Increase nitric oxide and oxidative stress in dogs experimentally infected by Ehrlichia canis: Effect on the pathogenesis of the disease

Silva, Aleksandro S. Da; Munhoz, Thiago Demarchi; Faria, João Bosco; Vargas-Hérnandez, Giovanni; Machado, Rosângela Zacarias; Almeida, Taís Corrêa; Moresco, Rafael Noal; Stefani, Lenita M.; Tinucci‐Costa, Mirela

doi:10.1016/j.vetmic.2013.03.003

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…In a study on dogs infected with Ehrlichia canis, showed that, in parasitic diseases, protein damage increased along with increase in advanced oxidation protein products (AOPP) (68) NT formation, another molecular mechanism causing oxidative protein damage, is another indicator on which studies have been conducted in recent years. Peroxynitrite formation with the combination of superoxide radical and nitric oxide has various effects on both the onset and the progression of oxidative protein damage.…”

Section: Resultsmentioning

confidence: 99%

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

Kiral¹,

Sekkin²,

Paşa³

et al. 2021

Medycyna Weterynaryjna

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Leishmaniasis, considered by the World Health Organization as one of the most important zoonotic diseases, causes death when it is not treated in its self-healing skin form. The aim of this study was to investigate DNA damage and oxidative protein damage that occurs in dogs infected with Leishmania infantum. The study group consisted of 25 dogs, including 10 clinically healthy dogs aged 2 to 6 years and 15 dogs infected with Leishmania infantum diagnosed by means of the rk39 dipstick test and the immunofluorescence antibody test (IFAT). The effects of oxidative stress on protein were evaluated in the dogs infected with Leishmania infantum by determining plasma malondialdehyde, protein carbonyl groups, nitrotyrosine, and total antioxidant capacity in blood. DNA damage, on the other hand, was determined by the COMET method. Plasma protein carbonyl content (PCO) and nitrotyrosine (NT) levels, which are considered as indicators of protein damage, were found to be higher in the dogs infected with Leishmania infantum compared to the control group, but the difference in both values was not statistically significant (p > 0.05). Plasma malondialdehyde (MDA), an indicator of lipid peroxidation, was significantly higher in the dogs infected with Leishmania infantum than in the control group. TAC levels, however, were lower in the leishmanial dogs (p < 0.05). According to the results of the COMET assay, lymphocyte cells were damaged in the leishmanial dogs, and both tail intensity (TI) and tail moment (TM) values were higher in those dogs than they were in the control group (p < 0.05, p < 0.01). The parasites caused oxidative stress through protein and DNA damage in the host and decreased the antioxidant capacity concentration that prevents destructive effects.

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Section: Resultsmentioning

confidence: 99%

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

Kiral¹,

Sekkin²,

Paşa³

et al. 2021

Medycyna Weterynaryjna

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“…The presence of concurrent infection with other vector-borne pathogens or co-morbidities in dogs is considered by clinicians as infrequent or uncharacteristic disease signs. It has been suggested that oxidative stress may play a central function in the onset of clinical signs in the course of CME [ 7 , 8 ], as well as in another vector-borne disease, canine leishmaniasis [ 9 ]. Previous studies performed in experimental conditions have demonstrated a status of oxidative stress in dogs infected by Ehrlichia canis [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

et al. 2022

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The study aimed to evaluate the concentration of reactive oxidative metabolites (R-OOHs), the antioxidant barrier (OXY), and the ratio between R-OOHs and OXY (OSi) and thiol groups of plasma compounds (SHp) in in canine monocytic ehrlichiosis. Thirty dogs affected with monocytic ehrlichiosis (canine monocytic ehrlichiosis group—CME group) and ten healthy dogs (control group—CTR group) were evaluated. CME was diagnosed by the presence of clinical signs and the detection of anti-Ehlichia canis antibodies. Oxidative stress parameters of two groups were compared using the Mann–Whitney test. Significance was set at p < 0.05. Spearman rank correlation was performed to analyze oxidative stress, and hematological and biochemical variables in the CME group. All dogs affected with CME showed a wide spectrum of clinical signs such as lethargy, anorexia, fever, weight loss, lymph adenomegaly, splenomegaly, subcutaneous and mucosal petechial and ecchymosis, and vomiting. Anaemia, leukocytosis, thrombocytopenia, hyperglobulinemia, hypoalbuminemia and an increase of blood urea nitrogen and creatinine are also detected. Results showed significantly lower values of SHp in the CME group than in CTR. A statistically significant difference in the number of white blood cells, platelets, and blood urea nitrogen concentration was assayed comparing to the two groups. A negative correlation between SHp and hemoglobin concentration was recorded. These preliminary results may suggest a possible function of oxidative stress in the onset of clinical signs during the course of CME.

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“…nitric oxide) react with various organic molecules and cause lipid peroxidation, leading to extensive cell and tissue damage (Powers et al 2011). An increase of thiobarbituric acid reactive substances (TBARS) plasma levels may be considered as a sign of cellular lipid oxidation and be used as a marker of oxidative status (Da Silva et al 2013). The potential damage caused by an excess of reactive oxygen and nitrogen species is controlled by an intricate antioxidant defence system, that includes many enzymes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Natural zeolite (chabazite/phillipsite) dietary supplementation influences faecal microbiota and oxidant status of working dogs

Superchi

Saleri

Ossiprandi

et al. 2016

Italian Journal of Animal Science

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We evaluated whether chabazite/phillipsite dietary supplementation might affect the faecal microbiota, oxidant and antioxidant status of working dogs at rest undergone to a trial test. Forty English Setter dogs were involved in two replicate trials. At each replicate, dogs were divided into two homogeneous groups (10 dogs/group). During a period of 28 days, diet was supplemented (Z group) or not supplemented (C group) with chabazite/phillipsite at the dose of 5 g/head/day. On day 29, dogs were subjected to a trial test. Faecal characteristics were assessed at 0 and 29 days (within two hours from the end of the trial test). Faecal consistency was not affected by dietary supplementation (p > .05). On day 29, Lactobacillus spp. and Enterococcus spp. counts were higher and Enterobacteriaceae were lower in Z than in C group (p < .05). At day 0 and at day 29, immediately before and within one hour from the end of the trial test, blood samples were recorded to evaluate the oxidant and antioxidant status. A reduction of 40% in thiobarbituric acid reactive substances (TBARS) levels was observed in Z compared to the C group (p < .05). Differences in nitric oxide and antioxidant enzymatic system levels were not significant (p > .05). Our results suggest that chabazite/phillipsite dietary supplementation, improves the intestinal microbiota ecosystem and may counteract the oxidative damage caused by physical stress in hunting dogs at the beginning of the working season.ARTICLE HISTORY

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Increase nitric oxide and oxidative stress in dogs experimentally infected by Ehrlichia canis: Effect on the pathogenesis of the disease

Cited by 20 publications

References 30 publications

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

Investigation of DNA damage and protein damage caused by oxidative stress in canine visceral leishmaniasis

Oxidative Stress Evaluation in Dogs Affected with Canine Monocytic Ehrlichiosis

Natural zeolite (chabazite/phillipsite) dietary supplementation influences faecal microbiota and oxidant status of working dogs

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