Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory (Cichorium intybus L.) for an antidiabetic effect

Abstract

Increase in insulin sensitivity by the association of chicoric acid and chlorogenic acid contained in a natural chicoric acid extract (NCRAE) of chicory (Cichorium intybus L.) for an antidiabetic effect, Journal of Ethnopharmacology, https://doi.org/10. 1016/j.jep.2017.12.035 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACT Ethnopharmacological relevance Chicory (Cichorium intybus L.) is an indigenous vegetable widely cultivated in Europe,America and Asia. In ancient times, the leaves, flowers, seeds, and roots have been used as a wealth of health benefits including its tonic effects, the ability to ease digestive problems and to detoxify liver. In Indian traditional therapy, chicory was known to possess antidiabetic effect. In the traditional medicine of Bulgaria and Italy, chicory was used as hypoglycemic decoctions. Aims of the studiesWe wanted to obtain the complete chemical composition of the natural chicoric acid extract (NCRAE), a chicory root extract rich in chicoric acid, which previously showed its glucose tolerance effect in normal rats. To investigate if the whole NCRAE is required to be effective, we performed a comparative in vivo experiment on STZ diabetic rats treated either with NCRAE or a mixture composed of the two major compounds of NCRAE. Materials and Methods1 K. Ferrare and L.P.R. Bidel contributed equally to this study. ResultsThe LC-MS analysis confirmed the high abundance of chicoric acid (64.2%) in NCRAE and a second part of NCRAE is composed of caffeoylquinic acids (CQAs) at 19.6% with among them the chlorogenic acid. This result has permitted us to prepare a mixture of synthetic Lchicoric acid (70%) and synthetic chlorogenic acid (30%): the solution is designated SCCAM.Our results showed that both NCRAE and SCCAM are able to improve a glucose tolerance in STZ diabetic rats after a subchronic administration of seven days. Alone NCRAE allows to significantly decrease the basal hyperglycemia after six days of treatment. In vivo experiments on streptozotocin Chicoric acid 72.4 % Caffeoyl-quinic acids 19.6 % NCRAE contained a mixture of CRA, CGA and others caffeoyl derivatives that confer this antidiabetic -NCRAE induced a antidiabetic effect, -SCCAM induced an glucose

Profiling of Altered Metabolomic States in Bidens pilosa Leaves in Response to Treatment by Methyl Jasmonate and Methyl Salicylate

“…Among the differentially perturbed B. pilosa metabolites were HCA derivatives, identified as significant ions in response to treatment with either elicitor. In previous studies, these groups of secondary metabolites were reported to have various health benefits such as anti-cancer properties [ 50 ], HIV-integrase inhibition by 3,5-di-caffeoylquinic acid [ 51 ] and chicoric acid [ 52 ] and anti-diabetic properties [ 53 , 54 ]. In this study, the di-caffeoylquinic acids, the 4,5-, 3,4- and 3,5- geometric isomers exhibited similar distribution profiles, prominent in the MeJA_12 and the MeSA_24 conditions.…”

Use of Chlorogenic Acid against Diabetes Mellitus and Its Complications

“…CA is produced in plants in the shikimic acid pathway during aerobic respiration. This compound is an ingredient, not only in foods, but also in traditional Chinese medicine preparations [4,5]. In the latter, it has been found to exert hypoglycemic [6,7], hypolipidemic [8], antibacterial [9], antioxidant [10], and anti-inflammatory [11] effects.…”

Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) protects against alloxan-induced diabetes via alleviation of oxidative stress and inflammation: Probable role of NF-kB activation

“…So, the trend towards using traditional herbal medicinal products is continuously increasing Ethanolic extracts of leaves of Annona muricata showed antidiabetic actions which might be due to the presence of many potential antioxidative phytocompounds like chlorogenic and caffeic acids, procyanidins (epi)catechin, quercetin and kaempferol (Justino et al, 2018). In streptozotocin induced diabetic rats, chicoric acid derived from roots of Cichorium intybus causes a significant reduction in basal hyperglycemic conditions with improvisation of oral glucose tolerance due to its ability to scavenge free radicals like H2O2 (Ferrare et al, 2018). Entagenic acid, an aglycone present in seed kernels of E. phaseoloides possess a significant antidiabetic effect in type 2 diabetic mice by activation of downstresm AMP-activated protein kinase – Glucose Transporter (AMPK/GLUT4) pathway (Xiong et al, 2018).…”