noninsulin-sensitive pathway for glucose metabolism, such as the glucuronic acid pathway. The authors (10) also determined the amounts of glucuronic acid excreted into the urine, after oral administration of glucuronolactone to alloxan diabetic rabbits , to be less than that in the normal rabbits.…”

Section: Discussionmentioning

confidence: 99%

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Shioya

Ohishi

Kakimoto

1968

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

In a previous paper (1), the authors demonstrated that changes in serum Q-glucuroni dase activity paralleled blood sugar levels after the administration of glucose, epinephrine and insulin, while an inverse relationship was found with hydrocortisone treatment. Furthermore, we observed that the changes in serum Q-acetylglucosaminase activity in such cases were somewhat different from that of j -glucuronidase.On the other hand, several investigators (2-7) have shown an increase in serum Q glucuronidase activity of diabetic patients. Among them, however, only Woolen and Turner (6) has observed a significant correlation between plasma Q-glucuronidase activity and blood sugar levels.In the present study we have extended our survey of the relationship between serum Q-glucuronidase activity and the blood sugar level in experimental diabetic rabbits, pre pared by a single injection of alloxan or consecutive administrarion of hydrocortisone. MATERIALS AND METHODS Experimental animalsWhite male rabbits bred in our laboratories, weighing from 2.8 to 4.0 kg, were used. The animals were kept in a constant temperature (24:t2°C) and humidity controlled (50 to 70%) room.Alloxan diabetes was induced by a single intravenous injection of 100 mg/kg of re crystallized alloxan monohydrate as a 5 % solution, and cortisone diabetes was produced by the consecutive intramuscular injections of hydrocortisone acetate at a dose of 20 mg/ animal/day. Each animal was allowed to drink water ad libitum and offered 100-150 g of RC-5 (Oriental Yeast Co., Ltd., Tokyo) and 50-100 g of carrots a day, the quantities being determined by what was eaten in a few hours and sufficinet to maintain the diabetic state. The animals were placed in individual, screen-bottomed metabolism cages set over large funnels permitting quantitative collection of urine under toluen.Changes in serum (3-glucuronidase and Q-acetylglucosaminase activities in diabetic rabbits Blood sugar level, serum (3-glucuronidase and (3-acetylglucosaminase activities were determined once a week. Blood samples were drawn from the ear vein of animals in

show abstract

Section: Discussionmentioning

confidence: 99%

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Shioya

Ohishi

Kakimoto

1968

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

show abstract

“…A alfa-glicosidase está presente no intestino e é capaz de converter carboidratos complexos em carboidratos absorvíveis durante a digestão 16 . A beta-glicuronidase é uma enzima lisossômica responsável por catalisar a ligação glicosídica dos glicanos, e sua atividade está aumentada no DM, o que pode significar metabolização de glicose por uma via paralela à da insulina 17 . Já a glicerol-desidrogenase (álcool desidrogenase) atua na glicólise e no metabolismo de ácidos graxos e está implicada no aumento da glicemia via gliconeogênese 18 .…”

Section: Resultsunclassified

Triagem in silico de bioatividade de compostos encontrados na espécie Ximenia Americana

