Increase in IS 256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants

Gregorio, Sabrina Noelia Di; Fernandez, Sebastian P.; Perazzi, Beatriz; Bello, Natalia; Famiglietti, Ángela; Mollerach, Marta

doi:10.1016/j.meegid.2016.05.001

Cited by 15 publications

(16 citation statements)

References 46 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2,4 In this study, VISA phenotype of the mutant D23C9 was stable, displayed enhanced autolysis rate, decreased pbp 4 gene expression, and a previously described loss of agr function 14 ; on the other hand, the D2P11 mutant, selected by an independent experiment, showed a nonstable VISA phenotype, reduced autolysis rate (in lysis buffer containing Triton X-100), and showed no changes in pbp 4 gene expression or agr function when compared to the parental strain D2.…”

Section: Discussionsupporting

confidence: 58%

“…Detailed clinical history of the patient was recently described. 14 In vitro selection of D2-derived mutants (D23C9, D2P11) was performed in two independent experiments by serial passage with increasing concentrations of vancomycin (Sigma-Aldrich). All strains were grown on the BHI (Britania, Argentina) broth at 37°C with aeration.…”

Section: Methodsmentioning

confidence: 99%

“…Mutant D23C9 and D2P11 were selected at 9 and 11 μg/ml of vancomycin, respectively. 14 Relevant properties of these strains are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…14 The aim of this study is to characterize the factors related to peptidoglycan metabolism in these isogenic ST100 strains.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Different Vancomycin-IntermediateStaphylococcus aureusPhenotypes Selected from the Same ST100-hVISA Parental Strain

Gregorio

Fernandez

Cuirolo

et al. 2017

Microbial Drug Resistance

Self Cite

View full text Add to dashboard Cite

The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Different Vancomycin-IntermediateStaphylococcus aureusPhenotypes Selected from the Same ST100-hVISA Parental Strain

Gregorio

Fernandez

Cuirolo

et al. 2017

Microbial Drug Resistance

Self Cite

View full text Add to dashboard Cite

show abstract

“…Transposons and transposases play important roles in the horizontal transfer of antibiotic resistance genes (Barile et al ). For example, MRSA can gain resistance to vancomycin via transposase and transposable elements (Gardete and Tomasz ; Di Gregorio et al ). A large number of transposases genes were present in the metatranscriptome, and the types and amount of transposase in R were more abundant than in C, mainly from following species such as Pseudomonas , Streptococcus pneumoniae , and Lactobacillus .…”

Section: Resultsmentioning

confidence: 99%

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

Zhang¹,

Kuang²,

Xu³

et al. 2019

Groundwater

View full text Add to dashboard Cite

Karst groundwater is an important water resource, as it accounts for about 15% of the total landscape of the earth and supplies 20% of potable water worldwide. The antibiotics resistance is an emerging global concern, and antibiotics residual and increase of antibiotic resistance genes represent serious global concerns and emerging pollutants. There is no report on the antibiotic resistance genes in groundwater. To survey resistome and microbiome in karst groundwater, two karst water samples were chosen for metagenome and metatranscriptome study, namely the 37th spring (C) and Dongcao spring (R) in Beibei, Chongqing, China. The two sites differ significantly in sulfur content, geochemical parameters, community structure, antibiotic resistance genes, and mechanisms, and these results may be influenced by anthropogenic activities. Combining with the Antibiotic Resistance Genes Database, three types of resistance genes baca, sul2, sul1 are present in R and C, and ant3ia, ermc, tetpa are also present in R. The number of all resistance genes in R was more than C, and Proteobacteria, Bacteroidetes, Nitrospirae are the main sources of antibiotic resistance genes. In addition, a large number of genes related to antibiotic gene transmission and drug resistance were found in both samples. Karst groundwater is an important source of drinking water and a possible venue for the transmission of microbial antibiotic resistance genes. However, few studies addressed this issue in karst groundwater, despite its widespread and great importance to global ecosystem. Karst groundwater is a reservoir for antibiotic resistant genes, and measures to control these resistant genes are urgently needed.

show abstract

Resistance phenotype and genetic features of a heterogeneous vancomycin intermediate–resistant Staphylococcus aureus strain from an immunocompromised patient

Cheng,

Wang,

et al. 2023

Braz J Microbiol

View full text Add to dashboard Cite

Increase in IS 256 transposition in invasive vancomycin heteroresistant Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants

Cited by 15 publications

References 46 publications

Different Vancomycin-IntermediateStaphylococcus aureusPhenotypes Selected from the Same ST100-hVISA Parental Strain

Different Vancomycin-IntermediateStaphylococcus aureusPhenotypes Selected from the Same ST100-hVISA Parental Strain

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

Resistance phenotype and genetic features of a heterogeneous vancomycin intermediate–resistant Staphylococcus aureus strain from an immunocompromised patient

Contact Info

Product

Resources

About