Increase in hepatic catalase and glycerol phosphate dehydrogenase activities on administration of clofibrate and clofenapate to the rat

Krishnakantha, T. P.; Kurup, C. K. Ramakrishna

doi:10.1042/bj1300167

Cited by 43 publications

(13 citation statements)

References 24 publications

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“…This finding is in accordance with recently published work in which the various aspects of the distributional effects of Su-13 437 (42) and Sail-42 348 (47) on peroxisomal enzymes have been comprehensively discussed. A similar compartmental redistribution of catalase activity was also encountered in the livers of CPIBtreated rats and interpreted in terms of a preferential leaching-out of catalase from PO (25,26,35,42,47), eventually leading to particles which, in a cell-free state, contain a reduced catalase complement ( Fig. 15).…”

Section: Proliferative Response Of Peroxisomessupporting

confidence: 56%

The proliferative response of hepatic peroxidomes of neonatal rats to treatment with SU-13 437 (nafenopin).

Stäubli¹,

Schweizer²,

Suter³

et al. 1977

The Journal of Cell Biology

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The repeated administration of the hypolipidemic agent Su-13 437 (nafenopin) to neonatal rats roughly doubled the number of peroxisomes in the liver tissue and caused a sixfold volumetric expansion of the peroxisomal compartment. During the proliferative response, the size-distribution of the peroxisomes was reversibly altered, enlarged particles appearing in numbers varying according to the dose given. By means of a new method for quantitative autoradiography, it was shown that (a) the concentration of silver grains over peroxisomes was comparable to that found over the endoplasmic reticulum; (b) the peak incorporation of [aH]arginine into the peroxisomes was delayed in comparison with that into the endoplasmic reticulum; (c) the label, once incorporated into the expanding peroxisomal compartment, displayed the same shift to large particles as did the whole population. These results are compatible with the biosynthetic pathway for peroxisomal catalase proposed earlier (cf. reference 12), and with the notion that the druginduced size-shift might have resulted from progressive growth of a particular class of peroxisomes formed in the presence of the agent. Evidence is presented to show that during the recovery period the larger peroxisomes are removed preferentially.In a series of reports, we have shown that two hypolipidemic drugs of the a-aryloxyisobutyric acid type, clofibrate (CPIB) 1 and CIBA Su-13 437, are endowed with the capacity to elicit the induced state and to expand the peroxisomal compartment of male rat liver parenchymal cells (24-1Abbreviations used in this paper: CPIB, clofibrate, Atromid S; DAB, diaminobenzidine; ER, endoplasmic reticulum; PO, peroxisome(s) (microbody); RER, rough-surfaced endoplasmic reticulum, SER, smoothsurfaced endoplasmic reticulum; Su-13 437, CIBA Su-13 437 (nafenopin). 26, 69). These observations were subsequently confirmed and extended by several research groups (reviewed in references 2, 30, 56, 70). The biosynthesis and precise metabolic function(s) of peroxisomes (microbodies) in normal or in experimentally altered mammalian cells are still not fully understood (12,13,56,70), although in the past few years considerable progress has been achieved with regard to the biosynthesis of peroxisomal constituents (37,38,62). In addition, interest in the biology of peroxisomes has been rekindled by a large number of morphological observations leading to the concept of the ubiquitous distribu-

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Section: Proliferative Response Of Peroxisomessupporting

confidence: 56%

The proliferative response of hepatic peroxidomes of neonatal rats to treatment with SU-13 437 (nafenopin).

Stäubli¹,

Schweizer²,

Suter³

et al. 1977

The Journal of Cell Biology

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“…254 Jena/Patnaik Thermolability of Catalase and Ageing The level of mitochondrial catalase activ ity was found to be increased with advancing age in the heart of male Wistar rats [16]. While the catalase activities of whole homoge nate and the supernatant fractions of liver and kidneys of male mice declined with age, there was no decrease in particulate (peroxisomal) catalase activity [17], The interpretation of such findings is made complicated by the reports that during extraction, catalase leaches out from peroxisomes and mitochon dria into the cytosol [2], Moreover, it appears that the subcellular distribution of catalase activity between the particulate and extrapar ticulate fractions is both species specific and organ specific. Our results indicate that in liver and kidneys, the total catalase activity of supernatants, which includes cytosolic, mito chondrial and peroxisomal fractions, remains unchanged during maturity and increases during ageing.…”

