Changes in Catalase Activity and Its Thermolability in Liver and Kidneys of Ageing Male Garden Lizard

Jena, B.S.; Patnaik, B.K.

doi:10.1159/000213337

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…Thermolability at 51 ± 1°C of hepatic SDH [99] and G6PDH in liver and kidneys of gar den lizards [100] increased during ageing (ta ble 12) in support of Orgel's [97] predictions of accumulation of altered enzyme molecules with advancing age. The results are compara ble with the findings on G6PDH in various tissues of C57BL/6J mice during ageing [88], On the other hand, the hepatic catalase maintained resistance against thermal inacti vation at 60 ± 1 °C throughout the life-span, suggesting minimal alteration in enzyme mol ecules [86]. It is probable that during senes cence only selected proteins are subjected to alteration, even though the mechanism of such selective change is still unclear.…”

Section: Characteristics O F Certain Proteinssupporting

confidence: 66%

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Ageing in Reptiles

Patnaik¹

1994

Gerontology

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Like in fishes, the reptiles appear to show three types of senescence. The African skink, Mabuya buettneri, shows rapid senescence similar to death at mating observed in Salmon and marsupial mouse. Most of the lizards and snakes undergo gradual senescence comparable to the pattern exhibited by a majority of vertebrates. On the other hand, turtles, tortoises and crocodiles continue to grow throughout life and are thus credited with slow or negligible senescence. Evidences and mechanisms of rapid or negligible senescence in reptiles are still fragmentary and unclear. Findings in a few species of lizards (Calotes versicolor) and snakes (Natrix natrix) showing gradual senescence support the concept of commonalities in ageing phenomena in vertebrates. An age-related increase in the stability of collagen and accumulation of altered enzyme molecules, a decrease in metabolism and response to stress-enhanced anti-oxidative defence mechanisms and the nature of responses to hormones, restricted diet and lower environmental temperature corroborate the concept. On the other hand neither the increase in mortality rate and accumulation of lipofuscin nor the reproductive senility have been shown conclusively in ageing reptile populations. It is likely that there are multiple mechanisms of senescence in reptiles. Further studies on selected species from among the 6,000 living species are necessary to unravel the phenomena.

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Section: Characteristics O F Certain Proteinssupporting

confidence: 66%

“…In garden lizards, cerebral G6PDH [85] and hepatic catalase activities [86] remained unchanged during maturation and showed an increase during ageing (table 9). Increased G6PDH activity has been observed in ageing brain of rats [87] and mice [88].…”

Section: Anti-oxidants and Anti-oxidant Enzymesmentioning

confidence: 98%

Ageing in Reptiles

Patnaik¹

1994

Gerontology

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“…In lizard brain the inhibition increased during the maturation phase followed by a decline in older counterparts. It is possible that catalase molecules are more resistant to Al toxicity in lizards of the old age group in correlation with our earlier finding that the catalase activities of the liver and kidney in lizards showed resistance to thermal inactivation at 60°C [46]. The other reason for an age difference could be the different subcellular distributions of Al in the brain.…”

Section: Discussionsupporting

confidence: 86%

Age-Related Effect of Aluminium on the Catalase Activities of the Brains of Two Species of Poikilothermic Vertebrates

Jena

Nayak²,

Patnaik

2002

Gerontology

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Background: Although aluminium (Al) has been implicated in various neuropathological states with aging due to its involvement in neurotoxicity, the exact role of the metal ion is still unclear. Objective: The aim of the present study is to ascertain whether the antioxidant enzymes of the brain protecting from oxidative damages which accumulate with aging are regulated by Al in an age-dependent manner. Method: The inhibitory effect of Al on the catalase activity of brain homogenates of two species of poikilothermic vertebrates was studied in vitro using a spectrophotometric method. Results: At a final concentration of 666 µM, the metal ion inhibited the enzyme activity of the brain in both species. In fish brain the degree of inhibition was not age-dependent. On the other hand, the rate of inhibition increased between young and middle-aged lizards followed by a decline in the old counterparts. Conclusion: Al inhibits catalase activity but this effect may not be a major contributing factor in the aging of the brain in the two species capable of maintaining their antioxidant capacity until old age.

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“…Swimming-induced oxidative stress was evaluated by noting the activities of CAT and SOD as well as by the levels of TBARS and CD in cardiac, skeletal, hepatic and brain tissues. Catalase is one of the members in the enzymatic defense system against reactive oxygen species which catalyzes the decomposition of hydrogen peroxide (H 2 O 2 ) to water and oxygen, and thereby protects cells from oxidative damage (Jena and Patnaik, 1992). Superoxide anion, which is another reactive oxygen species, generates in oxidative stress condition and this is destroyed by SOD, another member of enzymatic defense system of cell.…”

Section: Discussionmentioning

confidence: 99%

Protection of swimming-induced oxidative stress in some vital organs by the treatment of composite extract of <i>Withania somnifera, Ocimum sanctum</i> and <i>Zingiber officinalis</i> in male rat

Misra

Maiti²,

Ghosh³

2010

Afr. J. Trad. Compl. Alt. Med.

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Composite methanolic extract of roots of Withania somnifera, leaves of Ocimum sanctum, and rhizomes of Zingiber officinalis was administered by gavage at the dose of 40 mg 100 g −1 body weight day −1 to rat orally for 15 days prior to experimentation followed by co-administration of above extract at the same dose for 28 days of swimming to find out the remedial effect of this extract on exhaustive physical exercise-induced oxidative damage. Swimming resulted significant diminution (p<0.05) in the activities of catalase and superoxide dismutase along with elevation (p<0.05) in the levels of thiobarbituric acid reactive substances and conjugated dienes in cardiac, skeletal, hepatic tissues, cerebrum and cerebellum in respect to control. The levels of all these parameters were resettled significantly (p<0.05) towards the extract pretreated cum co-treated swimming group. The antioxidative potency of this composite extract was compared with standard non-enzymatic antioxidant (vitamin-E) in forced swimming state. The above extract has no general toxic effect as reflected here from the study of transaminase activities in liver and kidney. Results lead to conclude that the composite extract of above three plant parts has a therapeutic protective effect on forced swimming-induced oxidative stress in vital organs.

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Changes in Catalase Activity and Its Thermolability in Liver and Kidneys of Ageing Male Garden Lizard

Cited by 9 publications

References 25 publications

Ageing in Reptiles

Ageing in Reptiles

Age-Related Effect of Aluminium on the Catalase Activities of the Brains of Two Species of Poikilothermic Vertebrates

Protection of swimming-induced oxidative stress in some vital organs by the treatment of composite extract of <i>Withania somnifera, Ocimum sanctum</i> and <i>Zingiber officinalis</i> in male rat

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