Increase in Probing Depth Is Correlated With a Higher Number of <i>Prevotella intermedia</i> Genotypes

Alves, Ana Cláudia Braga Amoras; Napimoga, Marcelo Henrique; Klein, Marlise I.; Höfling, José Francisco; Gonçalves, Reginaldo Bruno

doi:10.1902/jop.2006.77.1.61

Cited by 6 publications

(8 citation statements)

References 31 publications

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“…Among clinical isolates, a significant number of samples were not identified as belonging to any of the species tested. This result suggested that endodontic‐periodontal lesions were multicolonized by a considerable variety of microbial agents 4,9,12,19 …”

Section: Discussionmentioning

confidence: 97%

“…In contrast, the culture consists of exhaustive, burdensome laboratory steps and also produces lower detection values compared to methods based on molecular biology in which, theoretically, much smaller numbers of microorganisms can be detected. 14,19 The lower detection values for culture methods compared to molecular biology methods was demonstrated in the comparison of results of the identification of Pi, Pg, Pn, Pe, and Tf among isolated colonies and the detection of Pg, Pe, and Tf in periodontal pockets and necrotized root canals (Tables 2 through 4). Results indicate the detection of these species through PCR analysis in anatomic sites not detected by cultures.…”

Section: Discussionmentioning

confidence: 99%

“…These analyses were performed with the aid of the program §§ . In all analyses, samples with electrophoretic profiles revealing a 95% to 100% degree of similarity were considered to be the same genotype 19 …”

Section: Methodsmentioning

confidence: 99%

“…§ § In all analyses, samples with electrophoretic profiles revealing a 95% to 100% degree of similarity were considered to be the same genotype. 19 In statistical analyses of data, the x 2 test was used to compare the prevalence of species identified in different anatomic sites (the periodontal pocket and root canal). Data were entered in a statistical software program.…”

Section: Methodsmentioning

confidence: 99%

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Detection and Clonal Analysis of Anaerobic Bacteria Associated to Endodontic‐Periodontal Lesions

Pereira

Stipp

Fonseca

et al. 2011

Journal of Periodontology

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Detection and Clonal Analysis of Anaerobic Bacteria Associated to Endodontic‐Periodontal Lesions

Pereira

Stipp

Fonseca

et al. 2011

Journal of Periodontology

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“…Increased proportions of P. intermedia and C. rectus have been associated with both the initiation and progression of the disease in other populations as well [31,32]. Alves et al [33] reported that an increase in probing depth correlated with the number of genotypes of P. intermedia . These reports suggested that P. intermedia and C. rectus are associated with advanced periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Effect of smoking on subgingival microflora of patients with periodontitis in Japan

et al. 2011

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BackgroundSmoking is a risk factor for periodontitis. To clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. The aim of this study was to gain an insight into the influence of smoking on the microflora of Japanese patients with periodontitis.MethodsSixty-seven Japanese patients with chronic periodontitis (19 to 83 years old, 23 women and 44 men) were enrolled in the present study. They consisted of 30 smokers and 37 non-smokers. Periodontal parameters including probing pocket depth (PPD) and bleeding on probing (BOP) and oral hygiene status were recorded. Detection of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum/periodonticum, Treponema denticola and Campylobacter rectus in subgingival plaque samples was performed by polymerase chain reaction. Association between the detection of periodontopathic bacteria and smoking status was analyzed by multiple logistic regression analysis and chi-square test.ResultsA statistically significant association was found between having a PPD ≥ 4 mm and detection of T. denticola, P. intermedia, T. forsythia, or C. rectus, with odds ratios ranging from 2.17 to 3.54. A significant association was noted between BOP and the detection of C. rectus or P. intermedia, and smoking, with odds ratios ranging from 1.99 to 5.62. Prevalence of C. rectus was higher in smokers than non-smokers, whereas that of A. actinomycetemcomitans was lower in smokers.ConclusionsWithin limits, the analysis of the subgingival microbial flora in smokers and non-smokers with chronic periodontitis suggests a relevant association between smoking and colonization by the specific periodontal pathogens including C. rectus.

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New antimicrobial therapies used against fungi present in subgingival sites—A brief review

Sardi

Almeida

Mendes-Giannini

2011

Archives of Oral Biology

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Although the main reservoir of Candida spp. is believed to be the buccal mucosa, these microorganisms can coaggregate with bacteria in subgingival biofilm and adhere to epithelial cells. The treatment of periodontal disease includes scaling and root planning (SRP) associated with proper oral hygiene. However, some patients may have negative responses to different therapeutic procedures, with a continuous loss of insertion, so the use of antimicrobials is needed as an adjuvant to SRP treatment. The use of a broad-spectrum antibiotic, such as tetracycline and metronidazole, as an aid in periodontal treatment has also been a factor for the development of superinfections by resistant bacteria and Candida species, even in patients with HIV. In the dental practice, the most commonly used antifungals are nystatin and fluconazole. However, the introduction of new drugs like the next generation of azoles is essential before the onset of emergent species in periodontal disease. Plants are good options for obtaining a wide variety of drugs. This alternative could benefit a large population that uses plants as a first treatment option. Plants have been used in medicine for a long time and are extensively used in folk medicine, because they represent an economic alternative, are easily accessible and are applicable to various diseases. Herein, we briefly review the literature pertaining the presence of Candida sp. in periodontal pockets, the conventional antifungal resistance and new therapies that include natural antifungal agents are reviewed.

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Increase in Probing Depth Is Correlated With a Higher Number of Prevotella intermedia Genotypes

Abstract: A clear relationship was observed between a higher number of genotypes and increase in probing depth; these results suggest that environmental challenges in the periodontal pockets may modulate the microbiota by selecting genotypes best able to exploit the environment.

Cited by 6 publications

References 31 publications

Detection and Clonal Analysis of Anaerobic Bacteria Associated to Endodontic‐Periodontal Lesions

Detection and Clonal Analysis of Anaerobic Bacteria Associated to Endodontic‐Periodontal Lesions

Effect of smoking on subgingival microflora of patients with periodontitis in Japan

New antimicrobial therapies used against fungi present in subgingival sites—A brief review

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