Vaz,

Bocchi,

Borges

2023

RBMC

View full text Add to dashboard Cite

Trata-se de um estudo experimental computacional (in silico) com o objetivo de elucidar os efeitos biológicos ativos de compostos encontrados na espécie X. americana (“ameixa selvagem”) e eleger as moléculas potenciais para ação biológica hipoglicemiante. Os componentes químicos da Ximenia americana foram levantados em literatura por meio dos sites Pubmed, Scielo e SienceDirect. A determinação de propriedades farmacocinéticas e de mecanismos de ação dos compostos identificados na X. americana foi feita pelas plataformas “SwissADME”, “SwissTarget”, “PASS Prediction” e “Protox II”. Para maior compreensão das atividades biológicas potenciais para efeito antidiabético, utilizou-se a base de dados “BindingDB”, associada ao servidor Pharmagist, a fim de reconhecer potenciais grupos farmacofóricos presentes na molécula candidata. Foram identificados 29 compostos químicos na espécie Ximenia americana, a partir da revisão bibliográfica. Ao se considerar as predições farmacocinética e toxicológica, apenas 3 moléculas (beta-glucogalina, quercitina-3-O-alfa-arabinofuranosídeo e alfa-D-manofuranosídeo) apresentaram-se promissoras à atividade biológica; estas com propriedades adequadas para serem candidatas à atividade biológica hipoglicemiante em seres humanos. O aumento da prevalência de síndrome metabólica percebido na sociedade atual justifica a necessidade de busca por novas moléculas, possivelmente modificadoras da doença e com menos efeitos colaterais que os fármacos atualmente utilizados. Os resultados do presente estudo favorecem desenvolvimento de futuros testes in vitro e in vivo que corroborem com as análises in silico obtidas nesta pesquisa.

show abstract

“…In a previous study of 100 participants with diabetes, β-glucuronidase activity, which indicates the regulation of a noninsulin-sensitive pathway for glucose metabolism such as the glucuronic acid cycle, was significantly higher in participants with diabetes than in the controls group [46]. Wu et al suggested that when healthy volunteers consumed konjac fiber supplement (4·to 5 g/day) for 4 weeks, that dietary supplement significantly reduced fecal β-glucuronidase activity and fecal secondary bile acid level [47].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of white sweet potato tube-feeding formula in elderly diabetic patients: a randomized controlled trial

Chen

Shih

et al. 2019

Nutr Metab (Lond)

View full text Add to dashboard Cite

Background Elderly people with type 2 diabetes mellitus (T2DM) have an increased risk of diabetes-related microvascular and macrovascular complications, thus diabetic patients with a functioning gastrointestinal tract but without sufficient oral intake require enteral nutrition (EN) formulas to control blood glucose. White sweet potato (WSP) was a kind of sweet potato could provide a healthy carbohydrate source to EN formula. The aim of this study was to examine at risk of malnutrition T2DM patients whether a WSP-EN would attenuate glucose response and elevate nutritional index compared to a standard polymeric formulas. Methods In this randomized, parallel, placebo-controlled, pilot clinical trial to investigate the effects of EN with WSP on aged residents with T2DM in long-term care institutions. In total, 54 eligible participants were randomly assigned to either the non-WSP-EN or WSP-EN group. For 60 days, the WSP-EN group received a WSP formula through nasogastric tube via a stoma with a large-bore syringe. The participants received EN of standard polymeric formulas without WSP in the non-WSP-EN group. Results The body weight, body mass index, Mini Nutritional Assessment score, and Geriatric Nutritional Risk Index were significantly higher in the WSP-EN group (p < 0.05). Moreover, the WSP-EN intervention reduced glycated hemoglobin levels (6.73% ± 1.47% vs. 6.40% ± 1.16%), but increased transferrin (223.06 ± 38.85 vs. 245.85 ± 46.08 mg/dL), high-density lipoprotein cholesterol (42.13 ± 10.56 vs. 44.25 ± 8.43 mg/dL), and vitamin A (2.45 ± 0.77 vs 2.74 ± 0.93 μM) levels (p < 0.05). In addition, there was no important side effects including gastrointestinal intolerance with prescribed doses in our WSP-EN treated patients when compared with control ones. Conclusions The results suggest WSP incorporated into enteral formulas can improve nutrition status and glycemic control in elderly diabetic patients. Trial registration NCT02711839, registered 27 May 2015.

show abstract

Increase of Serum β-Glucuronidase Activity in Human Diabetes Mellitus

Cited by 41 publications

References 8 publications

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

SERUM β-GLUCURONIDASE and β-ACETYLGLUCOSAMINASE ACTIVITIES IN EXPERIMENTAL DIABETIC RABBITS

Triagem in silico de bioatividade de compostos encontrados na espécie Ximenia Americana

Evaluation of white sweet potato tube-feeding formula in elderly diabetic patients: a randomized controlled trial

Contact Info

Product

Resources

About