Increase of IgA-bearing Lymphocytes in Peripheral Blood from Patients with IgA Nephropathy

Nomoto, Yasuo; Sakai, Hideto; Arimori, Shigeru

doi:10.1093/ajcp/71.2.158

Cited by 88 publications

(31 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this regard, the existence of high serum levels of polymeric IgA [5], probably due to their elevated production by peripheral stimulation [6], has been demonstrated. Furthermore, a selective increase in IgA-bearing peripheral blood lym phocytes [7], a decrease of IgA-specific suppressor T cell activity [8. 9], and an IgA-specific increased activity of T helper cells have been recently shown [10], The role of all these immune cell abnormalities in the appearance and development of the IgA nephropathy is unknown.…”

Section: Introductionmentioning

confidence: 99%

Immunological Abnormalities in Healthy Relatives of Patients with IgA Nephropathy

Egido¹,

Blasco²,

Sancho³

et al. 1985

Am J Nephrol

View full text Add to dashboard Cite

Patients with IgA nephropathy have elevated serum levels of polymeric IgA, probably due to an increase in their synthesis by peripheral blood mononuclear cells, and specific abnormalities in the immunoregulation of IgA. The existence of familial cases of IgA nephropathy, as well as the published association of this nephropathy with some antigens of the HLA system, decided us to test the hypothesis that some of these alterations might be genetically controlled. For this reason we studied some of these immunological assays in 25 first-degree relatives of 7 patients with IgA nephropathy and 22 control subjects matched for age and sex. An abnormal immunological assay was found in at least 1 relative of all families examined. Thus, 16 of 25 relatives had a significant increase in the percentage of polymeric IgA-producing cells after 7 days of culture in the presence of pokeweed mitogen. Some derangement in the concanavalin A generation of specific IgA suppressor cells was found in 11 out of 25 relatives. These results are, though in lower frequency, similar to those seen in patients and suggest that the IgA-immunological abnormalities observed are genetic markers, even if they cannot by themselves explain the pathogenesis of the IgA nephropathy. The absence of IgA immune complexes seen in relatives as well as their high prevalence in patients suggest that in predisposed subjects other factors (genetic or not) are required for the development of IgA nephropathy.

show abstract

Section: Introductionmentioning

confidence: 99%

Immunological Abnormalities in Healthy Relatives of Patients with IgA Nephropathy

Egido¹,

Blasco²,

Sancho³

et al. 1985

Am J Nephrol

View full text Add to dashboard Cite

show abstract

“…There is some debate regarding the cause of the deposition. Several investigators have proposed that the elevation of the serum IgA1 concentration is the cause of IgAN (24,26). To explain why IgA1, but neither IgA2 nor IgG is deposited, it was proposed that O-glycan structures in the hinge region of IgA1 are profoundly involved in the deposition.…”

mentioning

confidence: 99%

Initiation of O-Glycan Synthesis in IgA1 Hinge Region Is Determined by a Single Enzyme, UDP-N-Acetyl-α-d-galactosamine:PolypeptideN-Acetylgalactosaminyltransferase 2

Iwasaki

Zhang

Tachibana

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

The hinge region of human immunoglobulin A1 (*IgA1) possesses multiple O-glycans, of which synthesis is initiated by the addition of GalNAc to serine or threonine through the activity of UDP-N-acetyl-␣-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases (pp-GalNAc-Ts). We found that six pp-GalNAc-Ts, ppGalNAc-T1, -T2, -T3, -T4, -T6, and -T9, were expressed in B cells, IgA-bearing B cells, and NCI-H929 IgA myeloma cells. pp-GalNAc-T activities of these six enzymes for a synthetic IgA hinge peptide, which has nine possible O-glycosylation sites, were examined using a reversed phase-high performance liquid chromatography, a matrix-assisted laser desorption ionization time of flight mass spectrometry, and peptide sequencing analysis. pp-GalNAc-T2 showed the strongest activity transferring GalNAc to a maximum of eight positions. Other pp-GalNAc-Ts exhibited different substrate specificities from pp-GalNAc-T2; however, their activities were extremely weak. It was reported that the IgA1 hinge region possesses a maximum of five O-glycans, and their amino acid positions have been determined. We found that pp-GalNAc-T2 selectively transferred GalNAc residues to the same five positions. These results strongly suggested that pp-GalNAc-T2 is an essential enzyme for initiation of O-linked glycosylation of the IgA1 hinge region.

show abstract

“…We reported earlier [30] increased levels of such IgA-bearing lympho cytes in peripheral blood in patients with IgA nephropa thy, while Fiorini et al [31] and Cosio et al [32] reported normal levels. Deviations among these reports could be eliminated by using fluorescent-activated cell sorters which eliminate any variations among individual observ ers using automated cell counting [27].…”

Section: Dr H Sakaimentioning

confidence: 66%

IgA Nephropathy in Japan

Glassock¹,

Kurokawa²,

Yoshida³

et al. 1985

Am J Nephrol

Self Cite

View full text Add to dashboard Cite

Increase of IgA-bearing Lymphocytes in Peripheral Blood from Patients with IgA Nephropathy

Cited by 88 publications

References 4 publications

Immunological Abnormalities in Healthy Relatives of Patients with IgA Nephropathy

Immunological Abnormalities in Healthy Relatives of Patients with IgA Nephropathy

Initiation of O-Glycan Synthesis in IgA1 Hinge Region Is Determined by a Single Enzyme, UDP-N-Acetyl-α-d-galactosamine:PolypeptideN-Acetylgalactosaminyltransferase 2

IgA Nephropathy in Japan

Contact Info

Product

Resources

About