Increase of mitotic activity in the colonic mucosa of patients with colorectal cancer

Abstract

A histologic and histochemical study of the colonic mucosa, including a study of the mitotic index, was performed in routinely processed specimens from control and tumor-bearing patients. A significant increase in the mitotic index (number of mitosis X 1000 gland cells), without concomitant modifications in the distribution of mitotic figures along the crypt depth, in mucosal thickness, or in mucin secretion, was demonstrated in the colonic mucosa of patients with colonic or rectal cancer compared with controls. The results point to an accelerated cell renewal in the colonic mucosa of tumor-bearing patients compared with the controls, without concomitant dysplasia. Results are discussed in the light of the possibility that an increased cell proliferation may have preceded the onset of tumor and played a role in the second step of carcinogenesis, i.e., tumor promotion, independently of dysplasia.

Long non-coding RNA TCF7 predicts the progression and facilitates the growth and metastasis of colorectal cancer

“…In the current study, lncTCF7 silencing inhibited SW-620 and HT29 cell cycle transition from G1 to S phase, which indicated that lncTCF7 silencing may inhibit SW-620 and HT29 proliferation by suppressing cell cycle progression. Proliferation and cell cycle dysfunction serve an important role in CRC development, and cell migration and invasion serve a central role in CRC metastasis (22)(23)(24). The results of the present study also indicated that lncTCF7 silencing inhibited CRC development and metastasis, indicating that lncTCF7 may function as an oncogene to promote CRC development and metastasis.…”

Increase of the mitotic index of colonic mucosa after cholecystectomy

“…The MI does not correspond exactly to the labeling index with 3H-thymidine, the latter reaching higher values than the former in the human ileum and colon. 18,20,21 In fact, cells may be labeled by 3H-thymidine, which do not divide further but are shed in the G2-phase of the cell cycle.20 Moreover, the MI depends on the relative number of mucosal cells undergoing mitosis at a given time, whereas the labeling index collects information over an interval of time. Caution must therefore be used when comparing data obtained by the two methods.…”

Real‐time polymerase chain reaction quantification of specific butyrate‐producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer

“…More than 80% of colorectal cancer occurs sporadically, having its origins in a multi‐step process of carcinogenesis involving an accumulation of multiple somatic mutations in the colonic epithelium 2 . Expansion of the proliferative zone within the colonic epithelium, secondary to luminal factors, increases the possibility of occurrence of the sequential mutations that lead to cancer 3 . The intestinal lumen is host to a very large number of bacteria and these bacteria or their metabolites are prime candidates for the etiological agents of cancer.…”