Section: Discussionmentioning

confidence: 62%

Changes in Catalase Activity and Its Thermolability in Liver and Kidneys of Ageing Male Garden Lizard

Jena¹,

Patnaik²

1992

Gerontology

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Catalase activity in liver and kidneys of male garden lizards remained unchanged during maturation, but showed an increase during ageing. Instead of inactivating catalase, thermal treatment at 60 ± 1 ° C caused a marginal increase in enzyme activity in the liver of middle-aged and kidneys of young lizards with no significant effect in old counterparts. Increase in basal enzyme activity during ageing and the maintenance of resistance against thermal inactivation of the enzyme throughout the life-span support the contention that catalase molecules in lizard tissues are not altered as a function of age, a deviation from the predictions of Orgel’s error catastrophe hypothesis.

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“…Gear reported similar findings in rats treated with clofibrate using a particle counter on isolated liver mitochondria (14). Others, however, have noted an increase in the concentration of mitochondrial protein after treatment with clofibrate or methyl clofenapate (19). An increase in the protein content of an organelle may not necessarily contradict the lack of increase in the volume or surface area of that organelle.…”

Section: Peroxisome-associated Enzymesmentioning

confidence: 96%

Morphometric analysis of the ultrastructural changes in rat liver induced by the peroxisome proliferator SaH 42-348.

Moody¹,

Reddy²

1976

The Journal of Cell Biology

127

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The changes occurring in hepatocytes of F-344 male rats during a 3-wk treatment with a hypolipidemic agent, 1-methyl-4-piperidyl-b/s[p-chlorophenoxy]acetate (Sail 42-348), have been evaluated by morphometric and biochemical methods. The twofold increase in liver weight resulted from a significant increase in hepatocyte cytoplasm as well as a moderate increase in the number of liver cells. The peroxisome population and SER played an overwhelming part in the hypertrophy of hepatocytic cytoplasm. The relative volume and the surface density of peroxisomes increased ninefold and sevenfold, respectively. The increase in the collective peroxisome volume resulted from an increase in both the number and the average volume of peroxisomes. The SER also demonstrated a substantial increase in these values. The relative volume and surface density of mitochondria were not significantly altered in comparison to controls, while these values for RER decreased onefold. Studies on the lobular distribution of cytoplasmic organeUes before and during treatment revealed that the relative volume and surface density of peroxisomes and SER increased from periportal to centrilobular cells of the hepatic lobule, whereas mitochondrial values decreased from periportal to centrilobular cells. The RER values were fairly constant in different parts of the hepatic lobule. The increase in peroxisome and SER volume and surface area was first evident within the first 3 days of Sail 42-348 treatment and these values continued to increase, reaching a steady state within 2 wk. The time course of increase in catalase and carnitine acetyltransferase activities correlated with the morphometric data on the peroxisomes. After cessation of Sail 42-348 treatment, the peroxisome values decreased rapidly within the first 3 days and reached control levels within 1 wk. Moderate reduction in SER values occurred after withdrawal of the drug, but these values remained higher than controls even after 2 wk, suggesting that the reduction in the amount of circulating peroxisome proteins may result in empty SER channels. On the 4th day of drug withdrawal a significant increase in the relative volume and surface density of lysosomes was

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Increase in hepatic catalase and glycerol phosphate dehydrogenase activities on administration of clofibrate and clofenapate to the rat

Cited by 43 publications

References 24 publications

The proliferative response of hepatic peroxidomes of neonatal rats to treatment with SU-13 437 (nafenopin).

The proliferative response of hepatic peroxidomes of neonatal rats to treatment with SU-13 437 (nafenopin).

Changes in Catalase Activity and Its Thermolability in Liver and Kidneys of Ageing Male Garden Lizard

Morphometric analysis of the ultrastructural changes in rat liver induced by the peroxisome proliferator SaH 42-348.

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