Increase in specific opsonic activity in bovine milk following experimental Escherichia coli mastitis

Hill, Arthur W.; Heneghan, D.J.S.; Field, Terence R.; Williams, M.R.

doi:10.1016/s0034-5288(18)32183-0

Cited by 24 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the increased levels of IgG 2, no increase in opsonic activity could be detected in serum or milk samples following vaccination or the subsequent challenge, and may explain the inadequacies of mammary neutrophils to control bacterial numbers in vivo. Although IgG 2 was the predominant opsonic isotype for ovine and bovine neutrophils [5,14], IgM was also shown to have opsonic capability against S. aureus [15] and E. coli [16]. An increase in S. uberis specific IgM was also observed following experimental challenge and yet milk and serum samples collected at that time did not show enhanced opsonic activity.…”

Section: Discussionmentioning

confidence: 96%

Immune modification of the pathogenesis of Streptococcus uberis mastitis in the dairy cow

Hill¹

1994

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

Two groups of 4 cows were vaccinated subcutaneously with live Streptococcus uberis strain 0140J or a surface extract derived from the same strain, at 14 days prior to the cessation of lactation (drying off) and at calving. Both groups also received an intramammary administration of the surface extract 7 days after drying off. A third group of unvaccinated animals acted as controls. Following intramammary challenge of two quarters per cow with the vaccine strain, all quarters on control cows and those vaccinated only with surface extract developed clinical mastitis. However, only 12.5% of challenged quarters on cows which were vaccinated with live bacteria developed clinical mastitis. In addition, the numbers of bacteria in the milk following challenge were 10(5) times higher from the control and extract vaccinated cows than those which received live vaccine. Serum levels of S. uberis specific IgG2 were elevated in the animals vaccinated with the live organism when compared to that of either extract-vaccinates or controls, whilst S. uberis specific levels of IgG1 and IgM were similar in all groups throughout the experiment. Specific antibody levels in milk were unaffected by vaccination. Despite increased levels of IgG2, no increase in opsonic activity was detected in any serum or milk samples. Peripheral blood lymphocytes from animals vaccinated with live organisms showed a considerable increase in proliferative response to S. uberis antigen in vitro when compared with lymphocytes from control and extract-vaccinated animals. These results suggest that neutrophils and specific opsonising antibody may not form the major defence against infection with S. uberis.

show abstract

Section: Discussionmentioning

confidence: 96%

Immune modification of the pathogenesis of Streptococcus uberis mastitis in the dairy cow

Hill¹

1994

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Increased opsonic activity is considered to favor phagocytosis and killing by neutrophils 46 , and immunization increased the opsonic titers to some extent (Fig. 11c).…”

Section: Discussionmentioning

confidence: 99%

Local immunization impacts the response of dairy cows to Escherichia coli mastitis

Herry

Gitton

Tabouret

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Current vaccines to Escherichia coli mastitis have shown some albeit limited efficacy. Their mode of action has not been documented, and immune responses protecting the mammary gland against E. coli are not completely understood. To improve our knowledge of mammary gland immune protection, cows immunized either intramuscularly or intramammarily with the E. coli P4 were submitted to a homologous mastitis challenge. A third group of mock-immunized cows serve as challenge controls. Local immunization modified favorably the course of infection, by improving bacterial clearance while limiting inflammation. Systemic clinical signs and reduction in milk secretion were also contained. This occurred with a modification of the cytokine profile, such as an increase in IFN-γ and a reduction in TNF-α concentrations in milk. Concentrations of IL-17A and IL-22 increased in milk at the onset of the inflammatory response and remained high up to the elimination of bacteria, but concentrations did not differ between groups. Accelerated bacteriological cure was not linked to an increase in the initial efficiency of phagocytosis in milk. Results support the idea that antibodies did not play a major role in the improvement, and that cell-mediated immunity is the key to understanding E. coli vaccine-induced protection of the mammary gland.

show abstract

“…Increased speed of neutrophil recruitment into the mammary gland following vaccination of ruminants with E. coli (14) and staphylococci (1) has been reported previously. In addition, milk has been found to contain antibodies which are opsonic for E. coli (6,7) and Staphylococcus aureus (16); therefore, the enhanced inflammatory response was considered to be a benefit of such a vaccination regime. In contrast, the data presented in this report suggest that inflammation is a disadvantage when vaccinating cattle against S. uberis mastitis.…”

Section: Discussionmentioning

confidence: 99%

Local vaccination with killed Streptococcus uberis protects the bovine mammary gland against experimental intramammary challenge with the homologous strain

et al. 1994

View full text Add to dashboard Cite

The ability of killed Streptococcus uberis to induce protection against mastitis when administered either into the cistern of the dry mammary gland (intramammary vaccination) without adjuvant or subcutaneously with adjuvant was investigated. Bacteria were never reisolated from vaccinated quarters following challenge with the same strain during the subsequent lactation, and no inflammatory response was detected. In contrast, following subcutaneous vaccination, milk from challenged quarters contained very small numbers of bacteria, but these quarters did exhibit clinical disease, whereas quarters on nonvaccinated control animals produced discolored, clotted secretion with large numbers of bacteria and somatic cells and required antibiotic therapy by 60 h postchallenge. There was a significant increase in the levels of S. ubens-specific immunoglobulin Gl (IgG1), IgG2, and IgM in milk following intramammary vaccination and in the levels of specific IgGI and IgG2 in milk following subcutaneous vaccination. Levels of specific antibody in serum were also elevated following vaccination by either route. However, despite this, there was no increase in the opsonic activity of serum or milk Both peripheral blood lymphocytes and dry-period mammary gland lymphocytes showed strong proliferative responses to S. uberis in vitro following subcutaneous vaccination, but only mammary gland lymphocytes responded following intramammary vaccination. It was concluded that the protection seen in vaccinated quarters did not appear to be related to levels of specific antibody or neutrophil function and was 3599 Vol. 62, No. 9 on July 31, 2020 by guest http://iai.asm.org/ Downloaded from wall and pseudocapsule.

show abstract

Increase in specific opsonic activity in bovine milk following experimental Escherichia coli mastitis

Cited by 24 publications

References 11 publications

Immune modification of the pathogenesis of Streptococcus uberis mastitis in the dairy cow

Immune modification of the pathogenesis of Streptococcus uberis mastitis in the dairy cow

Local immunization impacts the response of dairy cows to Escherichia coli mastitis

Local vaccination with killed Streptococcus uberis protects the bovine mammary gland against experimental intramammary challenge with the homologous strain

Contact Info

Product

Resources

About