Local vaccination with killed Streptococcus uberis protects the bovine mammary gland against experimental intramammary challenge with the homologous strain

Abstract

The ability of killed Streptococcus uberis to induce protection against mastitis when administered either into the cistern of the dry mammary gland (intramammary vaccination) without adjuvant or subcutaneously with adjuvant was investigated. Bacteria were never reisolated from vaccinated quarters following challenge with the same strain during the subsequent lactation, and no inflammatory response was detected. In contrast, following subcutaneous vaccination, milk from challenged quarters contained very small numbers of bacteria, but these quarters did exhibit clinical disease, whereas quarters on nonvaccinated control animals produced discolored, clotted secretion with large numbers of bacteria and somatic cells and required antibiotic therapy by 60 h postchallenge. There was a significant increase in the levels of S. ubens-specific immunoglobulin Gl (IgG1), IgG2, and IgM in milk following intramammary vaccination and in the levels of specific IgGI and IgG2 in milk following subcutaneous vaccination. Levels of specific antibody in serum were also elevated following vaccination by either route. However, despite this, there was no increase in the opsonic activity of serum or milk Both peripheral blood lymphocytes and dry-period mammary gland lymphocytes showed strong proliferative responses to S. uberis in vitro following subcutaneous vaccination, but only mammary gland lymphocytes responded following intramammary vaccination. It was concluded that the protection seen in vaccinated quarters did not appear to be related to levels of specific antibody or neutrophil function and was 3599 Vol. 62, No. 9 on July 31, 2020 by guest http://iai.asm.org/ Downloaded from wall and pseudocapsule.

Intramammary immunization with live-attenuated Staphylococcus aureus: microbiological and immunological studies in a mouse mastitis model

“…With regard to the administration route for a staphylococcal mastitis vaccine our results agree with those of Finch et al [18], who demonstrated the efficacy of local immunization of cattle against Streptococcus uberis experimental i.ma. challenge.…”

Symposium review: Intramammary infections—Major pathogens and strain-associated complexity

“…uberis has been reported to protect against infection with the homologous strain, whereas subcutaneous vaccination resulted in partial protection. Vaccination resulted in an increase in specific IgG1, IgG2, and IgM, but there was no evidence for enhanced opsonic activity leading to increased phagocytic uptake (Finch et al, 1994). Subcutaneous vaccination of cows with live Strep.…”

Trends Intherapeutic and Prevention Strategies for Management of Bovine Mastitis: An Overview

“…The high global incidence of clinical mastitis due to S uberis, an environmental pathogen, has warranted the development of vaccines to prevent mastitis caused by this specific etiological agent [178]. Repeated immunisation in experimental animals with killed S uberis vaccine resulted in a significant reduction in the number of bacteria in milk but failed to reduce the SCC count [179]. Vaccination with bacterin from S uberis demonstrated protection against S uberis mastitis caused by the homologous strain but failed to protect against a heterologous strain [180].…”