Increase of apoplastic ascorbate induced by ozone is insufficient to remove the negative effects in tobacco, soybean and poplar

Abstract

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Quantifying determinants of ozone detoxification by apoplastic ascorbate in peach (Prunus persica) leaves using a model of ozone transport and reaction

“…Leaf samples were taken from two plants in each of all the OTCs on 10 August 2017 (Table 1). The leaves on the fifth or sixth branch counted from the top of plants were sampled at approximately 10:00 a.m., when the maximum value of AA apo occurred (Dai et al, 2019). The samples were analyzed for ascorbic acid and other leaf traits.…”

“…We used an infiltration‐centrifugation method to extract apoplastic fluid as described previously (Dai et al, 2019; Feng et al, 2010; Wang, Pang, Feng, Zhu, & Kobayashi, 2015). The segments of leaves were washed with distilled water, blotted dry and weighed.…”

“…Booker, Burkey, and Jones (2012) and Cheng, Burkey, Robinson, and Booker (2007) demonstrated that ASC apo pool is mostly oxidized in Arabidopsis and soybean, respectively, and therefore could not serve as an effective antioxidant. We also found that ASC apo was insufficient to protect tobacco, soybean and poplar plants from elevated concentrations of O 3 (Dai et al, 2019).…”

“…Tropospheric ozone (O 3 ) is a major air pollutant detrimental to vegetation and ecosystem productivity (Feng et al, 2019; Lefohn et al, 2018; Mills et al, 2018). Ozone around the plant canopy diffuses in the leaves through stomata, dissolves in cell wall (apoplastic) liquid, and reaches cell membrane and cytosol, where it oxidizes various cellular components exhibiting toxicity to plant tissues (Dai et al, 2019; Nouchi, Aoki, & Kobayashi, 2019a). Whereas the diffusion through stomata is a critical process that regulates the O 3 flux to plant leaves (Kangasjärvi, Jaspers, & Kollist, 2005), the reactions of O 3 with extracellular antioxidants in apoplastic liquid is another critical process controlling the amount of O 3 impinging on cell membrane (Castagna & Ranieri, 2009).…”

Importance of Detoxification Processes in Ozone Risk Assessment: Need to Integrate the Cellular Compartmentation of Antioxidants?

“…In this context, it has been integrated in different models (Polle et al, 1995;Ranieri et al, 1996;Plöchl et al, 2000;Burkey and Eason, 2002;Conklin and Barth, 2004;Tuzet et al, 2011). In fact, in many species, the importance of apoplastic AsA regarding O 3 tolerance is still unclear (D'Haese et al, 2005;Booker et al, 2012;Dai et al, 2018). For example investigated poplar clones showed no relation between apoplastic AsA content and O 3 sensitivity of clones (Van Hove et al, 2001;Di Baccio et al, 2008).…”